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Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

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Related in: MedlinePlus

The 3D morphologic images of Trabecular bone.(A) Trabecular bone from a mouse in control group at 0, 4 and 8 weeks after the primary injection. (B) Trabecular bone from a mouse in CIA group at 0, 4 and 8 weeks after the primary injection. The red arrowheads indicate fragments of trabecular bone.
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pone-0111939-g008: The 3D morphologic images of Trabecular bone.(A) Trabecular bone from a mouse in control group at 0, 4 and 8 weeks after the primary injection. (B) Trabecular bone from a mouse in CIA group at 0, 4 and 8 weeks after the primary injection. The red arrowheads indicate fragments of trabecular bone.

Mentions: The surface rendering method was used to visualise the femur, which provided an intuitive means of identifying 3D architectural anomalies in the femoral cartilage, subchondral bone surface and trabecular bone. Representative 3D images are respectively shown in Figure 6, 7 and 8. At 0 weeks after the primary injection, the structure of femur was basically intact, and no cartilage erosion, subchondral bone lamella denudation or trabecular bone deformities were apparent. As time progressed, we observed that the outer edge of the femoral cartilage, where synovium is densely distributed, was eroded relatively earlier. At later time points, the cartilage damage gradually expanded from the outside to the inside and had an aggravating tendency with the development of the disease. Meanwhile, the subchondral bone was also invaded. Part of the subchondral bone surface was destroyed at 4 weeks, particularly in regions without cartilage coverage, and it had further deteriorated at 8 weeks. At 4 weeks, obvious architectural destruction of the trabecular bone was observed in the form of decreased trabecular bone number and increased trabecular bone separation at 4 weeks. At 8 weeks, there was a progressive increase in the number of bone fragments compared to 4 weeks.


Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

The 3D morphologic images of Trabecular bone.(A) Trabecular bone from a mouse in control group at 0, 4 and 8 weeks after the primary injection. (B) Trabecular bone from a mouse in CIA group at 0, 4 and 8 weeks after the primary injection. The red arrowheads indicate fragments of trabecular bone.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219817&req=5

pone-0111939-g008: The 3D morphologic images of Trabecular bone.(A) Trabecular bone from a mouse in control group at 0, 4 and 8 weeks after the primary injection. (B) Trabecular bone from a mouse in CIA group at 0, 4 and 8 weeks after the primary injection. The red arrowheads indicate fragments of trabecular bone.
Mentions: The surface rendering method was used to visualise the femur, which provided an intuitive means of identifying 3D architectural anomalies in the femoral cartilage, subchondral bone surface and trabecular bone. Representative 3D images are respectively shown in Figure 6, 7 and 8. At 0 weeks after the primary injection, the structure of femur was basically intact, and no cartilage erosion, subchondral bone lamella denudation or trabecular bone deformities were apparent. As time progressed, we observed that the outer edge of the femoral cartilage, where synovium is densely distributed, was eroded relatively earlier. At later time points, the cartilage damage gradually expanded from the outside to the inside and had an aggravating tendency with the development of the disease. Meanwhile, the subchondral bone was also invaded. Part of the subchondral bone surface was destroyed at 4 weeks, particularly in regions without cartilage coverage, and it had further deteriorated at 8 weeks. At 4 weeks, obvious architectural destruction of the trabecular bone was observed in the form of decreased trabecular bone number and increased trabecular bone separation at 4 weeks. At 8 weeks, there was a progressive increase in the number of bone fragments compared to 4 weeks.

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

Show MeSH
Related in: MedlinePlus