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Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.

Niedojadło J, Kubicka E, Kalich B, Smoliński DJ - PLoS ONE (2014)

Bottom Line: In both types of nuclei, the amount of poly(A) RNA was much greater in the nucleus than in the cytoplasm.In addition, it was demonstrated that accumulation of poly(A) RNA occurs in the nuclei and CBs of hypoxia-treated cells.Our findings indicated that CBs may be involved in the later stages of poly(A) RNA metabolism, playing a role storage or retention.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT
The localisation of poly(A) RNA in plant cells containing either reticular (Allium cepa) or chromocentric (Lupinus luteus, Arabidopsis thaliana) nuclei was studied through in situ hybridisation. In both types of nuclei, the amount of poly(A) RNA was much greater in the nucleus than in the cytoplasm. In the nuclei, poly(A) RNA was present in structures resembling nuclear bodies. The molecular composition as well as the characteristic ultrastructure of the bodies containing poly(A) RNA demonstrated that they were Cajal bodies. We showed that some poly(A) RNAs in Cajal bodies code for proteins. However, examination of the localisation of active RNA polymerase II and in situ run-on transcription assays both demonstrated that CBs are not sites of transcription and that BrU-containing RNA accumulates in these structures long after synthesis. In addition, it was demonstrated that accumulation of poly(A) RNA occurs in the nuclei and CBs of hypoxia-treated cells. Our findings indicated that CBs may be involved in the later stages of poly(A) RNA metabolism, playing a role storage or retention.

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Double labelling of poly(A) RNA and the PANA antigen in the chromocentric (Lupinus) (A–C) and reticular (Allium) (D–F) nuclei of root cells.In the nucleoplasm of both species, poly(A) RNA is present in nuclear structures (arrows) and does not colocalise with speckles. Representative examples of Pearson correlation coefficients for weak and non-colocalisation of poly(A) mRNA with the PANA antigen in Lupinus luteus and Allium cepa cells (G). A scale bar representing 2 µm is shown. The percentages of weak and non-colocalisation of poly(A) RNA-rich bodies with the PANA antigen are indicated by the Pearson correlation coefficient (H). Error bars represent standard error. Double labelling of poly(A) RNA and U2 snRNA in Lupinus (I–K) and Allium (L–N) cells. Accumulation of poly(A) RNA in nuclear bodies rich in U2 snRNA (arrows). Bar, 5 µm. N-nucleus, Nu- nucleolus
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pone-0111780-g002: Double labelling of poly(A) RNA and the PANA antigen in the chromocentric (Lupinus) (A–C) and reticular (Allium) (D–F) nuclei of root cells.In the nucleoplasm of both species, poly(A) RNA is present in nuclear structures (arrows) and does not colocalise with speckles. Representative examples of Pearson correlation coefficients for weak and non-colocalisation of poly(A) mRNA with the PANA antigen in Lupinus luteus and Allium cepa cells (G). A scale bar representing 2 µm is shown. The percentages of weak and non-colocalisation of poly(A) RNA-rich bodies with the PANA antigen are indicated by the Pearson correlation coefficient (H). Error bars represent standard error. Double labelling of poly(A) RNA and U2 snRNA in Lupinus (I–K) and Allium (L–N) cells. Accumulation of poly(A) RNA in nuclear bodies rich in U2 snRNA (arrows). Bar, 5 µm. N-nucleus, Nu- nucleolus

Mentions: We have shown that in the cells of plant roots, a considerably larger amount of poly(A) RNA is present in the nucleus than in the cytoplasm (up to more than 80% in lupine) (Figure 1). In the nucleoplasm, the signal forms clusters of different sizes, in both chromocentric (Lupinus luteus) (Figure 2A, C) and reticular (Allium cepa) nuclei (Figure 2D, F). Some of these clusters resemble nuclear bodies in terms of their shape and distribution. In lupine, the nuclear bodies enriched in poly(A) mRNA were more numerous and smaller in meristematic cells, whereas there were isolated but larger examples observed in differentiated cells of the roots (Figure S1A, B). These round clusters were observed in 87% of lupine root cells. Quantitative measurements have shown that in the meristematic cells of lupine plants, approximately 3.5% of the nuclear pool of the poly(A) RNA occurs in structures resembling nuclear bodies (Figure 1).


Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.

