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Combined therapeutic effects of low power laser (980nm) and CoQ10 on Neuropathic Pain in adult male rat.

Jameie SB, Masoumipoor M, Janzadeh A, Nasirinezhad F, Kerdari M, Soleimani M - Med J Islam Repub Iran (2014)

Bottom Line: As we expected CCI decreased the pain threshold, whereas CoQ10 administration for two weeks increased mechanical and thermal threshold.The same results obtained for laser therapy using the CCI animals.Combination of laser 980nm with CoQ10 also showed significant differences in CCI animals.

View Article: PubMed Central - HTML - PubMed

Affiliation: 1. Associate Professor of Anatomy and Neuroscience, Department of Medical Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. jameie.sb@iums.ac.ir & behjame@gmail.com.

ABSTRACT

Background: Neuropathic pain (NP) is one of the most suffering medical conditions that often fail to respond to certain pain therapy. Although its exact etiology is still unknown the role of reactive oxygen species (ROS) and oxidative stress were explored by many researchers. Neuropathies either central or peripheral lead to painful condition as well as social and economic isolation, thus various therapies were used to treat or reduce the pain. Laser therapy and antioxidant drugs have separately considered as treatment for NP, but the combination of them have not been used yet. In order to study the combination effects of Low Level Laser Therapy (LLLT) and Coenzyme Q10 (CoQ10) the present study was designed.

Methods: Sixty adult male rats (230-320g) were used in this experimental study that divided into six groups (n=10). Chronic constriction injury (CCI) was used to induce neuropathic pain. The CoQ10 or vehicle, a low level laser of 980nm was used for two consecutive weeks. Thermal and mechanical paw withdrawal thresholds were assessed before and after surgery on 7(th) and 14(th) days.

Results: As we expected CCI decreased the pain threshold, whereas CoQ10 administration for two weeks increased mechanical and thermal threshold. The same results obtained for laser therapy using the CCI animals. Combination of laser 980nm with CoQ10 also showed significant differences in CCI animals.

Conclusion: Based on our findings the combination of CoQ10 with LLLT showed better effects than each one alone. In this regard we believe that there might be cellular and molecular synergism in simultaneous use of CoQ10 and LLLT on pain relief.

No MeSH data available.


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Mentions: The mean of mechanical withdrawal threshold of the control group was 19.18±4.66 g before surgery. The means for the CCI group were 10.17±4.18 g and 9.15±4.20 g on the 7th and the 14th day respectively after surgery. For the CoQ10 group, the mean values were 15.29±5.50 g and 13.19±4.12 g on the 7th and the 14th day after surgery. For the Vehicle group, the mean values were 12.09±3.92 sec and 9.75±3.15 sec on the 7th and the 14th day respectively after surgery. For the LLLT 980nm group, the mean values were 14.15±4.25 g and 12.35±5.28 g on the 7th day and the 14th day respectively after surgery. For the LLLT 980nm+CoQ10 group, the mean values were 14.65±5.77 sec and 12.72±4.63 sec on the 7th and the 14th day respectively after surgery. For the LLLT 980nm+Vehicle group, the mean values were 13.6±3.50 sec and 10.98±2.99 sec on the 7th and the 14th day respectively after surgery. Statistical analysis indicated that the difference between control group and the CCI group on the 7th, 14th post-surgery days, was significant at (p< 0.001); also difference between CoQ10 group and the CCI group on the 7th, 14th post-surgery days, was significant (p< 0.001, p< 0.01); and difference between LLLT 980nm group and the CCI group on the 7th, 14th post-surgery days, was also significant (p<0.01, p<0.05). Moreover, difference between LLLT 980nm+CoQ10 group and the CCI group on the 7th, 14th post-surgery days, was significant (p< 0.001, p< 0.05). Difference between LLLT 980nm+Vehicle group and the CCI group on the 7thpost-surgery day was significant (p< 0.01) but there was no significant difference on 14th day post-surgery. Also, there was significant difference between the 7th, 14th post-surgery days of the treatment groups and the control group (p< 0.001). In a comparison between the LLLT 980nm+CoQ10 and LLLT 980nm+Vehicle groups, there were no significant differences in the values on the 7th, 14th post-surgery days and comparison between the LLLT 980nm+CoQ10 group and treatment groups (CoQ10 and LLLT 980nm), showed no significant difference on the 7th, 14th days (Fig 3 & Fig 4).


