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Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

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Related in: MedlinePlus

Histological images of femur sections stained with Safranin-O.(A) Femur sections from control group at 0, 4 and 8 weeks after the primary injection. (B) Femur sections from CIA group at 0, 4 and 8 weeks after the primary injection. (C) Average thickness of femoral cartilage assessed by histology. Data are presented as the the mean±SD. 2D CT image before image preprocessing. 2D CT image before image preprocessing. #: P<0.05 for differences between the control group and the CIA group. Scale bar: 20 µm.
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pone-0111939-g004: Histological images of femur sections stained with Safranin-O.(A) Femur sections from control group at 0, 4 and 8 weeks after the primary injection. (B) Femur sections from CIA group at 0, 4 and 8 weeks after the primary injection. (C) Average thickness of femoral cartilage assessed by histology. Data are presented as the the mean±SD. 2D CT image before image preprocessing. 2D CT image before image preprocessing. #: P<0.05 for differences between the control group and the CIA group. Scale bar: 20 µm.

Mentions: Representative images of femur sections stained with Safranin-O and the from control and CIA group are shown in Figure 4. At 0 weeks after primary injection, the contour line of the femoral cartilage layer was relatively smooth and the subchondral bone was basically intact. In contrast, at 4 and 8 weeks after primary injection, the thickness of the femoral cartilage was decreased significantly and the contour line was not smooth. Furthermore, part of the subchondral bone surface was obviously eroded. By statistic analysis, the average thickness of femoral cartilage form CIA group at 4 and 8 weeks were respectively low 21% and 34% compared to control group (P<0.05).


Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

Histological images of femur sections stained with Safranin-O.(A) Femur sections from control group at 0, 4 and 8 weeks after the primary injection. (B) Femur sections from CIA group at 0, 4 and 8 weeks after the primary injection. (C) Average thickness of femoral cartilage assessed by histology. Data are presented as the the mean±SD. 2D CT image before image preprocessing. 2D CT image before image preprocessing. #: P<0.05 for differences between the control group and the CIA group. Scale bar: 20 µm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219817&req=5

pone-0111939-g004: Histological images of femur sections stained with Safranin-O.(A) Femur sections from control group at 0, 4 and 8 weeks after the primary injection. (B) Femur sections from CIA group at 0, 4 and 8 weeks after the primary injection. (C) Average thickness of femoral cartilage assessed by histology. Data are presented as the the mean±SD. 2D CT image before image preprocessing. 2D CT image before image preprocessing. #: P<0.05 for differences between the control group and the CIA group. Scale bar: 20 µm.
Mentions: Representative images of femur sections stained with Safranin-O and the from control and CIA group are shown in Figure 4. At 0 weeks after primary injection, the contour line of the femoral cartilage layer was relatively smooth and the subchondral bone was basically intact. In contrast, at 4 and 8 weeks after primary injection, the thickness of the femoral cartilage was decreased significantly and the contour line was not smooth. Furthermore, part of the subchondral bone surface was obviously eroded. By statistic analysis, the average thickness of femoral cartilage form CIA group at 4 and 8 weeks were respectively low 21% and 34% compared to control group (P<0.05).

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

Show MeSH
Related in: MedlinePlus