Limits...
17β-estradiol modulates gene expression in the female mouse cerebral cortex.

Humphreys GI, Ziegler YS, Nardulli AM - PLoS ONE (2014)

Bottom Line: Analysis of the transcriptomes of control and E2-treated animals revealed that E2 treatment significantly altered the transcript levels of 88 genes.These genes were associated with long term synaptic potentiation, myelination, phosphoprotein phosphatase activity, mitogen activated protein kinase, and phosphatidylinositol 3-kinase signaling.These results demonstrate the far-reaching and diverse effects of E2 in the cerebral cortex and provide valuable insight to begin to understand cortical processes that may fluctuate in a dynamic hormonal environment.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.

ABSTRACT
17β-estradiol (E2) plays critical roles in a number of target tissues including the mammary gland, reproductive tract, bone, and brain. Although it is clear that E2 reduces inflammation and ischemia-induced damage in the cerebral cortex, the molecular mechanisms mediating the effects of E2 in this brain region are lacking. Thus, we examined the cortical transcriptome using a mouse model system. Female adult mice were ovariectomized and implanted with silastic tubing containing oil or E2. After 7 days, the cerebral cortices were dissected and RNA was isolated and analyzed using RNA-sequencing. Analysis of the transcriptomes of control and E2-treated animals revealed that E2 treatment significantly altered the transcript levels of 88 genes. These genes were associated with long term synaptic potentiation, myelination, phosphoprotein phosphatase activity, mitogen activated protein kinase, and phosphatidylinositol 3-kinase signaling. E2 also altered the expression of genes linked to lipid synthesis and metabolism, vasoconstriction and vasodilation, cell-cell communication, and histone modification. These results demonstrate the far-reaching and diverse effects of E2 in the cerebral cortex and provide valuable insight to begin to understand cortical processes that may fluctuate in a dynamic hormonal environment.

Show MeSH

Related in: MedlinePlus

Heatmaps comparing cerebral cortices from oil- and E2-treated mice.Hierarchical clustering was used to visualize the transcript levels of (A) 88 genes that were significantly altered (FDR p<0.05) by E2 treatment or (B) 49 genes that were altered 1.2 fold or more by E2 treatment (FDR p<0.05). Each column represents cortical tissue from one mouse (8 mice total) and rows indicate genes. Colors symbolize increased (red) or decreased (blue) transcript levels.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4221195&req=5

pone-0111975-g001: Heatmaps comparing cerebral cortices from oil- and E2-treated mice.Hierarchical clustering was used to visualize the transcript levels of (A) 88 genes that were significantly altered (FDR p<0.05) by E2 treatment or (B) 49 genes that were altered 1.2 fold or more by E2 treatment (FDR p<0.05). Each column represents cortical tissue from one mouse (8 mice total) and rows indicate genes. Colors symbolize increased (red) or decreased (blue) transcript levels.

Mentions: E2 significantly (FDR p<0.05) altered the expression of 88 genes in the cerebral cortex (Table 1, Table S1). The expression of these genes is displayed on a heat map (Fig. 1A), where red indicates a significant increase and blue indicates a significant decrease in transcript levels. Interestingly, the number of genes decreased (48) by E2 treatment was slightly greater than the number of genes increased (40) by E2 treatment.


17β-estradiol modulates gene expression in the female mouse cerebral cortex.

Humphreys GI, Ziegler YS, Nardulli AM - PLoS ONE (2014)

Heatmaps comparing cerebral cortices from oil- and E2-treated mice.Hierarchical clustering was used to visualize the transcript levels of (A) 88 genes that were significantly altered (FDR p<0.05) by E2 treatment or (B) 49 genes that were altered 1.2 fold or more by E2 treatment (FDR p<0.05). Each column represents cortical tissue from one mouse (8 mice total) and rows indicate genes. Colors symbolize increased (red) or decreased (blue) transcript levels.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221195&req=5

pone-0111975-g001: Heatmaps comparing cerebral cortices from oil- and E2-treated mice.Hierarchical clustering was used to visualize the transcript levels of (A) 88 genes that were significantly altered (FDR p<0.05) by E2 treatment or (B) 49 genes that were altered 1.2 fold or more by E2 treatment (FDR p<0.05). Each column represents cortical tissue from one mouse (8 mice total) and rows indicate genes. Colors symbolize increased (red) or decreased (blue) transcript levels.
Mentions: E2 significantly (FDR p<0.05) altered the expression of 88 genes in the cerebral cortex (Table 1, Table S1). The expression of these genes is displayed on a heat map (Fig. 1A), where red indicates a significant increase and blue indicates a significant decrease in transcript levels. Interestingly, the number of genes decreased (48) by E2 treatment was slightly greater than the number of genes increased (40) by E2 treatment.

Bottom Line: Analysis of the transcriptomes of control and E2-treated animals revealed that E2 treatment significantly altered the transcript levels of 88 genes.These genes were associated with long term synaptic potentiation, myelination, phosphoprotein phosphatase activity, mitogen activated protein kinase, and phosphatidylinositol 3-kinase signaling.These results demonstrate the far-reaching and diverse effects of E2 in the cerebral cortex and provide valuable insight to begin to understand cortical processes that may fluctuate in a dynamic hormonal environment.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.

ABSTRACT
17β-estradiol (E2) plays critical roles in a number of target tissues including the mammary gland, reproductive tract, bone, and brain. Although it is clear that E2 reduces inflammation and ischemia-induced damage in the cerebral cortex, the molecular mechanisms mediating the effects of E2 in this brain region are lacking. Thus, we examined the cortical transcriptome using a mouse model system. Female adult mice were ovariectomized and implanted with silastic tubing containing oil or E2. After 7 days, the cerebral cortices were dissected and RNA was isolated and analyzed using RNA-sequencing. Analysis of the transcriptomes of control and E2-treated animals revealed that E2 treatment significantly altered the transcript levels of 88 genes. These genes were associated with long term synaptic potentiation, myelination, phosphoprotein phosphatase activity, mitogen activated protein kinase, and phosphatidylinositol 3-kinase signaling. E2 also altered the expression of genes linked to lipid synthesis and metabolism, vasoconstriction and vasodilation, cell-cell communication, and histone modification. These results demonstrate the far-reaching and diverse effects of E2 in the cerebral cortex and provide valuable insight to begin to understand cortical processes that may fluctuate in a dynamic hormonal environment.

Show MeSH
Related in: MedlinePlus