Combined therapeutic effects of low power laser (980nm) and CoQ10 on Neuropathic Pain in adult male rat.

Jameie SB, Masoumipoor M, Janzadeh A, Nasirinezhad F, Kerdari M, Soleimani M - Med J Islam Repub Iran (2014)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219887&req=5

Mentions: The mean of mechanical withdrawal threshold of the control group was 19.18±4.66 g before surgery. The means for the CCI group were 10.17±4.18 g and 9.15±4.20 g on the 7th and the 14th day respectively after surgery. For the CoQ10 group, the mean values were 15.29±5.50 g and 13.19±4.12 g on the 7th and the 14th day after surgery. For the Vehicle group, the mean values were 12.09±3.92 sec and 9.75±3.15 sec on the 7th and the 14th day respectively after surgery. For the LLLT 980nm group, the mean values were 14.15±4.25 g and 12.35±5.28 g on the 7th day and the 14th day respectively after surgery. For the LLLT 980nm+CoQ10 group, the mean values were 14.65±5.77 sec and 12.72±4.63 sec on the 7th and the 14th day respectively after surgery. For the LLLT 980nm+Vehicle group, the mean values were 13.6±3.50 sec and 10.98±2.99 sec on the 7th and the 14th day respectively after surgery. Statistical analysis indicated that the difference between control group and the CCI group on the 7th, 14th post-surgery days, was significant at (p< 0.001); also difference between CoQ10 group and the CCI group on the 7th, 14th post-surgery days, was significant (p< 0.001, p< 0.01); and difference between LLLT 980nm group and the CCI group on the 7th, 14th post-surgery days, was also significant (p<0.01, p<0.05). Moreover, difference between LLLT 980nm+CoQ10 group and the CCI group on the 7th, 14th post-surgery days, was significant (p< 0.001, p< 0.05). Difference between LLLT 980nm+Vehicle group and the CCI group on the 7thpost-surgery day was significant (p< 0.01) but there was no significant difference on 14th day post-surgery. Also, there was significant difference between the 7th, 14th post-surgery days of the treatment groups and the control group (p< 0.001). In a comparison between the LLLT 980nm+CoQ10 and LLLT 980nm+Vehicle groups, there were no significant differences in the values on the 7th, 14th post-surgery days and comparison between the LLLT 980nm+CoQ10 group and treatment groups (CoQ10 and LLLT 980nm), showed no significant difference on the 7th, 14th days (Fig 3 & Fig 4).

Bottom Line: As we expected CCI decreased the pain threshold, whereas CoQ10 administration for two weeks increased mechanical and thermal threshold.The same results obtained for laser therapy using the CCI animals.Combination of laser 980nm with CoQ10 also showed significant differences in CCI animals.

View Article: PubMed Central - HTML - PubMed

Affiliation: 1. Associate Professor of Anatomy and Neuroscience, Department of Medical Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. jameie.sb@iums.ac.ir & behjame@gmail.com.

ABSTRACT

Background: Neuropathic pain (NP) is one of the most suffering medical conditions that often fail to respond to certain pain therapy. Although its exact etiology is still unknown the role of reactive oxygen species (ROS) and oxidative stress were explored by many researchers. Neuropathies either central or peripheral lead to painful condition as well as social and economic isolation, thus various therapies were used to treat or reduce the pain. Laser therapy and antioxidant drugs have separately considered as treatment for NP, but the combination of them have not been used yet. In order to study the combination effects of Low Level Laser Therapy (LLLT) and Coenzyme Q10 (CoQ10) the present study was designed.

Methods: Sixty adult male rats (230-320g) were used in this experimental study that divided into six groups (n=10). Chronic constriction injury (CCI) was used to induce neuropathic pain. The CoQ10 or vehicle, a low level laser of 980nm was used for two consecutive weeks. Thermal and mechanical paw withdrawal thresholds were assessed before and after surgery on 7(th) and 14(th) days.

Results: As we expected CCI decreased the pain threshold, whereas CoQ10 administration for two weeks increased mechanical and thermal threshold. The same results obtained for laser therapy using the CCI animals. Combination of laser 980nm with CoQ10 also showed significant differences in CCI animals.

Conclusion: Based on our findings the combination of CoQ10 with LLLT showed better effects than each one alone. In this regard we believe that there might be cellular and molecular synergism in simultaneous use of CoQ10 and LLLT on pain relief.

No MeSH data available.


Related in: MedlinePlus