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Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

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Related in: MedlinePlus

Before and after CT image via image preprocessing.(A) 2D CT image without image preprocessing. (B) 2D CT image after image preprocessing.
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pone-0111939-g002: Before and after CT image via image preprocessing.(A) 2D CT image without image preprocessing. (B) 2D CT image after image preprocessing.

Mentions: After the above steps, the conventional filtered back projection (FBP) algorithm with a Hamming filter was used to reconstruct CT slices from the 720 projections. The femora were placed in a vertical position during scanning. Therefore, a series of transverse cross-sectional slices was produced. Before and after CT image of removing ring artifacts are shown in Figure 2. We found that the ring artifacts were suppressed effectively, and the quality of image had improved significantly.


Quantitative assessment of murine articular cartilage and bone using X-ray phase-contrast imaging.

Li J, Yuan H, Wu M, Dong L, Zhang L, Shi H, Luo S - PLoS ONE (2014)

Before and after CT image via image preprocessing.(A) 2D CT image without image preprocessing. (B) 2D CT image after image preprocessing.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219817&req=5

pone-0111939-g002: Before and after CT image via image preprocessing.(A) 2D CT image without image preprocessing. (B) 2D CT image after image preprocessing.
Mentions: After the above steps, the conventional filtered back projection (FBP) algorithm with a Hamming filter was used to reconstruct CT slices from the 720 projections. The femora were placed in a vertical position during scanning. Therefore, a series of transverse cross-sectional slices was produced. Before and after CT image of removing ring artifacts are shown in Figure 2. We found that the ring artifacts were suppressed effectively, and the quality of image had improved significantly.

Bottom Line: Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group.Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group.This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.

ABSTRACT
Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

Show MeSH
Related in: MedlinePlus