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L-histidine inhibits biofilm formation and FLO11-associated phenotypes in Saccharomyces cerevisiae flor yeasts.

Bou Zeidan M, Zara G, Viti C, Decorosi F, Mannazzu I, Budroni M, Giovannetti L, Zara S - PLoS ONE (2014)

Bottom Line: L-histidine completely inhibited growth and its effect on viability was inversely related to Flo11p expression.Accordingly, L-histidine did not affect the viability of the Δflo11 and S288c strains.Also, L-histidine dramatically decreased air-liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of the Flo11p gene.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Agraria, University of Sassari, Sassari, Italy.

ABSTRACT
Flor yeasts of Saccharomyces cerevisiae have an innate diversity of Flo11p which codes for a highly hydrophobic and anionic cell-wall glycoprotein with a fundamental role in biofilm formation. In this study, 380 nitrogen compounds were administered to three S. cerevisiae flor strains handling Flo11p alleles with different expression levels. S. cerevisiae strain S288c was used as the reference strain as it cannot produce Flo11p. The flor strains generally metabolized amino acids and dipeptides as the sole nitrogen source, although with some exceptions regarding L-histidine and histidine containing dipeptides. L-histidine completely inhibited growth and its effect on viability was inversely related to Flo11p expression. Accordingly, L-histidine did not affect the viability of the Δflo11 and S288c strains. Also, L-histidine dramatically decreased air-liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of the Flo11p gene. Moreover, L-histidine modified the chitin and glycans content on the cell-wall of flor yeasts. These findings reveal a novel biological activity of L-histidine in controlling the multicellular behavior of yeasts [corrected].

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Loss of adhesion in presence of L-histidine and other dipeptides.Adhesion is expressed as OD570 and was measured using crystal violet dye after 48 h incubation of 5×106 cell/mL of the S. cerevisiae strains in flor medium without (Ctrl) and with 10 mM L-histidine or the L-histidine–containing peptides.
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pone-0112141-g004: Loss of adhesion in presence of L-histidine and other dipeptides.Adhesion is expressed as OD570 and was measured using crystal violet dye after 48 h incubation of 5×106 cell/mL of the S. cerevisiae strains in flor medium without (Ctrl) and with 10 mM L-histidine or the L-histidine–containing peptides.

Mentions: Adhesion to polystyrene was evaluated after 48 h incubation in flor medium without or with 10 mM L-histidine or 10 mM dipeptides. S288c and 3238-32Δflo11 showed very low adhesion after 48 h, as expected for strains lacking Flo11p. However, strains A9, M23, V80, and 3238-32, which were highly adhesive in the absence of L-histidine, showed drastic reductions in their adhesion to polystyrene in the presence of L-histidine and the three dipeptides HV, HM and HS (Fig. 4).


L-histidine inhibits biofilm formation and FLO11-associated phenotypes in Saccharomyces cerevisiae flor yeasts.

Bou Zeidan M, Zara G, Viti C, Decorosi F, Mannazzu I, Budroni M, Giovannetti L, Zara S - PLoS ONE (2014)

Loss of adhesion in presence of L-histidine and other dipeptides.Adhesion is expressed as OD570 and was measured using crystal violet dye after 48 h incubation of 5×106 cell/mL of the S. cerevisiae strains in flor medium without (Ctrl) and with 10 mM L-histidine or the L-histidine–containing peptides.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219837&req=5

pone-0112141-g004: Loss of adhesion in presence of L-histidine and other dipeptides.Adhesion is expressed as OD570 and was measured using crystal violet dye after 48 h incubation of 5×106 cell/mL of the S. cerevisiae strains in flor medium without (Ctrl) and with 10 mM L-histidine or the L-histidine–containing peptides.
Mentions: Adhesion to polystyrene was evaluated after 48 h incubation in flor medium without or with 10 mM L-histidine or 10 mM dipeptides. S288c and 3238-32Δflo11 showed very low adhesion after 48 h, as expected for strains lacking Flo11p. However, strains A9, M23, V80, and 3238-32, which were highly adhesive in the absence of L-histidine, showed drastic reductions in their adhesion to polystyrene in the presence of L-histidine and the three dipeptides HV, HM and HS (Fig. 4).

Bottom Line: L-histidine completely inhibited growth and its effect on viability was inversely related to Flo11p expression.Accordingly, L-histidine did not affect the viability of the Δflo11 and S288c strains.Also, L-histidine dramatically decreased air-liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of the Flo11p gene.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Agraria, University of Sassari, Sassari, Italy.

ABSTRACT
Flor yeasts of Saccharomyces cerevisiae have an innate diversity of Flo11p which codes for a highly hydrophobic and anionic cell-wall glycoprotein with a fundamental role in biofilm formation. In this study, 380 nitrogen compounds were administered to three S. cerevisiae flor strains handling Flo11p alleles with different expression levels. S. cerevisiae strain S288c was used as the reference strain as it cannot produce Flo11p. The flor strains generally metabolized amino acids and dipeptides as the sole nitrogen source, although with some exceptions regarding L-histidine and histidine containing dipeptides. L-histidine completely inhibited growth and its effect on viability was inversely related to Flo11p expression. Accordingly, L-histidine did not affect the viability of the Δflo11 and S288c strains. Also, L-histidine dramatically decreased air-liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of the Flo11p gene. Moreover, L-histidine modified the chitin and glycans content on the cell-wall of flor yeasts. These findings reveal a novel biological activity of L-histidine in controlling the multicellular behavior of yeasts [corrected].

Show MeSH
Related in: MedlinePlus