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Evaluation of selected cytokine gene expression in colonic mucosa from dogs with idiopathic lymphocytic-plasmacytic colitis.

Tamura Y, Ohta H, Yokoyama N, Lim SY, Osuga T, Morishita K, Nakamura K, Yamasaki M, Takiguchi M - J. Vet. Med. Sci. (2014)

Bottom Line: In this study, we investigated mRNA transcription levels of T helper cell cytokines, such as IFN-γ, IL-4, IL-17 and IL-10 and pro-inflammatory cytokines, such as IL-1β, IL-6, TNF-α, IL-8, IL-12 and IL-23, in colonic mucosa from LPC dogs by quantitative real-time RT-PCR.No significant differences were detected in cytokine mRNA expressions between dogs with LPC and controls, except for IL-23p19.Dogs with LPC failed to express a predominant cytokine profile in inflamed colonic mucosa as opposed to human IBD.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.

ABSTRACT
Lymphocytic-plasmacytic colitis (LPC) is a common form of inflammatory bowel disease (IBD) affecting the canine large intestine. Cytokines are thought to be involved in the pathogenesis of IBD. However, to date, few studies have investigated cytokine mRNA expression in dogs with LPC. In this study, we investigated mRNA transcription levels of T helper cell cytokines, such as IFN-γ, IL-4, IL-17 and IL-10 and pro-inflammatory cytokines, such as IL-1β, IL-6, TNF-α, IL-8, IL-12 and IL-23, in colonic mucosa from LPC dogs by quantitative real-time RT-PCR. No significant differences were detected in cytokine mRNA expressions between dogs with LPC and controls, except for IL-23p19. Dogs with LPC failed to express a predominant cytokine profile in inflamed colonic mucosa as opposed to human IBD.

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Relative expression of cytokine (IFN-γ, IL-4, IL-10, IL-17, IL-1β, IL-6, TNF-α, IL-8,IL-12p35, IL-12/23p40 and IL-23p19) mRNA in colonic specimens from healthy controls (HC,n=12) and dogs with LPC (LPC, n=6). Transcription of succinate dehydrogenase complex,subunit A (SDHA) mRNA was used as an internal control. The horizontallines correspond to the median of the relative quantity in each group.
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fig_001: Relative expression of cytokine (IFN-γ, IL-4, IL-10, IL-17, IL-1β, IL-6, TNF-α, IL-8,IL-12p35, IL-12/23p40 and IL-23p19) mRNA in colonic specimens from healthy controls (HC,n=12) and dogs with LPC (LPC, n=6). Transcription of succinate dehydrogenase complex,subunit A (SDHA) mRNA was used as an internal control. The horizontallines correspond to the median of the relative quantity in each group.

Mentions: No significant differences were detected in cytokine mRNA expressions between dogs with LPCand control dogs, except for IL-23p19 (Fig. 1Fig. 1.


Evaluation of selected cytokine gene expression in colonic mucosa from dogs with idiopathic lymphocytic-plasmacytic colitis.

Tamura Y, Ohta H, Yokoyama N, Lim SY, Osuga T, Morishita K, Nakamura K, Yamasaki M, Takiguchi M - J. Vet. Med. Sci. (2014)

Relative expression of cytokine (IFN-γ, IL-4, IL-10, IL-17, IL-1β, IL-6, TNF-α, IL-8,IL-12p35, IL-12/23p40 and IL-23p19) mRNA in colonic specimens from healthy controls (HC,n=12) and dogs with LPC (LPC, n=6). Transcription of succinate dehydrogenase complex,subunit A (SDHA) mRNA was used as an internal control. The horizontallines correspond to the median of the relative quantity in each group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221177&req=5

fig_001: Relative expression of cytokine (IFN-γ, IL-4, IL-10, IL-17, IL-1β, IL-6, TNF-α, IL-8,IL-12p35, IL-12/23p40 and IL-23p19) mRNA in colonic specimens from healthy controls (HC,n=12) and dogs with LPC (LPC, n=6). Transcription of succinate dehydrogenase complex,subunit A (SDHA) mRNA was used as an internal control. The horizontallines correspond to the median of the relative quantity in each group.
Mentions: No significant differences were detected in cytokine mRNA expressions between dogs with LPCand control dogs, except for IL-23p19 (Fig. 1Fig. 1.

Bottom Line: In this study, we investigated mRNA transcription levels of T helper cell cytokines, such as IFN-γ, IL-4, IL-17 and IL-10 and pro-inflammatory cytokines, such as IL-1β, IL-6, TNF-α, IL-8, IL-12 and IL-23, in colonic mucosa from LPC dogs by quantitative real-time RT-PCR.No significant differences were detected in cytokine mRNA expressions between dogs with LPC and controls, except for IL-23p19.Dogs with LPC failed to express a predominant cytokine profile in inflamed colonic mucosa as opposed to human IBD.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.

ABSTRACT
Lymphocytic-plasmacytic colitis (LPC) is a common form of inflammatory bowel disease (IBD) affecting the canine large intestine. Cytokines are thought to be involved in the pathogenesis of IBD. However, to date, few studies have investigated cytokine mRNA expression in dogs with LPC. In this study, we investigated mRNA transcription levels of T helper cell cytokines, such as IFN-γ, IL-4, IL-17 and IL-10 and pro-inflammatory cytokines, such as IL-1β, IL-6, TNF-α, IL-8, IL-12 and IL-23, in colonic mucosa from LPC dogs by quantitative real-time RT-PCR. No significant differences were detected in cytokine mRNA expressions between dogs with LPC and controls, except for IL-23p19. Dogs with LPC failed to express a predominant cytokine profile in inflamed colonic mucosa as opposed to human IBD.

Show MeSH
Related in: MedlinePlus