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Estimation of glomerular filtration rate in cynomolgus monkeys (Macaca fascicularis).

Iwama R, Sato T, Sakurai K, Takasuna K, Ichijo T, Furuhama K, Satoh H - J. Vet. Med. Sci. (2014)

Bottom Line: To estimate the glomerular filtration rate (GFR) in cynomolgus monkeys (Macaca fascicularis), a three-blood-sample method using iodixanol was assessed in comparison with the conventional multisample strategy using inulin.A close correlation (r=0.96) was noted between the GFR values estimated by both methods.This is the first report, suggesting that serum clearance of iodixanol is a ready-to-use tool for a screening the GFR in monkeys, although it is necessary to perform a more longitudinal study using animals with reduced renal function.

View Article: PubMed Central - PubMed

Affiliation: Cooperative Department of Veterinary Basic Medicine, Iwate University, Morioka 020-86550, Japan.

ABSTRACT
To estimate the glomerular filtration rate (GFR) in cynomolgus monkeys (Macaca fascicularis), a three-blood-sample method using iodixanol was assessed in comparison with the conventional multisample strategy using inulin. Iodixanol and inulin were coadministered intravenously 40 mg I/kg and 50 mg/kg, respectively, to male monkeys, followed by blood collection 60, 90 and 120 min later. A close correlation (r=0.96) was noted between the GFR values estimated by both methods. In clinically healthy monkeys, the basal values were determined to be 3.06 ± 0.50 ml/min/kg. This is the first report, suggesting that serum clearance of iodixanol is a ready-to-use tool for a screening the GFR in monkeys, although it is necessary to perform a more longitudinal study using animals with reduced renal function.

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Plots between GFR values estimated by the multisample method using iodixanol (iodixanolGFR) and inulin (inulin GFR) in healthy cynomolgus monkeys. (A) Scatter plot of GFRbetween the two methods. Deming’s regression was y=1.18×–0.54. r=0.96.(B) Bland and Altman plot of the differences between the two methods. Mean bias (solidline): 0.04. Upper and lower values represent 95% agreement plots: mean bias ± 0.51(dotted lines). n=8.
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fig_002: Plots between GFR values estimated by the multisample method using iodixanol (iodixanolGFR) and inulin (inulin GFR) in healthy cynomolgus monkeys. (A) Scatter plot of GFRbetween the two methods. Deming’s regression was y=1.18×–0.54. r=0.96.(B) Bland and Altman plot of the differences between the two methods. Mean bias (solidline): 0.04. Upper and lower values represent 95% agreement plots: mean bias ± 0.51(dotted lines). n=8.

Mentions: When iodixanol and inulin were coadministered intravenously at 40 mg I/kg and 50 mg/kg,respectively, to 8 healthy monkeys, there was a close correlation (r=0.96,P<0.01) between their GFR values (Fig. 2AFig. 2.


Estimation of glomerular filtration rate in cynomolgus monkeys (Macaca fascicularis).

Iwama R, Sato T, Sakurai K, Takasuna K, Ichijo T, Furuhama K, Satoh H - J. Vet. Med. Sci. (2014)

Plots between GFR values estimated by the multisample method using iodixanol (iodixanolGFR) and inulin (inulin GFR) in healthy cynomolgus monkeys. (A) Scatter plot of GFRbetween the two methods. Deming’s regression was y=1.18×–0.54. r=0.96.(B) Bland and Altman plot of the differences between the two methods. Mean bias (solidline): 0.04. Upper and lower values represent 95% agreement plots: mean bias ± 0.51(dotted lines). n=8.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221181&req=5

fig_002: Plots between GFR values estimated by the multisample method using iodixanol (iodixanolGFR) and inulin (inulin GFR) in healthy cynomolgus monkeys. (A) Scatter plot of GFRbetween the two methods. Deming’s regression was y=1.18×–0.54. r=0.96.(B) Bland and Altman plot of the differences between the two methods. Mean bias (solidline): 0.04. Upper and lower values represent 95% agreement plots: mean bias ± 0.51(dotted lines). n=8.
Mentions: When iodixanol and inulin were coadministered intravenously at 40 mg I/kg and 50 mg/kg,respectively, to 8 healthy monkeys, there was a close correlation (r=0.96,P<0.01) between their GFR values (Fig. 2AFig. 2.

Bottom Line: To estimate the glomerular filtration rate (GFR) in cynomolgus monkeys (Macaca fascicularis), a three-blood-sample method using iodixanol was assessed in comparison with the conventional multisample strategy using inulin.A close correlation (r=0.96) was noted between the GFR values estimated by both methods.This is the first report, suggesting that serum clearance of iodixanol is a ready-to-use tool for a screening the GFR in monkeys, although it is necessary to perform a more longitudinal study using animals with reduced renal function.

View Article: PubMed Central - PubMed

Affiliation: Cooperative Department of Veterinary Basic Medicine, Iwate University, Morioka 020-86550, Japan.

ABSTRACT
To estimate the glomerular filtration rate (GFR) in cynomolgus monkeys (Macaca fascicularis), a three-blood-sample method using iodixanol was assessed in comparison with the conventional multisample strategy using inulin. Iodixanol and inulin were coadministered intravenously 40 mg I/kg and 50 mg/kg, respectively, to male monkeys, followed by blood collection 60, 90 and 120 min later. A close correlation (r=0.96) was noted between the GFR values estimated by both methods. In clinically healthy monkeys, the basal values were determined to be 3.06 ± 0.50 ml/min/kg. This is the first report, suggesting that serum clearance of iodixanol is a ready-to-use tool for a screening the GFR in monkeys, although it is necessary to perform a more longitudinal study using animals with reduced renal function.

Show MeSH
Related in: MedlinePlus