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Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

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Evaluation of femoral morphology changes between control and CIA groups.(A) Cartilage volume. (B) Cartilage surface area. (C) Average cartilage thickness. (D) Bone volume to tissue volume ratio (BV/TV). (E) Bone surface to bone volume ratio (BS/BV). (F) Trabecular bone thickness (Tb.Th). (G) Trabecular bone number (Tb.N). (H) Trabecular bone space (Tb.Sp). Data are presented as the mean±SD. *: P<0.05 for differences between the control group and the CIA group; #: P<0.05 for differences within the CIA group.
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pone-0111939-g009: Evaluation of femoral morphology changes between control and CIA groups.(A) Cartilage volume. (B) Cartilage surface area. (C) Average cartilage thickness. (D) Bone volume to tissue volume ratio (BV/TV). (E) Bone surface to bone volume ratio (BS/BV). (F) Trabecular bone thickness (Tb.Th). (G) Trabecular bone number (Tb.N). (H) Trabecular bone space (Tb.Sp). Data are presented as the mean±SD. *: P<0.05 for differences between the control group and the CIA group; #: P<0.05 for differences within the CIA group.

Mentions: To objectively analyse the structural changes in the articular cartilage and subchondral bone in a quantitative manner, the femoral 3D morphologic parameters of two treatment groups (the control group and CIA group) were measured (Figure 9). At 4 weeks after the primary injection, the femoral cartilage volume, surface area and average thickness in the CIA group were 11%, 15% and 12% lower, respectively, than in the control group (P<0.05). In addition, BV/TV, Tb.Th and Tb.N in the CIA group were 6%, 11% and 14% lower, respectively, than in the control group (P<0.05), whereas BS/BV and Th.Sp were 10% and 16% higher, respectively (P<0.05). At 8 weeks after the primary injection, the cartilage volume, surface area and average thickness in the CIA group were decreased by 32%, 28% and 26%, respectively, compared to the control group (P<0.05). Moreover, BV/TV, Tb.Th and Tb.N in the CIA groups were decreased by 28%, 29% and 34%, respectively, corresponding increases of 18% and 43% were measured for BS/BV and Tb.Sp, respectively, compared to the control group (P<0.05).


Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

Evaluation of femoral morphology changes between control and CIA groups.(A) Cartilage volume. (B) Cartilage surface area. (C) Average cartilage thickness. (D) Bone volume to tissue volume ratio (BV/TV). (E) Bone surface to bone volume ratio (BS/BV). (F) Trabecular bone thickness (Tb.Th). (G) Trabecular bone number (Tb.N). (H) Trabecular bone space (Tb.Sp). Data are presented as the mean±SD. *: P<0.05 for differences between the control group and the CIA group; #: P<0.05 for differences within the CIA group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219817&req=5

pone-0111939-g009: Evaluation of femoral morphology changes between control and CIA groups.(A) Cartilage volume. (B) Cartilage surface area. (C) Average cartilage thickness. (D) Bone volume to tissue volume ratio (BV/TV). (E) Bone surface to bone volume ratio (BS/BV). (F) Trabecular bone thickness (Tb.Th). (G) Trabecular bone number (Tb.N). (H) Trabecular bone space (Tb.Sp). Data are presented as the mean±SD. *: P<0.05 for differences between the control group and the CIA group; #: P<0.05 for differences within the CIA group.
Mentions: To objectively analyse the structural changes in the articular cartilage and subchondral bone in a quantitative manner, the femoral 3D morphologic parameters of two treatment groups (the control group and CIA group) were measured (Figure 9). At 4 weeks after the primary injection, the femoral cartilage volume, surface area and average thickness in the CIA group were 11%, 15% and 12% lower, respectively, than in the control group (P<0.05). In addition, BV/TV, Tb.Th and Tb.N in the CIA group were 6%, 11% and 14% lower, respectively, than in the control group (P<0.05), whereas BS/BV and Th.Sp were 10% and 16% higher, respectively (P<0.05). At 8 weeks after the primary injection, the cartilage volume, surface area and average thickness in the CIA group were decreased by 32%, 28% and 26%, respectively, compared to the control group (P<0.05). Moreover, BV/TV, Tb.Th and Tb.N in the CIA groups were decreased by 28%, 29% and 34%, respectively, corresponding increases of 18% and 43% were measured for BS/BV and Tb.Sp, respectively, compared to the control group (P<0.05).

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

Show MeSH
Related in: MedlinePlus