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Proteomic identification of proteins differentially expressed in response to resveratrol treatment in middle cerebral artery occlusion stroke model.

Shah FA, Gim SA, Kim MO, Koh PO - J. Vet. Med. Sci. (2014)

Bottom Line: Several proteins were identified as differentially expressed between vehicle- and resveratrol-treated animals.Among these proteins, expression of peroxiredoxin-5, isocitrate dehydrogenase [NAD(+)], apolipoprotein A-I and ubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group, whereas resveratrol attenuated the injury-induced decrease in expression of these proteins.However, expression of collapsing response mediator protein 2 was increased in the vehicle-treated group, whereas resveratrol prevented the injury-induced increase in the expression of this protein.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.

ABSTRACT
Resveratrol has a neuroprotective effect against cerebral ischemia. The objective of this study was to identify proteins that are differentially expressed in the cerebral cortex of vehicle- and resveratrol-treated animals during ischemic injury. Focal cerebral ischemia was induced as middle cerebral artery occlusion (MCAO) in male rats. Rats were treated with vehicle or resveratrol before MCAO, and cerebral cortex was collected 24 hr after MCAO. Cerebral cortex proteins were identified by two-dimensional gel electrophoresis and mass spectrometry. Several proteins were identified as differentially expressed between vehicle- and resveratrol-treated animals. Among these proteins, expression of peroxiredoxin-5, isocitrate dehydrogenase [NAD(+)], apolipoprotein A-I and ubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group, whereas resveratrol attenuated the injury-induced decrease in expression of these proteins. However, expression of collapsing response mediator protein 2 was increased in the vehicle-treated group, whereas resveratrol prevented the injury-induced increase in the expression of this protein. These findings suggest that resveratrol modulates the expression of various proteins that associated with oxidative stress and energy metabolism in focal cerebral ischemia.

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Two-dimensional SDS-PAGE analysis of proteins in the cerebral cortex fromsham-operated (A and B), vehicle-treated (C and D) and resveratrol-treated (E and F)animals after middle cerebral artery occlusion (MCAO). Isoelectric focusing wasperformed at pH 4–7 and pH 6–9 using IPG strips, followed by second-dimensionalseparation on 7.5–17.5% gradient SDS gels stained with silver. Squares indicate theprotein spots that were differentially expressed between vehicle- andresveratrol-treated groups.
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fig_001: Two-dimensional SDS-PAGE analysis of proteins in the cerebral cortex fromsham-operated (A and B), vehicle-treated (C and D) and resveratrol-treated (E and F)animals after middle cerebral artery occlusion (MCAO). Isoelectric focusing wasperformed at pH 4–7 and pH 6–9 using IPG strips, followed by second-dimensionalseparation on 7.5–17.5% gradient SDS gels stained with silver. Squares indicate theprotein spots that were differentially expressed between vehicle- andresveratrol-treated groups.

Mentions: Two-dimensional electrophoresis (2-DE) maps were generated for cerebral cortex proteinspots from sham-operated, vehicle-treated and resveratrol-treated animals (pH range 4–7 andpH 6–9). Approximately 980 protein spots per image were observed. We detected thirty-fourprotein spots with more than a 2.5-fold change in intensity. Among these protein spots,twenty-nine proteins were clearly identified by MALDI-TOF analysis with a protein sequencecoverage ranging from 21–67% (Fig. 1Fig. 1.


Proteomic identification of proteins differentially expressed in response to resveratrol treatment in middle cerebral artery occlusion stroke model.

Shah FA, Gim SA, Kim MO, Koh PO - J. Vet. Med. Sci. (2014)

Two-dimensional SDS-PAGE analysis of proteins in the cerebral cortex fromsham-operated (A and B), vehicle-treated (C and D) and resveratrol-treated (E and F)animals after middle cerebral artery occlusion (MCAO). Isoelectric focusing wasperformed at pH 4–7 and pH 6–9 using IPG strips, followed by second-dimensionalseparation on 7.5–17.5% gradient SDS gels stained with silver. Squares indicate theprotein spots that were differentially expressed between vehicle- andresveratrol-treated groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221170&req=5

fig_001: Two-dimensional SDS-PAGE analysis of proteins in the cerebral cortex fromsham-operated (A and B), vehicle-treated (C and D) and resveratrol-treated (E and F)animals after middle cerebral artery occlusion (MCAO). Isoelectric focusing wasperformed at pH 4–7 and pH 6–9 using IPG strips, followed by second-dimensionalseparation on 7.5–17.5% gradient SDS gels stained with silver. Squares indicate theprotein spots that were differentially expressed between vehicle- andresveratrol-treated groups.
Mentions: Two-dimensional electrophoresis (2-DE) maps were generated for cerebral cortex proteinspots from sham-operated, vehicle-treated and resveratrol-treated animals (pH range 4–7 andpH 6–9). Approximately 980 protein spots per image were observed. We detected thirty-fourprotein spots with more than a 2.5-fold change in intensity. Among these protein spots,twenty-nine proteins were clearly identified by MALDI-TOF analysis with a protein sequencecoverage ranging from 21–67% (Fig. 1Fig. 1.

Bottom Line: Several proteins were identified as differentially expressed between vehicle- and resveratrol-treated animals.Among these proteins, expression of peroxiredoxin-5, isocitrate dehydrogenase [NAD(+)], apolipoprotein A-I and ubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group, whereas resveratrol attenuated the injury-induced decrease in expression of these proteins.However, expression of collapsing response mediator protein 2 was increased in the vehicle-treated group, whereas resveratrol prevented the injury-induced increase in the expression of this protein.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.

ABSTRACT
Resveratrol has a neuroprotective effect against cerebral ischemia. The objective of this study was to identify proteins that are differentially expressed in the cerebral cortex of vehicle- and resveratrol-treated animals during ischemic injury. Focal cerebral ischemia was induced as middle cerebral artery occlusion (MCAO) in male rats. Rats were treated with vehicle or resveratrol before MCAO, and cerebral cortex was collected 24 hr after MCAO. Cerebral cortex proteins were identified by two-dimensional gel electrophoresis and mass spectrometry. Several proteins were identified as differentially expressed between vehicle- and resveratrol-treated animals. Among these proteins, expression of peroxiredoxin-5, isocitrate dehydrogenase [NAD(+)], apolipoprotein A-I and ubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group, whereas resveratrol attenuated the injury-induced decrease in expression of these proteins. However, expression of collapsing response mediator protein 2 was increased in the vehicle-treated group, whereas resveratrol prevented the injury-induced increase in the expression of this protein. These findings suggest that resveratrol modulates the expression of various proteins that associated with oxidative stress and energy metabolism in focal cerebral ischemia.

Show MeSH
Related in: MedlinePlus