Limits...
Lactobacillus acidophilus L-92 Cells Activate Expression of Immunomodulatory Genes in THP-1 Cells.

Yanagihara S, Goto H, Hirota T, Fukuda S, Ohno H, Yamamoto N - Biosci Microbiota Food Health (2014)

Bottom Line: Two transmembrane receptor genes, TLR7 and ICAM1, were also upregulated in the early phase of treatment.Some genes encoding cytokines, such as IL1A, IL1B and IL8, and the enzyme IDO1 were upregulated at both the early and the late phases of treatment.These results suggest that probiotic L-92 might promote Th1 and regulatory T-cell responses by activation of the MAPK signaling pathway, followed by the NOD-like receptor signaling pathway in THP-1 cells.

View Article: PubMed Central - PubMed

Affiliation: Microbiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.

ABSTRACT
To understand the immunomodulatory effects of Lactobacillus acidophilus L-92 cells suggested from our previous study of in vivo anti-allergy and anti-virus effects, host immune responses in macrophage-like THP-1 cells after 4 h (the early phase) and 24 h (the late phase) of cocultivation with L-92 cells were investigated by transcriptome analysis. In the early phase of L-92 treatment, various transcription regulator genes, such as, NFkB1, NFkB2, JUN, HIVEP2 and RELB, and genes encoding chemokines and cytokines, such as CCL4, CXCL11, CCL3 and TNF, were upregulated. Two transmembrane receptor genes, TLR7 and ICAM1, were also upregulated in the early phase of treatment. In contrast, many transmembrane receptor genes, such as IL7R, CD80, CRLF2, CD86, CD5, HLA-DQA1, IL2RA, IL15RA and CSF2RA, and some cytokine genes, including IL6, IL23A and CCL22, were significantly upregulated in the late phase after L-92 exposure. Some genes encoding cytokines, such as IL1A, IL1B and IL8, and the enzyme IDO1 were upregulated at both the early and the late phases of treatment. These results suggest that probiotic L-92 might promote Th1 and regulatory T-cell responses by activation of the MAPK signaling pathway, followed by the NOD-like receptor signaling pathway in THP-1 cells.

No MeSH data available.


Related in: MedlinePlus

Changes in gene expression in THP-1 cells after 4 hr of treatment with Lactobacillus acidophilus L-92cells. Genes that were significantly upregulated by L-92 treatment were categorized by the IPA method as described in theMaterials and Methods. Upregulated genes are indicated by red arrows. Black thin arrows represent the predicted flow of thepathway. Genes grouped by KEGG analysis are indicated by the red dotted line.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4219982&req=5

fig_002: Changes in gene expression in THP-1 cells after 4 hr of treatment with Lactobacillus acidophilus L-92cells. Genes that were significantly upregulated by L-92 treatment were categorized by the IPA method as described in theMaterials and Methods. Upregulated genes are indicated by red arrows. Black thin arrows represent the predicted flow of thepathway. Genes grouped by KEGG analysis are indicated by the red dotted line.

Mentions: These cell responses at 4 hr and 24 hr are illustrated in Fig. 2Fig. 2.


Lactobacillus acidophilus L-92 Cells Activate Expression of Immunomodulatory Genes in THP-1 Cells.

Yanagihara S, Goto H, Hirota T, Fukuda S, Ohno H, Yamamoto N - Biosci Microbiota Food Health (2014)

Changes in gene expression in THP-1 cells after 4 hr of treatment with Lactobacillus acidophilus L-92cells. Genes that were significantly upregulated by L-92 treatment were categorized by the IPA method as described in theMaterials and Methods. Upregulated genes are indicated by red arrows. Black thin arrows represent the predicted flow of thepathway. Genes grouped by KEGG analysis are indicated by the red dotted line.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219982&req=5

fig_002: Changes in gene expression in THP-1 cells after 4 hr of treatment with Lactobacillus acidophilus L-92cells. Genes that were significantly upregulated by L-92 treatment were categorized by the IPA method as described in theMaterials and Methods. Upregulated genes are indicated by red arrows. Black thin arrows represent the predicted flow of thepathway. Genes grouped by KEGG analysis are indicated by the red dotted line.
Mentions: These cell responses at 4 hr and 24 hr are illustrated in Fig. 2Fig. 2.

Bottom Line: Two transmembrane receptor genes, TLR7 and ICAM1, were also upregulated in the early phase of treatment.Some genes encoding cytokines, such as IL1A, IL1B and IL8, and the enzyme IDO1 were upregulated at both the early and the late phases of treatment.These results suggest that probiotic L-92 might promote Th1 and regulatory T-cell responses by activation of the MAPK signaling pathway, followed by the NOD-like receptor signaling pathway in THP-1 cells.

View Article: PubMed Central - PubMed

Affiliation: Microbiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.

ABSTRACT
To understand the immunomodulatory effects of Lactobacillus acidophilus L-92 cells suggested from our previous study of in vivo anti-allergy and anti-virus effects, host immune responses in macrophage-like THP-1 cells after 4 h (the early phase) and 24 h (the late phase) of cocultivation with L-92 cells were investigated by transcriptome analysis. In the early phase of L-92 treatment, various transcription regulator genes, such as, NFkB1, NFkB2, JUN, HIVEP2 and RELB, and genes encoding chemokines and cytokines, such as CCL4, CXCL11, CCL3 and TNF, were upregulated. Two transmembrane receptor genes, TLR7 and ICAM1, were also upregulated in the early phase of treatment. In contrast, many transmembrane receptor genes, such as IL7R, CD80, CRLF2, CD86, CD5, HLA-DQA1, IL2RA, IL15RA and CSF2RA, and some cytokine genes, including IL6, IL23A and CCL22, were significantly upregulated in the late phase after L-92 exposure. Some genes encoding cytokines, such as IL1A, IL1B and IL8, and the enzyme IDO1 were upregulated at both the early and the late phases of treatment. These results suggest that probiotic L-92 might promote Th1 and regulatory T-cell responses by activation of the MAPK signaling pathway, followed by the NOD-like receptor signaling pathway in THP-1 cells.

No MeSH data available.


Related in: MedlinePlus