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Vasomotor effects of acetylcholine, bradykinin, noradrenaline, 5-hydroxytryptamine, histamine and angiotensin II on the mouse basilar artery.

Islam MZ, Watanabe Y, Nguyen HT, Yamazaki-Himeno E, Obi T, Shiraishi M, Miyamoto A - J. Vet. Med. Sci. (2014)

Bottom Line: NA failed to produce any vasomotor action.Diphenhydramine (a H1 antagonist) shifted the concentration-response curve for His to the right, whereas cimetidine (a H2 antagonist) had no significant effect.Losartan (an AT1 antagonist) shifted the concentration-response curve for Ang II to the right, whereas PD123319 (an AT2 antagonist) had no significant effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

ABSTRACT
We investigated the responsiveness of the mouse basilar artery to acetylcholine (ACh), bradykinin (BK), noradrenaline (NA), 5-hydroxytryptamine (5-HT), histamine (His) and angiotensin (Ang) II in order to characterize the related receptor subtypes in vitro. ACh and BK induced endothelium-dependent relaxation of precontracted arteries with U-46619 (a thromboxane A2 analogue). Atropine (a non-selective muscarinic receptor antagonist) and NĪ‰-nitro-L-arginine (a NO synthase inhibitor, L-NNA) shifted the concentration-response curve for ACh to the right, whereas pirenzepine, methoctramine and pFHHSiD (muscarinic M1, M2 and M3 antagonists, respectively) had no significant effect. L-NNA and HOE140 (a B2 antagonist) shifted the concentration-response curve for BK to the right, whereas des-Arg(9)-[Leu(8)]-BK (a B1 antagonist) and indomethacin (a cyclooxygenase inhibitor) had no significant effect. NA failed to produce any vasomotor action. His and Ang II induced concentration-dependent contraction. Diphenhydramine (a H1 antagonist) shifted the concentration-response curve for His to the right, whereas cimetidine (a H2 antagonist) had no significant effect. Losartan (an AT1 antagonist) shifted the concentration-response curve for Ang II to the right, whereas PD123319 (an AT2 antagonist) had no significant effect. These results suggest that the H1 and AT1 receptor subtypes might play an important role in arterial contraction, whereas muscarinic receptor subtypes apart from M1, M2 and M3, and B2 receptors on the endothelium, might modify these contractions to relaxations.

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Effect of the AT1 receptor antagonist losartan (■ 10−7 M, Δ10−6 M) and the AT2 receptor antagonist PD123319 (○,10−6 M) on angiotensin (Ang) II-induced contraction (●) [A] and Schildplot of losartan [B] in the isolated mouse basilar artery. The maximum contractioninduced by Ang II in the absence of antagonist was taken as 100%. Each pointrepresents the mean ± SEM of 7 mice. CR: see Fig.2.
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fig_006: Effect of the AT1 receptor antagonist losartan (■ 10−7 M, Δ10−6 M) and the AT2 receptor antagonist PD123319 (○,10−6 M) on angiotensin (Ang) II-induced contraction (●) [A] and Schildplot of losartan [B] in the isolated mouse basilar artery. The maximum contractioninduced by Ang II in the absence of antagonist was taken as 100%. Each pointrepresents the mean ± SEM of 7 mice. CR: see Fig.2.

Mentions: Effects of losartan and PD123319 on Ang II-induced contraction: Weexamined the effects of losartan (an AT1 receptor antagonist) and PD123319 (anAT2 receptor antagonist) on the concentration-response curve for Ang II (Fig. 6Fig. 6.


Vasomotor effects of acetylcholine, bradykinin, noradrenaline, 5-hydroxytryptamine, histamine and angiotensin II on the mouse basilar artery.

Islam MZ, Watanabe Y, Nguyen HT, Yamazaki-Himeno E, Obi T, Shiraishi M, Miyamoto A - J. Vet. Med. Sci. (2014)

Effect of the AT1 receptor antagonist losartan (■ 10−7 M, Δ10−6 M) and the AT2 receptor antagonist PD123319 (○,10−6 M) on angiotensin (Ang) II-induced contraction (●) [A] and Schildplot of losartan [B] in the isolated mouse basilar artery. The maximum contractioninduced by Ang II in the absence of antagonist was taken as 100%. Each pointrepresents the mean ± SEM of 7 mice. CR: see Fig.2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221166&req=5

fig_006: Effect of the AT1 receptor antagonist losartan (■ 10−7 M, Δ10−6 M) and the AT2 receptor antagonist PD123319 (○,10−6 M) on angiotensin (Ang) II-induced contraction (●) [A] and Schildplot of losartan [B] in the isolated mouse basilar artery. The maximum contractioninduced by Ang II in the absence of antagonist was taken as 100%. Each pointrepresents the mean ± SEM of 7 mice. CR: see Fig.2.
Mentions: Effects of losartan and PD123319 on Ang II-induced contraction: Weexamined the effects of losartan (an AT1 receptor antagonist) and PD123319 (anAT2 receptor antagonist) on the concentration-response curve for Ang II (Fig. 6Fig. 6.

Bottom Line: NA failed to produce any vasomotor action.Diphenhydramine (a H1 antagonist) shifted the concentration-response curve for His to the right, whereas cimetidine (a H2 antagonist) had no significant effect.Losartan (an AT1 antagonist) shifted the concentration-response curve for Ang II to the right, whereas PD123319 (an AT2 antagonist) had no significant effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

ABSTRACT
We investigated the responsiveness of the mouse basilar artery to acetylcholine (ACh), bradykinin (BK), noradrenaline (NA), 5-hydroxytryptamine (5-HT), histamine (His) and angiotensin (Ang) II in order to characterize the related receptor subtypes in vitro. ACh and BK induced endothelium-dependent relaxation of precontracted arteries with U-46619 (a thromboxane A2 analogue). Atropine (a non-selective muscarinic receptor antagonist) and NĪ‰-nitro-L-arginine (a NO synthase inhibitor, L-NNA) shifted the concentration-response curve for ACh to the right, whereas pirenzepine, methoctramine and pFHHSiD (muscarinic M1, M2 and M3 antagonists, respectively) had no significant effect. L-NNA and HOE140 (a B2 antagonist) shifted the concentration-response curve for BK to the right, whereas des-Arg(9)-[Leu(8)]-BK (a B1 antagonist) and indomethacin (a cyclooxygenase inhibitor) had no significant effect. NA failed to produce any vasomotor action. His and Ang II induced concentration-dependent contraction. Diphenhydramine (a H1 antagonist) shifted the concentration-response curve for His to the right, whereas cimetidine (a H2 antagonist) had no significant effect. Losartan (an AT1 antagonist) shifted the concentration-response curve for Ang II to the right, whereas PD123319 (an AT2 antagonist) had no significant effect. These results suggest that the H1 and AT1 receptor subtypes might play an important role in arterial contraction, whereas muscarinic receptor subtypes apart from M1, M2 and M3, and B2 receptors on the endothelium, might modify these contractions to relaxations.

Show MeSH
Related in: MedlinePlus