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Itch is required for lateral line development in zebrafish.

Angers A, Drapeau P - PLoS ONE (2014)

Bottom Line: The zebrafish posterior lateral line is formed during early development by the deposition of neuromasts from a migrating primordium.The molecular mechanisms regulating the regional organization and migration of the primordium involve interactions between Fgf and Wnt/β-catenin signaling and the establishment of specific cxcr4b and cxcr7b cytokine receptor expression domains.Itch knockdown results in a failure to down-regulate Wnt signaling and overexpression of cxcr4b in the primordium, slowing migration of the posterior lateral line primordium and resulting in abnormal development of the lateral line.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Montreal, Montreal, Quebec, Canada.

ABSTRACT
The zebrafish posterior lateral line is formed during early development by the deposition of neuromasts from a migrating primordium. The molecular mechanisms regulating the regional organization and migration of the primordium involve interactions between Fgf and Wnt/β-catenin signaling and the establishment of specific cxcr4b and cxcr7b cytokine receptor expression domains. Itch has been identified as a regulator in several different signaling pathways, including Wnt and Cxcr4 signaling. We identified two homologous itch genes in zebrafish, itcha and itchb, with generalized expression patterns. By reducing itchb expression in particular upon morpholino knockdown, we demonstrated the importance of Itch in regulating lateral line development by perturbing the patterns of cxcr4b and cxcr7b expression. Itch knockdown results in a failure to down-regulate Wnt signaling and overexpression of cxcr4b in the primordium, slowing migration of the posterior lateral line primordium and resulting in abnormal development of the lateral line.

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Phylogenetic tree of human and zebrafish members of the CWH family of E3s.Two zebrafish sequences cluster with the human ITCH gene. The tree was constructed with the neighbor-joining method supported by 1000 bootstraps. Sequences used in the phylogenetic analysis are given in Table S1.
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pone-0111799-g001: Phylogenetic tree of human and zebrafish members of the CWH family of E3s.Two zebrafish sequences cluster with the human ITCH gene. The tree was constructed with the neighbor-joining method supported by 1000 bootstraps. Sequences used in the phylogenetic analysis are given in Table S1.

Mentions: We aligned these cds sequences together with the human sequences for all nine members of the CWH sub-family (Table S1), and constructed a phylogenetic tree using the Phylip package SeqDist algorithm (Fig. 1) [22]. All zebrafish sequences clustered with their human counterparts, giving weight to the hypothesis that they represent orthologues. Two genes grouped with human ITCH. We designate these sequences itcha and itchb, corresponding respectively to NCBI gene IDs 100331274 on chromosome 6 and 100330031 on chromosome 23. Interestingly, the itchb sequence is absent from the ZFIN database but was however confirmed in the latest zebrafish genome sequencing project [23]. Except for itch and nedl2, only one copy of each of the other CWHs was found in the zebrafish genome.


Itch is required for lateral line development in zebrafish.

Angers A, Drapeau P - PLoS ONE (2014)

Phylogenetic tree of human and zebrafish members of the CWH family of E3s.Two zebrafish sequences cluster with the human ITCH gene. The tree was constructed with the neighbor-joining method supported by 1000 bootstraps. Sequences used in the phylogenetic analysis are given in Table S1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219781&req=5

pone-0111799-g001: Phylogenetic tree of human and zebrafish members of the CWH family of E3s.Two zebrafish sequences cluster with the human ITCH gene. The tree was constructed with the neighbor-joining method supported by 1000 bootstraps. Sequences used in the phylogenetic analysis are given in Table S1.
Mentions: We aligned these cds sequences together with the human sequences for all nine members of the CWH sub-family (Table S1), and constructed a phylogenetic tree using the Phylip package SeqDist algorithm (Fig. 1) [22]. All zebrafish sequences clustered with their human counterparts, giving weight to the hypothesis that they represent orthologues. Two genes grouped with human ITCH. We designate these sequences itcha and itchb, corresponding respectively to NCBI gene IDs 100331274 on chromosome 6 and 100330031 on chromosome 23. Interestingly, the itchb sequence is absent from the ZFIN database but was however confirmed in the latest zebrafish genome sequencing project [23]. Except for itch and nedl2, only one copy of each of the other CWHs was found in the zebrafish genome.

Bottom Line: The zebrafish posterior lateral line is formed during early development by the deposition of neuromasts from a migrating primordium.The molecular mechanisms regulating the regional organization and migration of the primordium involve interactions between Fgf and Wnt/β-catenin signaling and the establishment of specific cxcr4b and cxcr7b cytokine receptor expression domains.Itch knockdown results in a failure to down-regulate Wnt signaling and overexpression of cxcr4b in the primordium, slowing migration of the posterior lateral line primordium and resulting in abnormal development of the lateral line.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Montreal, Montreal, Quebec, Canada.

ABSTRACT
The zebrafish posterior lateral line is formed during early development by the deposition of neuromasts from a migrating primordium. The molecular mechanisms regulating the regional organization and migration of the primordium involve interactions between Fgf and Wnt/β-catenin signaling and the establishment of specific cxcr4b and cxcr7b cytokine receptor expression domains. Itch has been identified as a regulator in several different signaling pathways, including Wnt and Cxcr4 signaling. We identified two homologous itch genes in zebrafish, itcha and itchb, with generalized expression patterns. By reducing itchb expression in particular upon morpholino knockdown, we demonstrated the importance of Itch in regulating lateral line development by perturbing the patterns of cxcr4b and cxcr7b expression. Itch knockdown results in a failure to down-regulate Wnt signaling and overexpression of cxcr4b in the primordium, slowing migration of the posterior lateral line primordium and resulting in abnormal development of the lateral line.

Show MeSH
Related in: MedlinePlus