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Foreign body response to subcutaneous implants in diabetic rats.

Socarrás TO, Vasconcelos AC, Campos PP, Pereira NB, Souza JP, Andrade SP - PLoS ONE (2014)

Bottom Line: Implantation of synthetic matrices and biomedical devices in diabetic individuals has become a common procedure to repair and/or replace biological tissues.Intra-implant levels of hemoglobin and vascular endothelial growth factor were not different after diabetes when compared with normoglycemic counterparts.All fibrogenic markers (transforming growth factor beta 1 levels, collagen deposition, fibrous capsule thickness, and foreign body giant cells) decreased after diabetes, whereas apoptosis (TUNEL) increased.

View Article: PubMed Central - PubMed

Affiliation: Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Department of Livestock Sciences, University of Córdoba, Montería, Córdoba, Colombia.

ABSTRACT
Implantation of synthetic matrices and biomedical devices in diabetic individuals has become a common procedure to repair and/or replace biological tissues. However, an adverse foreign body reaction that invariably occurs adjacent to implant devices impairing their function is poorly characterized in the diabetic environment. We investigated the influence of this condition on the abnormal tissue healing response in implants placed subcutaneously in normoglycemic and streptozotocin-induced diabetes in rats. In polyether-polyurethane sponge discs removed 10 days after implantation, the components of the fibrovascular tissue (angiogenesis, inflammation, fibrogenesis, and apoptosis) were assessed. Intra-implant levels of hemoglobin and vascular endothelial growth factor were not different after diabetes when compared with normoglycemic counterparts. However, there were a lower number of vessels in the fibrovascular tissue from diabetic rats when compared with vessel numbers in implants from non-diabetic animals. Overall, the inflammatory parameters (neutrophil accumulation--myeloperoxidase activity, tumor necrosis factor alpha, and monocyte chemotactic protein-1 levels and mast cell counting) increased in subcutaneous implants after diabetes induction. However, macrophage activation (N-acetyl-β-D-glucosaminidase activity) was lower in implants from diabetic rats when compared with those from normoglycemic animals. All fibrogenic markers (transforming growth factor beta 1 levels, collagen deposition, fibrous capsule thickness, and foreign body giant cells) decreased after diabetes, whereas apoptosis (TUNEL) increased. Our results showing that hyperglycemia down regulates the main features of the foreign body reaction induced by subcutaneous implants in rats may be relevant in understanding biomaterial integration and performance in diabetes.

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Markers of fibrogenesis in 10-day old implants from non-diabetic and diabetic rats.The amount of collagen (A) and levels of TGFβ1 (B) were increased in implants of non-diabetic rats as compared with that of diabetic rats. Representative histological sections (Picrossiurus-red staining) of implants from both groups of animals show distinct types of collagen in the implants (C) and (D). Values shown are expressed as mean±SEM. *Significant difference between non-diabetic and diabetic; p<0.05. Student's t-test. NSC, non-diabetic implant; DSC, diabetic implant; scale bar, 200 µm.
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pone-0110945-g004: Markers of fibrogenesis in 10-day old implants from non-diabetic and diabetic rats.The amount of collagen (A) and levels of TGFβ1 (B) were increased in implants of non-diabetic rats as compared with that of diabetic rats. Representative histological sections (Picrossiurus-red staining) of implants from both groups of animals show distinct types of collagen in the implants (C) and (D). Values shown are expressed as mean±SEM. *Significant difference between non-diabetic and diabetic; p<0.05. Student's t-test. NSC, non-diabetic implant; DSC, diabetic implant; scale bar, 200 µm.

Mentions: Fibrogenesis as an important wound healing and repair mechanism was measured by the cytokine TGF-β1 levels and total area of collagen (µm2) in the sponge implants (Picrossirius staining). A significant decrease in both parameters was observed in implants from diabetic when compared with that from non-diabetic rats (Fig. 4A–D).


