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Epigenetic modification agents improve genomic methylation reprogramming in porcine cloned embryos.

Huan YJ, Zhu J, Wang HM, Wu ZF, Zhang JG, Xie BT, Li JY, Kong QR, Liu ZH, He HB - J. Reprod. Dev. (2014)

Bottom Line: When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts.Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos.In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.

View Article: PubMed Central - PubMed

Affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

ABSTRACT
Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.

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Transcript levels of early embryo development-related genes at the zygotic genome activation and blastocyst stagesof IVF, NT-CON, NT-AZA and NT-TSA embryos. The transcript abundance for each gene (Thy1 and Col5a2 in clonedembryos) in IVF embryos was considered the control. The data are expressed as means ± SEM. a–c Values fora given gene at a certain stage in columns with different superscripts differ significantly (P < 0.05).
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fig_002: Transcript levels of early embryo development-related genes at the zygotic genome activation and blastocyst stagesof IVF, NT-CON, NT-AZA and NT-TSA embryos. The transcript abundance for each gene (Thy1 and Col5a2 in clonedembryos) in IVF embryos was considered the control. The data are expressed as means ± SEM. a–c Values fora given gene at a certain stage in columns with different superscripts differ significantly (P < 0.05).

Mentions: To further explore the mechanism underlying the improved development of cloned embryos treated with 5-aza-dC or TSA, thetranscript levels of early embryonic development-related genes in the zygotic genome activation (ZGA) andblastocyst stages were investigated (Fig. 2Fig. 2.


Epigenetic modification agents improve genomic methylation reprogramming in porcine cloned embryos.

Huan YJ, Zhu J, Wang HM, Wu ZF, Zhang JG, Xie BT, Li JY, Kong QR, Liu ZH, He HB - J. Reprod. Dev. (2014)

Transcript levels of early embryo development-related genes at the zygotic genome activation and blastocyst stagesof IVF, NT-CON, NT-AZA and NT-TSA embryos. The transcript abundance for each gene (Thy1 and Col5a2 in clonedembryos) in IVF embryos was considered the control. The data are expressed as means ± SEM. a–c Values fora given gene at a certain stage in columns with different superscripts differ significantly (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219995&req=5

fig_002: Transcript levels of early embryo development-related genes at the zygotic genome activation and blastocyst stagesof IVF, NT-CON, NT-AZA and NT-TSA embryos. The transcript abundance for each gene (Thy1 and Col5a2 in clonedembryos) in IVF embryos was considered the control. The data are expressed as means ± SEM. a–c Values fora given gene at a certain stage in columns with different superscripts differ significantly (P < 0.05).
Mentions: To further explore the mechanism underlying the improved development of cloned embryos treated with 5-aza-dC or TSA, thetranscript levels of early embryonic development-related genes in the zygotic genome activation (ZGA) andblastocyst stages were investigated (Fig. 2Fig. 2.

Bottom Line: When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts.Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos.In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.

View Article: PubMed Central - PubMed

Affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

ABSTRACT
Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.

Show MeSH
Related in: MedlinePlus