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Pilot Study on Interferon-γ-producing T Cell Subsets after the Protective Vaccination with Radiation-attenuated Cercaria of Schistosoma japonicum in the Miniature Pig Model.

Abdel-Hafeez EH, Watanabe K, Kamei K, Kikuchi M, Chen H, Daniel B, Yu C, Hirayama K - Trop Med Health (2014)

Bottom Line: To characterize the protective immune response of the pig model vaccinated by attenuated cercaria, flow cytometric analysis of the reactive T cell subsets was performed.CD4+ αβ-T cells as well as CD4+/CD8α(mid) double positive and/or CD8α(high) αβ-T cells were the major IFN-γ-producing CD3+ T cells.On the contrary, γδ T cells did not produce intracellular IFN-γ.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University , 1-14-2 Sakamoto, Nagasaki 852-8523, Japan ; Department of Parasitology, Faculty of Medicine, Minia University , Minia, 61519, Egypt.

ABSTRACT
CLAWN miniature pig has been shown to serve as a suitable host for the experimental infection of Schistosoma japonicum. In this study, we found that radiation-attenuated cercaria (RAC) vaccine gave CLAWN miniature pigs protective immunity against subsequent challenge infection with S. japonicum cercaria. To characterize the protective immune response of the pig model vaccinated by attenuated cercaria, flow cytometric analysis of the reactive T cell subsets was performed. The intracellular interferon (IFN)-γ and the cell surface markers revealed the peripheral blood CD3+ T-lymphocytes produced significant amounts of IFN-γ during the immunization period and after the challenge infection. CD4+ αβ-T cells as well as CD4+/CD8α(mid) double positive and/or CD8α(high) αβ-T cells were the major IFN-γ-producing CD3+ T cells. On the contrary, γδ T cells did not produce intracellular IFN-γ. Our results suggested that RAC-vaccinated miniature pigs showed effective protective immunity through the activation of αβ T cells bearing antigen specific T-cell receptors but not through the activation of γδ T cells.

No MeSH data available.


Related in: MedlinePlus

Cytokine production by PBL in RAC-immunized miniature pigsPBMC were cultured with PHA for 4 days and cytokine levels measured using ELISA. (a) IFN-γ, (b) IL-4 and (c) IL-10 levels. Open columns show the data from the control group. Shaded columns show the data from the immunized group. Data are presented as the mean ± SEM.
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Figure 2: Cytokine production by PBL in RAC-immunized miniature pigsPBMC were cultured with PHA for 4 days and cytokine levels measured using ELISA. (a) IFN-γ, (b) IL-4 and (c) IL-10 levels. Open columns show the data from the control group. Shaded columns show the data from the immunized group. Data are presented as the mean ± SEM.

Mentions: We also evaluated cytokine response using ELISA (Fig. 2). In order to achieve this, peripheral blood was collected and the PBMC were isolated. PHA was added to PBMC for 2 days to stimulate IL-4 levels and 4 days to stimulate IFN-γ and IL-10. Cytokines in the culture supernatant were then measured by ELISA. The RAC-immunized miniature pigs produced more IFN-γ and IL-4 than the control miniature pigs during the immunization period, although the differences were not statistically significant due to the small number of pigs. IFN-γ production was found to peak 3 weeks after infection. The amount of IFN-γ was greater than that of IL-4 produced in the RAC-immunized miniature pig PBMC. Unlike IFN-γ production, IL-4 production peaked at 1 week after infection. Throughout the course of immunization and infection, the immunized miniature pig PBMC produced significantly higher levels of IL-10 than the control miniature pigs.


Pilot Study on Interferon-γ-producing T Cell Subsets after the Protective Vaccination with Radiation-attenuated Cercaria of Schistosoma japonicum in the Miniature Pig Model.

Abdel-Hafeez EH, Watanabe K, Kamei K, Kikuchi M, Chen H, Daniel B, Yu C, Hirayama K - Trop Med Health (2014)

Cytokine production by PBL in RAC-immunized miniature pigsPBMC were cultured with PHA for 4 days and cytokine levels measured using ELISA. (a) IFN-γ, (b) IL-4 and (c) IL-10 levels. Open columns show the data from the control group. Shaded columns show the data from the immunized group. Data are presented as the mean ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4219940&req=5

Figure 2: Cytokine production by PBL in RAC-immunized miniature pigsPBMC were cultured with PHA for 4 days and cytokine levels measured using ELISA. (a) IFN-γ, (b) IL-4 and (c) IL-10 levels. Open columns show the data from the control group. Shaded columns show the data from the immunized group. Data are presented as the mean ± SEM.
Mentions: We also evaluated cytokine response using ELISA (Fig. 2). In order to achieve this, peripheral blood was collected and the PBMC were isolated. PHA was added to PBMC for 2 days to stimulate IL-4 levels and 4 days to stimulate IFN-γ and IL-10. Cytokines in the culture supernatant were then measured by ELISA. The RAC-immunized miniature pigs produced more IFN-γ and IL-4 than the control miniature pigs during the immunization period, although the differences were not statistically significant due to the small number of pigs. IFN-γ production was found to peak 3 weeks after infection. The amount of IFN-γ was greater than that of IL-4 produced in the RAC-immunized miniature pig PBMC. Unlike IFN-γ production, IL-4 production peaked at 1 week after infection. Throughout the course of immunization and infection, the immunized miniature pig PBMC produced significantly higher levels of IL-10 than the control miniature pigs.

Bottom Line: To characterize the protective immune response of the pig model vaccinated by attenuated cercaria, flow cytometric analysis of the reactive T cell subsets was performed.CD4+ αβ-T cells as well as CD4+/CD8α(mid) double positive and/or CD8α(high) αβ-T cells were the major IFN-γ-producing CD3+ T cells.On the contrary, γδ T cells did not produce intracellular IFN-γ.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University , 1-14-2 Sakamoto, Nagasaki 852-8523, Japan ; Department of Parasitology, Faculty of Medicine, Minia University , Minia, 61519, Egypt.

ABSTRACT
CLAWN miniature pig has been shown to serve as a suitable host for the experimental infection of Schistosoma japonicum. In this study, we found that radiation-attenuated cercaria (RAC) vaccine gave CLAWN miniature pigs protective immunity against subsequent challenge infection with S. japonicum cercaria. To characterize the protective immune response of the pig model vaccinated by attenuated cercaria, flow cytometric analysis of the reactive T cell subsets was performed. The intracellular interferon (IFN)-γ and the cell surface markers revealed the peripheral blood CD3+ T-lymphocytes produced significant amounts of IFN-γ during the immunization period and after the challenge infection. CD4+ αβ-T cells as well as CD4+/CD8α(mid) double positive and/or CD8α(high) αβ-T cells were the major IFN-γ-producing CD3+ T cells. On the contrary, γδ T cells did not produce intracellular IFN-γ. Our results suggested that RAC-vaccinated miniature pigs showed effective protective immunity through the activation of αβ T cells bearing antigen specific T-cell receptors but not through the activation of γδ T cells.

No MeSH data available.


Related in: MedlinePlus