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A metabolic profiling strategy for the dissection of plant defense against fungal pathogens.

Aliferis KA, Faubert D, Jabaji S - PLoS ONE (2014)

Bottom Line: Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome.Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion.Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Science, Macdonald Campus of McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada.

ABSTRACT
Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome. The study of metabolic networks revealed that infection results in the mobilization of carbohydrates, disturbance of the amino acid pool, and activation of isoflavonoid, α-linolenate, and phenylpropanoid biosynthetic pathways of the plant. Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion. Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries.

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Proposed graphical model for the role of Rhizoctonia solani in activation of soybean defense mechanisms.[CM; cell membrane, CW; cell wall, G; Golgi body, M; mitochondrion, N; nucleus, P; plasmalemma, R; ribosomes, RER; rough endoplasmic reticulum, SER; soft endoplasmic reticulum, T; transporters, V; vacuole].
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pone-0111930-g006: Proposed graphical model for the role of Rhizoctonia solani in activation of soybean defense mechanisms.[CM; cell membrane, CW; cell wall, G; Golgi body, M; mitochondrion, N; nucleus, P; plasmalemma, R; ribosomes, RER; rough endoplasmic reticulum, SER; soft endoplasmic reticulum, T; transporters, V; vacuole].

Mentions: Analysis focused not only on temporal metabolite changes but also on global metabolic network regulation in response to fungal infection (Fig. 6). The most up- and down-regulated soybean pathways are summarized in Table 1.


A metabolic profiling strategy for the dissection of plant defense against fungal pathogens.

Aliferis KA, Faubert D, Jabaji S - PLoS ONE (2014)

Proposed graphical model for the role of Rhizoctonia solani in activation of soybean defense mechanisms.[CM; cell membrane, CW; cell wall, G; Golgi body, M; mitochondrion, N; nucleus, P; plasmalemma, R; ribosomes, RER; rough endoplasmic reticulum, SER; soft endoplasmic reticulum, T; transporters, V; vacuole].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219818&req=5

pone-0111930-g006: Proposed graphical model for the role of Rhizoctonia solani in activation of soybean defense mechanisms.[CM; cell membrane, CW; cell wall, G; Golgi body, M; mitochondrion, N; nucleus, P; plasmalemma, R; ribosomes, RER; rough endoplasmic reticulum, SER; soft endoplasmic reticulum, T; transporters, V; vacuole].
Mentions: Analysis focused not only on temporal metabolite changes but also on global metabolic network regulation in response to fungal infection (Fig. 6). The most up- and down-regulated soybean pathways are summarized in Table 1.

Bottom Line: Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome.Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion.Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Science, Macdonald Campus of McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada.

ABSTRACT
Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome. The study of metabolic networks revealed that infection results in the mobilization of carbohydrates, disturbance of the amino acid pool, and activation of isoflavonoid, α-linolenate, and phenylpropanoid biosynthetic pathways of the plant. Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion. Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries.

Show MeSH
Related in: MedlinePlus