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Comparison of the Growth of Lactobacillus delbrueckii, L. paracasei and L. plantarum on Inulin in Co-culture Systems.

Takagi R, Tsujikawa Y, Nomoto R, Osawa R - Biosci Microbiota Food Health (2014)

Bottom Line: These results suggest that L. delbrueckii TU-1 and L. paracasei KTN-5 were efficient at utilizing inulin and fructose, respectively.The growth of L. delbrueckii TU-1 was unaffected but that of L. paracasei KTN-5 was markedly suppressed.This evidence suggests that prebiotic use of inulin supported the selective growth of intracellular inulin degraders such as L. delbrueckii rather than extracellular inulin degraders such as L. paracasei in the host microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokko-dai, Nada-ku, Kobe 657-8501, Japan.

ABSTRACT
Lactobacillus delbrueckii TU-1, which apparently takes intact inulin into its cells and then degrades it intracellularly, was co-cultured in vitro with L. paracasei KTN-5, an extracellular inulin degrader; or L. plantarum 22A-3, a strain that is able to utilize fructose but not inulin; or both in order to prequalify inulin as a prebiotic agent in vivo. When L. delbrueckii TU-1 was co-cultured with L. paracasei KTN-5 on fructose or inulin, the growth of L. delbrueckii TU-1 on inulin was markedly higher than that of L. paracasei KTN-5, whereas the growth of L. delbrueckii TU-1 on fructose was much lower than that of L. paracasei KTN-5. These results suggest that L. delbrueckii TU-1 and L. paracasei KTN-5 were efficient at utilizing inulin and fructose, respectively. When L. plantarum 22A-3 was co-cultured with L. delbrueckii TU-1 on inulin, the growth of L. plantarum 22A-3 was enhanced by L. paracasei KTN-5 but not by L. delbrueckii TU-1, suggesting that the fructose moiety that L. paracasei KTN-5 released temporarily into the medium was "scavenged" by L. plantarum 22A-3. Thus, L. delbrueckii TU-1, L. paracasei KTN-5, and L. plantarum 22A-3 were then cultured altogether on inulin. The growth of L. delbrueckii TU-1 was unaffected but that of L. paracasei KTN-5 was markedly suppressed. This evidence suggests that prebiotic use of inulin supported the selective growth of intracellular inulin degraders such as L. delbrueckii rather than extracellular inulin degraders such as L. paracasei in the host microbiota.

No MeSH data available.


Related in: MedlinePlus

Growth curve of a co-culture of L. delbrueckii TU-1, L. paracasei KTN-5, and L.plantarum 22A-3 in the‚ÄÖmMRS medium with a 2% (wt/vol) concentration of inulin.‚óŹ, Growth of L. delbrueckii TU-1; ‚Ė°, growth of L. paracasei KTN-5; ‚Ė≤, growth ofL. plantarum 22A-3. Each experiment was performed in triplicate.
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fig_005: Growth curve of a co-culture of L. delbrueckii TU-1, L. paracasei KTN-5, and L.plantarum 22A-3 in the‚ÄÖmMRS medium with a 2% (wt/vol) concentration of inulin.‚óŹ, Growth of L. delbrueckii TU-1; ‚Ė°, growth of L. paracasei KTN-5; ‚Ė≤, growth ofL. plantarum 22A-3. Each experiment was performed in triplicate.

Mentions: In the triple co-culture of L. delbrueckii TU-1, L. paracasei KTN-5, andL. plantarum 22A-3 on inulin (Fig. 5Fig. 5.


Comparison of the Growth of Lactobacillus delbrueckii, L. paracasei and L. plantarum on Inulin in Co-culture Systems.

Takagi R, Tsujikawa Y, Nomoto R, Osawa R - Biosci Microbiota Food Health (2014)

Growth curve of a co-culture of L. delbrueckii TU-1, L. paracasei KTN-5, and L.plantarum 22A-3 in the‚ÄÖmMRS medium with a 2% (wt/vol) concentration of inulin.‚óŹ, Growth of L. delbrueckii TU-1; ‚Ė°, growth of L. paracasei KTN-5; ‚Ė≤, growth ofL. plantarum 22A-3. Each experiment was performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219980&req=5

fig_005: Growth curve of a co-culture of L. delbrueckii TU-1, L. paracasei KTN-5, and L.plantarum 22A-3 in the‚ÄÖmMRS medium with a 2% (wt/vol) concentration of inulin.‚óŹ, Growth of L. delbrueckii TU-1; ‚Ė°, growth of L. paracasei KTN-5; ‚Ė≤, growth ofL. plantarum 22A-3. Each experiment was performed in triplicate.
Mentions: In the triple co-culture of L. delbrueckii TU-1, L. paracasei KTN-5, andL. plantarum 22A-3 on inulin (Fig. 5Fig. 5.

Bottom Line: These results suggest that L. delbrueckii TU-1 and L. paracasei KTN-5 were efficient at utilizing inulin and fructose, respectively.The growth of L. delbrueckii TU-1 was unaffected but that of L. paracasei KTN-5 was markedly suppressed.This evidence suggests that prebiotic use of inulin supported the selective growth of intracellular inulin degraders such as L. delbrueckii rather than extracellular inulin degraders such as L. paracasei in the host microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokko-dai, Nada-ku, Kobe 657-8501, Japan.

ABSTRACT
Lactobacillus delbrueckii TU-1, which apparently takes intact inulin into its cells and then degrades it intracellularly, was co-cultured in vitro with L. paracasei KTN-5, an extracellular inulin degrader; or L. plantarum 22A-3, a strain that is able to utilize fructose but not inulin; or both in order to prequalify inulin as a prebiotic agent in vivo. When L. delbrueckii TU-1 was co-cultured with L. paracasei KTN-5 on fructose or inulin, the growth of L. delbrueckii TU-1 on inulin was markedly higher than that of L. paracasei KTN-5, whereas the growth of L. delbrueckii TU-1 on fructose was much lower than that of L. paracasei KTN-5. These results suggest that L. delbrueckii TU-1 and L. paracasei KTN-5 were efficient at utilizing inulin and fructose, respectively. When L. plantarum 22A-3 was co-cultured with L. delbrueckii TU-1 on inulin, the growth of L. plantarum 22A-3 was enhanced by L. paracasei KTN-5 but not by L. delbrueckii TU-1, suggesting that the fructose moiety that L. paracasei KTN-5 released temporarily into the medium was "scavenged" by L. plantarum 22A-3. Thus, L. delbrueckii TU-1, L. paracasei KTN-5, and L. plantarum 22A-3 were then cultured altogether on inulin. The growth of L. delbrueckii TU-1 was unaffected but that of L. paracasei KTN-5 was markedly suppressed. This evidence suggests that prebiotic use of inulin supported the selective growth of intracellular inulin degraders such as L. delbrueckii rather than extracellular inulin degraders such as L. paracasei in the host microbiota.

No MeSH data available.


Related in: MedlinePlus