Limits...
Isolation and characterization of marine Brevibacillus sp. S-1 collected from South China Sea and a novel antitumor peptide produced by the strain.

Zheng L, Yi Y, Liu J, Lin X, Yang K, Lv M, Zhou X, Hao J, Liu J, Zheng Y, Sun M - PLoS ONE (2014)

Bottom Line: S-1, using ion-exchange chromatography and reverse-phase HPLC chromatography.Additionally, SBP exhibited low cytotoxicity against HFL1 human normal fibroblast lung cells.The result suggested that the cytotoxic effect of the peptide is specific to tumor cells.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China.

ABSTRACT
A Gram-positive, rod-shaped bacterium, designated as S-1, was isolated from a marine sediment sample collected from South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence showed that S-1 belongs to the genus Brevibacillus. A novel cytotoxic peptide was isolated from the fermentation broth of the marine-derived bacterium Brevibacillus sp. S-1, using ion-exchange chromatography and reverse-phase HPLC chromatography. The molecular weight of this peptide was determined as 1570 Da by MALDI-TOF mass spectrometry, and its structure was proposed as a cyclic peptide elucidated by MALDI-TOF/TOF mass spectrometry and de novo sequencing. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this peptide exhibited cytotoxicity against BEL-7402 human hepatocellular carcinoma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, U251 human glioma cells and MCF-7 human breast carcinoma cells. Additionally, SBP exhibited low cytotoxicity against HFL1 human normal fibroblast lung cells. The result suggested that the cytotoxic effect of the peptide is specific to tumor cells.

Show MeSH

Related in: MedlinePlus

Phylogenetic dendrogram of Brevibacillus sp. S-1 and its related species based on 16S rRNA gene sequence similarities.The tree was constructed using the neighbour-joining method implemented in the program MEGA version 6. Bar, 0.01 nt substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4220994&req=5

pone-0111270-g001: Phylogenetic dendrogram of Brevibacillus sp. S-1 and its related species based on 16S rRNA gene sequence similarities.The tree was constructed using the neighbour-joining method implemented in the program MEGA version 6. Bar, 0.01 nt substitutions per site.

Mentions: Marine microorganisms are a major source for natural products. Now, 16 of 20 marine antitumor compounds under clinical trial are derived from microbial sources [22]. Therefore, isolation and cultivation of a new marine microorganism may be a shortcut to discover novel natural products [23]. A variety of pretreatment methods including enriching physical and chemical techniques are employed to favor the isolation of specific marine microorganisms, especially less abundant bacteria. [24], [25]. Anticancer ε -poly-L-lysine (ε -PL) was produced by a marine Bacillus subtilis sp. isolated from sea water in Alexandria [26]. We isolated a strain, S-1, from the bottom sediments of South China Sea. The near-complete 16S rRNA gene sequence (1466 nt) of strain S-1 was submitted to GeneBank (GenBank accession number KC871055). Phylogenetic analysis based on the 16S rRNA sequence indicated that strain S-1 belonged to the genus Brevibacillus, with the highest sequence similarities to Brevibacillus laterosporus DSM25 (98.29%), Brevibacillus panacihumi DCY35 (97.26%), Brevibacillus invocatus NCIMB 13772 (96.99%), Brevibacillus fluminis CJ71 (96.89%) and Brevibacillus centrosporus DSM 8445 (96.78%). The neighbour-joining phylogenetic tree further confirmed that strain S-1 was phylogenetically related to genus Brevibacillus and formed a clade with Brevibacillus laterosporus (Figure 1).


Isolation and characterization of marine Brevibacillus sp. S-1 collected from South China Sea and a novel antitumor peptide produced by the strain.

Zheng L, Yi Y, Liu J, Lin X, Yang K, Lv M, Zhou X, Hao J, Liu J, Zheng Y, Sun M - PLoS ONE (2014)

Phylogenetic dendrogram of Brevibacillus sp. S-1 and its related species based on 16S rRNA gene sequence similarities.The tree was constructed using the neighbour-joining method implemented in the program MEGA version 6. Bar, 0.01 nt substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4220994&req=5

pone-0111270-g001: Phylogenetic dendrogram of Brevibacillus sp. S-1 and its related species based on 16S rRNA gene sequence similarities.The tree was constructed using the neighbour-joining method implemented in the program MEGA version 6. Bar, 0.01 nt substitutions per site.
Mentions: Marine microorganisms are a major source for natural products. Now, 16 of 20 marine antitumor compounds under clinical trial are derived from microbial sources [22]. Therefore, isolation and cultivation of a new marine microorganism may be a shortcut to discover novel natural products [23]. A variety of pretreatment methods including enriching physical and chemical techniques are employed to favor the isolation of specific marine microorganisms, especially less abundant bacteria. [24], [25]. Anticancer ε -poly-L-lysine (ε -PL) was produced by a marine Bacillus subtilis sp. isolated from sea water in Alexandria [26]. We isolated a strain, S-1, from the bottom sediments of South China Sea. The near-complete 16S rRNA gene sequence (1466 nt) of strain S-1 was submitted to GeneBank (GenBank accession number KC871055). Phylogenetic analysis based on the 16S rRNA sequence indicated that strain S-1 belonged to the genus Brevibacillus, with the highest sequence similarities to Brevibacillus laterosporus DSM25 (98.29%), Brevibacillus panacihumi DCY35 (97.26%), Brevibacillus invocatus NCIMB 13772 (96.99%), Brevibacillus fluminis CJ71 (96.89%) and Brevibacillus centrosporus DSM 8445 (96.78%). The neighbour-joining phylogenetic tree further confirmed that strain S-1 was phylogenetically related to genus Brevibacillus and formed a clade with Brevibacillus laterosporus (Figure 1).

Bottom Line: S-1, using ion-exchange chromatography and reverse-phase HPLC chromatography.Additionally, SBP exhibited low cytotoxicity against HFL1 human normal fibroblast lung cells.The result suggested that the cytotoxic effect of the peptide is specific to tumor cells.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China.

ABSTRACT
A Gram-positive, rod-shaped bacterium, designated as S-1, was isolated from a marine sediment sample collected from South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence showed that S-1 belongs to the genus Brevibacillus. A novel cytotoxic peptide was isolated from the fermentation broth of the marine-derived bacterium Brevibacillus sp. S-1, using ion-exchange chromatography and reverse-phase HPLC chromatography. The molecular weight of this peptide was determined as 1570 Da by MALDI-TOF mass spectrometry, and its structure was proposed as a cyclic peptide elucidated by MALDI-TOF/TOF mass spectrometry and de novo sequencing. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this peptide exhibited cytotoxicity against BEL-7402 human hepatocellular carcinoma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, U251 human glioma cells and MCF-7 human breast carcinoma cells. Additionally, SBP exhibited low cytotoxicity against HFL1 human normal fibroblast lung cells. The result suggested that the cytotoxic effect of the peptide is specific to tumor cells.

Show MeSH
Related in: MedlinePlus