Limits...
L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets.

Lin M, Zhang B, Yu C, Li J, Zhang L, Sun H, Gao F, Zhou G - PLoS ONE (2014)

Bottom Line: However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine.The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets.These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu, China.

ABSTRACT
L-Glutamate is a major oxidative fuel for the small intestine. However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. A total of 120 weaning piglets aged 35 ± 1 days with an average body weight at 8.91 ± 0.45 kg were randomly allocated to two treatments with six replicates of ten piglets each, fed with diets containing 1.21% alanine, or 2% L-glutamate. L-Glutamate supplementation increased the activity of glutamate oxaloacetate transaminase (GOT) in the jejunal mucosa. Also, the mRNA expression level of jejunal mucosa glutamine synthetase (GS) was increased by L-glutamate supplementation. The height of villi in duodenal and jejunal segments, and the relative mRNA expression of occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa were increased by dietary L-glutamate supplementation. L-Glutamate supplementation increased plasma concentrations of glutamate, arginine, histidine, isoleucine, leucine, methionine, phenylalanine and threonine. L-Glutamate supplementation also increased the relative mRNA expression of the jejunal mucosa Ca(2+)-sensing receptor (CaR), metabotropic glutamate receptor 1 (mGluR1) and metabotropic glutamate receptor 4 (mGluR4), and neutral amino acid transporter B(0)-like (SLC1A5) in the jejunal mucosa. These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.

Show MeSH
Relative mRNA expression of jejunal moucosa glutamine synthetase (GS) and glutamate dehydrogenase 1(GLUD1).Ala, diet supplemented with 12.1 g alanine/kg, Glu, diet supplemented with 20 g glutamate/kg. mRNA expression levels were normalized using β-actin as an internal control. Values are expressed as mean±SEM. a, bMean values within different letters were significantly different (P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4219819&req=5

pone-0111950-g001: Relative mRNA expression of jejunal moucosa glutamine synthetase (GS) and glutamate dehydrogenase 1(GLUD1).Ala, diet supplemented with 12.1 g alanine/kg, Glu, diet supplemented with 20 g glutamate/kg. mRNA expression levels were normalized using β-actin as an internal control. Values are expressed as mean±SEM. a, bMean values within different letters were significantly different (P<0.05).

Mentions: The abundances of GS and GLUD1 mRNAs in the jejunal mucosa of weaning piglets are shown in Fig. 1. Dietary L-glutamate supplementation increased the mRNA expression of jejunal mucosa GS (P<0.05), but did not affect the mRNA expression of jejunal mucosa GLUD1.


L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets.

Lin M, Zhang B, Yu C, Li J, Zhang L, Sun H, Gao F, Zhou G - PLoS ONE (2014)

Relative mRNA expression of jejunal moucosa glutamine synthetase (GS) and glutamate dehydrogenase 1(GLUD1).Ala, diet supplemented with 12.1 g alanine/kg, Glu, diet supplemented with 20 g glutamate/kg. mRNA expression levels were normalized using β-actin as an internal control. Values are expressed as mean±SEM. a, bMean values within different letters were significantly different (P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219819&req=5

pone-0111950-g001: Relative mRNA expression of jejunal moucosa glutamine synthetase (GS) and glutamate dehydrogenase 1(GLUD1).Ala, diet supplemented with 12.1 g alanine/kg, Glu, diet supplemented with 20 g glutamate/kg. mRNA expression levels were normalized using β-actin as an internal control. Values are expressed as mean±SEM. a, bMean values within different letters were significantly different (P<0.05).
Mentions: The abundances of GS and GLUD1 mRNAs in the jejunal mucosa of weaning piglets are shown in Fig. 1. Dietary L-glutamate supplementation increased the mRNA expression of jejunal mucosa GS (P<0.05), but did not affect the mRNA expression of jejunal mucosa GLUD1.

Bottom Line: However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine.The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets.These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu, China.

ABSTRACT
L-Glutamate is a major oxidative fuel for the small intestine. However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. A total of 120 weaning piglets aged 35 ± 1 days with an average body weight at 8.91 ± 0.45 kg were randomly allocated to two treatments with six replicates of ten piglets each, fed with diets containing 1.21% alanine, or 2% L-glutamate. L-Glutamate supplementation increased the activity of glutamate oxaloacetate transaminase (GOT) in the jejunal mucosa. Also, the mRNA expression level of jejunal mucosa glutamine synthetase (GS) was increased by L-glutamate supplementation. The height of villi in duodenal and jejunal segments, and the relative mRNA expression of occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa were increased by dietary L-glutamate supplementation. L-Glutamate supplementation increased plasma concentrations of glutamate, arginine, histidine, isoleucine, leucine, methionine, phenylalanine and threonine. L-Glutamate supplementation also increased the relative mRNA expression of the jejunal mucosa Ca(2+)-sensing receptor (CaR), metabotropic glutamate receptor 1 (mGluR1) and metabotropic glutamate receptor 4 (mGluR4), and neutral amino acid transporter B(0)-like (SLC1A5) in the jejunal mucosa. These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.

Show MeSH