Limits...
Fungicide effects on fungal community composition in the wheat phyllosphere.

Karlsson I, Friberg H, Steinberg C, Persson P - PLoS ONE (2014)

Bottom Line: This study examined fungicide effects on fungal communities on winter wheat leaves in two areas of Sweden.It was found that commonly used fungicides had moderate but significant effect on fungal community composition in the wheat phyllosphere.The relative abundance of several saprotrophs was altered by fungicide use, while the effect on common wheat pathogens was mixed.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Crop Production Ecology, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.

ABSTRACT
The fungicides used to control diseases in cereal production can have adverse effects on non-target fungi, with possible consequences for plant health and productivity. This study examined fungicide effects on fungal communities on winter wheat leaves in two areas of Sweden. High-throughput 454 sequencing of the fungal ITS2 region yielded 235 operational taxonomic units (OTUs) at the species level from the 18 fields studied. It was found that commonly used fungicides had moderate but significant effect on fungal community composition in the wheat phyllosphere. The relative abundance of several saprotrophs was altered by fungicide use, while the effect on common wheat pathogens was mixed. The fungal community on wheat leaves consisted mainly of basidiomycete yeasts, saprotrophic ascomycetes and plant pathogens. A core set of six fungal OTUs representing saprotrophic species was identified. These were present across all fields, although overall the difference in OTU richness was large between the two areas studied.

Show MeSH
Rarefaction and species accumulation curves.a) Rarefaction curves presenting the relationship between sequencing depth and species richness in operational taxonomic units (OTUs). Error bars indicate 95% confidence intervals. b) Sample-based species accumulation curves. When more than two samples were taken per field, one fungicide-treated and one untreated sample was randomly chosen from each field for inclusion in the analysis. Error bars indicate 95% confidence intervals, only shown for Northern and Southern area respectively.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4219778&req=5

pone-0111786-g002: Rarefaction and species accumulation curves.a) Rarefaction curves presenting the relationship between sequencing depth and species richness in operational taxonomic units (OTUs). Error bars indicate 95% confidence intervals. b) Sample-based species accumulation curves. When more than two samples were taken per field, one fungicide-treated and one untreated sample was randomly chosen from each field for inclusion in the analysis. Error bars indicate 95% confidence intervals, only shown for Northern and Southern area respectively.

Mentions: The fungal community composition in the wheat phyllosphere was characterised using 454 high-throughput sequencing. We found 235 fungal OTUs in the pool of 420 wheat leaves when clustering at 1.5% dissimilarity level. The rarefaction curves approached saturation (Fig. 2a) for all conditions, while the species accumulation curves did not (Fig. 2b).


Fungicide effects on fungal community composition in the wheat phyllosphere.

Karlsson I, Friberg H, Steinberg C, Persson P - PLoS ONE (2014)

Rarefaction and species accumulation curves.a) Rarefaction curves presenting the relationship between sequencing depth and species richness in operational taxonomic units (OTUs). Error bars indicate 95% confidence intervals. b) Sample-based species accumulation curves. When more than two samples were taken per field, one fungicide-treated and one untreated sample was randomly chosen from each field for inclusion in the analysis. Error bars indicate 95% confidence intervals, only shown for Northern and Southern area respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219778&req=5

pone-0111786-g002: Rarefaction and species accumulation curves.a) Rarefaction curves presenting the relationship between sequencing depth and species richness in operational taxonomic units (OTUs). Error bars indicate 95% confidence intervals. b) Sample-based species accumulation curves. When more than two samples were taken per field, one fungicide-treated and one untreated sample was randomly chosen from each field for inclusion in the analysis. Error bars indicate 95% confidence intervals, only shown for Northern and Southern area respectively.
Mentions: The fungal community composition in the wheat phyllosphere was characterised using 454 high-throughput sequencing. We found 235 fungal OTUs in the pool of 420 wheat leaves when clustering at 1.5% dissimilarity level. The rarefaction curves approached saturation (Fig. 2a) for all conditions, while the species accumulation curves did not (Fig. 2b).

Bottom Line: This study examined fungicide effects on fungal communities on winter wheat leaves in two areas of Sweden.It was found that commonly used fungicides had moderate but significant effect on fungal community composition in the wheat phyllosphere.The relative abundance of several saprotrophs was altered by fungicide use, while the effect on common wheat pathogens was mixed.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Crop Production Ecology, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.

ABSTRACT
The fungicides used to control diseases in cereal production can have adverse effects on non-target fungi, with possible consequences for plant health and productivity. This study examined fungicide effects on fungal communities on winter wheat leaves in two areas of Sweden. High-throughput 454 sequencing of the fungal ITS2 region yielded 235 operational taxonomic units (OTUs) at the species level from the 18 fields studied. It was found that commonly used fungicides had moderate but significant effect on fungal community composition in the wheat phyllosphere. The relative abundance of several saprotrophs was altered by fungicide use, while the effect on common wheat pathogens was mixed. The fungal community on wheat leaves consisted mainly of basidiomycete yeasts, saprotrophic ascomycetes and plant pathogens. A core set of six fungal OTUs representing saprotrophic species was identified. These were present across all fields, although overall the difference in OTU richness was large between the two areas studied.

Show MeSH