Niedojadło J, Kubicka E, Kalich B, Smoliński DJ - PLoS ONE (2014)

Double labelling of poly(A) RNA and the PANA antigen in the chromocentric (Lupinus) (A–C) and reticular (Allium) (D–F) nuclei of root cells.In the nucleoplasm of both species, poly(A) RNA is present in nuclear structures (arrows) and does not colocalise with speckles. Representative examples of Pearson correlation coefficients for weak and non-colocalisation of poly(A) mRNA with the PANA antigen in Lupinus luteus and Allium cepa cells (G). A scale bar representing 2 µm is shown. The percentages of weak and non-colocalisation of poly(A) RNA-rich bodies with the PANA antigen are indicated by the Pearson correlation coefficient (H). Error bars represent standard error. Double labelling of poly(A) RNA and U2 snRNA in Lupinus (I–K) and Allium (L–N) cells. Accumulation of poly(A) RNA in nuclear bodies rich in U2 snRNA (arrows). Bar, 5 µm. N-nucleus, Nu- nucleolus
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4219776&req=5

pone-0111780-g002: Double labelling of poly(A) RNA and the PANA antigen in the chromocentric (Lupinus) (A–C) and reticular (Allium) (D–F) nuclei of root cells.In the nucleoplasm of both species, poly(A) RNA is present in nuclear structures (arrows) and does not colocalise with speckles. Representative examples of Pearson correlation coefficients for weak and non-colocalisation of poly(A) mRNA with the PANA antigen in Lupinus luteus and Allium cepa cells (G). A scale bar representing 2 µm is shown. The percentages of weak and non-colocalisation of poly(A) RNA-rich bodies with the PANA antigen are indicated by the Pearson correlation coefficient (H). Error bars represent standard error. Double labelling of poly(A) RNA and U2 snRNA in Lupinus (I–K) and Allium (L–N) cells. Accumulation of poly(A) RNA in nuclear bodies rich in U2 snRNA (arrows). Bar, 5 µm. N-nucleus, Nu- nucleolus
Mentions: We have shown that in the cells of plant roots, a considerably larger amount of poly(A) RNA is present in the nucleus than in the cytoplasm (up to more than 80% in lupine) (Figure 1). In the nucleoplasm, the signal forms clusters of different sizes, in both chromocentric (Lupinus luteus) (Figure 2A, C) and reticular (Allium cepa) nuclei (Figure 2D, F). Some of these clusters resemble nuclear bodies in terms of their shape and distribution. In lupine, the nuclear bodies enriched in poly(A) mRNA were more numerous and smaller in meristematic cells, whereas there were isolated but larger examples observed in differentiated cells of the roots (Figure S1A, B). These round clusters were observed in 87% of lupine root cells. Quantitative measurements have shown that in the meristematic cells of lupine plants, approximately 3.5% of the nuclear pool of the poly(A) RNA occurs in structures resembling nuclear bodies (Figure 1).

Bottom Line: In both types of nuclei, the amount of poly(A) RNA was much greater in the nucleus than in the cytoplasm.In addition, it was demonstrated that accumulation of poly(A) RNA occurs in the nuclei and CBs of hypoxia-treated cells.Our findings indicated that CBs may be involved in the later stages of poly(A) RNA metabolism, playing a role storage or retention.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT
The localisation of poly(A) RNA in plant cells containing either reticular (Allium cepa) or chromocentric (Lupinus luteus, Arabidopsis thaliana) nuclei was studied through in situ hybridisation. In both types of nuclei, the amount of poly(A) RNA was much greater in the nucleus than in the cytoplasm. In the nuclei, poly(A) RNA was present in structures resembling nuclear bodies. The molecular composition as well as the characteristic ultrastructure of the bodies containing poly(A) RNA demonstrated that they were Cajal bodies. We showed that some poly(A) RNAs in Cajal bodies code for proteins. However, examination of the localisation of active RNA polymerase II and in situ run-on transcription assays both demonstrated that CBs are not sites of transcription and that BrU-containing RNA accumulates in these structures long after synthesis. In addition, it was demonstrated that accumulation of poly(A) RNA occurs in the nuclei and CBs of hypoxia-treated cells. Our findings indicated that CBs may be involved in the later stages of poly(A) RNA metabolism, playing a role storage or retention.

Show MeSH