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Histone h4 modification during mouse spermatogenesis.

Shirakata Y, Hiradate Y, Inoue H, Sato E, Tanemura K - J. Reprod. Dev. (2014)

Bottom Line: The methylation pattern varied depending on the residues that were mono-, di-, or tri-methylated.All the H4 modifications were high during the meiotic prophase, suggesting that histone H4 modification plays an important role during this stage of spermatogenesis.Elongating spermatids showed increased acetylation of histone H4, which may be associated with a histone-to-protamine substitution.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.

ABSTRACT
The core histone is composed of four proteins (H2A, H2B, H3 and H4). Investigation of the modification patterns of histones is critical to understanding their roles in biological processes. Although histone modification is observed in multiple cells and tissues, little is known about its function in spermatogenesis. We focused on the modification patterns of histone H4 during murine spermatogenesis. We demonstrated that the individual N-terminal sites of H4 show different modification patterns during the differentiation of male germ cells. The methylation pattern varied depending on the residues that were mono-, di-, or tri-methylated. All the H4 modifications were high during the meiotic prophase, suggesting that histone H4 modification plays an important role during this stage of spermatogenesis. Elongating spermatids showed increased acetylation of histone H4, which may be associated with a histone-to-protamine substitution. Our results provide further insight into the specific relationship between histone H4 modification and gene expression during spermatogenesis, which could help to elucidate the epigenetic disorders underlying male infertility.

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Immunohistochemical analysis of histone H4 lysine 20 di-methylation and mono-methylation. The signals representnuclear (A, E, I, M, Q, U) histone H4 lysine 20 di-methylation (H4K20me2) (B, F, J, N, R, V) and histone H4mono-methylation (H4me) (C, G, K, O, S, W). Stage VIII seminiferous tubule is shown (A, B, C, D). A stage I (E, F,G, H), stage V (I, J, K, L), stage VIII (M, N, O, P), stage X (Q, R, S, T) and stage XII (U, V, W, X) seminiferoustubules are shown. The scale bars represent 50 µm (A, B, C, D) and 10 µm (H, L, P, T, X). P-SPC, pachytenespermatocyte; PL-SPC, preleptotene spermatocyte; L-SPC, leptotene spermatocyte; Z-SPC, zygotene spermatocyte; R-SPD,round spermatid; and E-SPD, elongated spermatid.
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fig_003: Immunohistochemical analysis of histone H4 lysine 20 di-methylation and mono-methylation. The signals representnuclear (A, E, I, M, Q, U) histone H4 lysine 20 di-methylation (H4K20me2) (B, F, J, N, R, V) and histone H4mono-methylation (H4me) (C, G, K, O, S, W). Stage VIII seminiferous tubule is shown (A, B, C, D). A stage I (E, F,G, H), stage V (I, J, K, L), stage VIII (M, N, O, P), stage X (Q, R, S, T) and stage XII (U, V, W, X) seminiferoustubules are shown. The scale bars represent 50 µm (A, B, C, D) and 10 µm (H, L, P, T, X). P-SPC, pachytenespermatocyte; PL-SPC, preleptotene spermatocyte; L-SPC, leptotene spermatocyte; Z-SPC, zygotene spermatocyte; R-SPD,round spermatid; and E-SPD, elongated spermatid.

Mentions: H4K20me2 varied dynamically during spermatogenesis [25]. Its staining was moderate inspermatogonia, high at the preleptotene spermatocyte stage and similar in leptotene and zygotene spermatocytes (Fig. 3N, R, VFig. 3.


Histone h4 modification during mouse spermatogenesis.

Shirakata Y, Hiradate Y, Inoue H, Sato E, Tanemura K - J. Reprod. Dev. (2014)

Immunohistochemical analysis of histone H4 lysine 20 di-methylation and mono-methylation. The signals representnuclear (A, E, I, M, Q, U) histone H4 lysine 20 di-methylation (H4K20me2) (B, F, J, N, R, V) and histone H4mono-methylation (H4me) (C, G, K, O, S, W). Stage VIII seminiferous tubule is shown (A, B, C, D). A stage I (E, F,G, H), stage V (I, J, K, L), stage VIII (M, N, O, P), stage X (Q, R, S, T) and stage XII (U, V, W, X) seminiferoustubules are shown. The scale bars represent 50 µm (A, B, C, D) and 10 µm (H, L, P, T, X). P-SPC, pachytenespermatocyte; PL-SPC, preleptotene spermatocyte; L-SPC, leptotene spermatocyte; Z-SPC, zygotene spermatocyte; R-SPD,round spermatid; and E-SPD, elongated spermatid.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219996&req=5

fig_003: Immunohistochemical analysis of histone H4 lysine 20 di-methylation and mono-methylation. The signals representnuclear (A, E, I, M, Q, U) histone H4 lysine 20 di-methylation (H4K20me2) (B, F, J, N, R, V) and histone H4mono-methylation (H4me) (C, G, K, O, S, W). Stage VIII seminiferous tubule is shown (A, B, C, D). A stage I (E, F,G, H), stage V (I, J, K, L), stage VIII (M, N, O, P), stage X (Q, R, S, T) and stage XII (U, V, W, X) seminiferoustubules are shown. The scale bars represent 50 µm (A, B, C, D) and 10 µm (H, L, P, T, X). P-SPC, pachytenespermatocyte; PL-SPC, preleptotene spermatocyte; L-SPC, leptotene spermatocyte; Z-SPC, zygotene spermatocyte; R-SPD,round spermatid; and E-SPD, elongated spermatid.
Mentions: H4K20me2 varied dynamically during spermatogenesis [25]. Its staining was moderate inspermatogonia, high at the preleptotene spermatocyte stage and similar in leptotene and zygotene spermatocytes (Fig. 3N, R, VFig. 3.

Bottom Line: The methylation pattern varied depending on the residues that were mono-, di-, or tri-methylated.All the H4 modifications were high during the meiotic prophase, suggesting that histone H4 modification plays an important role during this stage of spermatogenesis.Elongating spermatids showed increased acetylation of histone H4, which may be associated with a histone-to-protamine substitution.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.

ABSTRACT
The core histone is composed of four proteins (H2A, H2B, H3 and H4). Investigation of the modification patterns of histones is critical to understanding their roles in biological processes. Although histone modification is observed in multiple cells and tissues, little is known about its function in spermatogenesis. We focused on the modification patterns of histone H4 during murine spermatogenesis. We demonstrated that the individual N-terminal sites of H4 show different modification patterns during the differentiation of male germ cells. The methylation pattern varied depending on the residues that were mono-, di-, or tri-methylated. All the H4 modifications were high during the meiotic prophase, suggesting that histone H4 modification plays an important role during this stage of spermatogenesis. Elongating spermatids showed increased acetylation of histone H4, which may be associated with a histone-to-protamine substitution. Our results provide further insight into the specific relationship between histone H4 modification and gene expression during spermatogenesis, which could help to elucidate the epigenetic disorders underlying male infertility.

Show MeSH
Related in: MedlinePlus