Foreign body response to subcutaneous implants in diabetic rats.

Socarrás TO, Vasconcelos AC, Campos PP, Pereira NB, Souza JP, Andrade SP - PLoS ONE (2014)

Markers of fibrogenesis in 10-day old implants from non-diabetic and diabetic rats.The amount of collagen (A) and levels of TGFβ1 (B) were increased in implants of non-diabetic rats as compared with that of diabetic rats. Representative histological sections (Picrossiurus-red staining) of implants from both groups of animals show distinct types of collagen in the implants (C) and (D). Values shown are expressed as mean±SEM. *Significant difference between non-diabetic and diabetic; p<0.05. Student's t-test. NSC, non-diabetic implant; DSC, diabetic implant; scale bar, 200 µm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4220951&req=5

pone-0110945-g004: Markers of fibrogenesis in 10-day old implants from non-diabetic and diabetic rats.The amount of collagen (A) and levels of TGFβ1 (B) were increased in implants of non-diabetic rats as compared with that of diabetic rats. Representative histological sections (Picrossiurus-red staining) of implants from both groups of animals show distinct types of collagen in the implants (C) and (D). Values shown are expressed as mean±SEM. *Significant difference between non-diabetic and diabetic; p<0.05. Student's t-test. NSC, non-diabetic implant; DSC, diabetic implant; scale bar, 200 µm.
Mentions: Fibrogenesis as an important wound healing and repair mechanism was measured by the cytokine TGF-β1 levels and total area of collagen (µm2) in the sponge implants (Picrossirius staining). A significant decrease in both parameters was observed in implants from diabetic when compared with that from non-diabetic rats (Fig. 4A–D).

Bottom Line: Implantation of synthetic matrices and biomedical devices in diabetic individuals has become a common procedure to repair and/or replace biological tissues.Intra-implant levels of hemoglobin and vascular endothelial growth factor were not different after diabetes when compared with normoglycemic counterparts.All fibrogenic markers (transforming growth factor beta 1 levels, collagen deposition, fibrous capsule thickness, and foreign body giant cells) decreased after diabetes, whereas apoptosis (TUNEL) increased.

View Article: PubMed Central - PubMed

Affiliation: Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Department of Livestock Sciences, University of Córdoba, Montería, Córdoba, Colombia.

ABSTRACT
Implantation of synthetic matrices and biomedical devices in diabetic individuals has become a common procedure to repair and/or replace biological tissues. However, an adverse foreign body reaction that invariably occurs adjacent to implant devices impairing their function is poorly characterized in the diabetic environment. We investigated the influence of this condition on the abnormal tissue healing response in implants placed subcutaneously in normoglycemic and streptozotocin-induced diabetes in rats. In polyether-polyurethane sponge discs removed 10 days after implantation, the components of the fibrovascular tissue (angiogenesis, inflammation, fibrogenesis, and apoptosis) were assessed. Intra-implant levels of hemoglobin and vascular endothelial growth factor were not different after diabetes when compared with normoglycemic counterparts. However, there were a lower number of vessels in the fibrovascular tissue from diabetic rats when compared with vessel numbers in implants from non-diabetic animals. Overall, the inflammatory parameters (neutrophil accumulation--myeloperoxidase activity, tumor necrosis factor alpha, and monocyte chemotactic protein-1 levels and mast cell counting) increased in subcutaneous implants after diabetes induction. However, macrophage activation (N-acetyl-β-D-glucosaminidase activity) was lower in implants from diabetic rats when compared with those from normoglycemic animals. All fibrogenic markers (transforming growth factor beta 1 levels, collagen deposition, fibrous capsule thickness, and foreign body giant cells) decreased after diabetes, whereas apoptosis (TUNEL) increased. Our results showing that hyperglycemia down regulates the main features of the foreign body reaction induced by subcutaneous implants in rats may be relevant in understanding biomaterial integration and performance in diabetes.

Show MeSH
Related in: MedlinePlus