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Chemically defined diet alters the protective properties of fructo-oligosaccharides and isomalto-oligosaccharides in HLA-B27 transgenic rats.

Koleva P, Ketabi A, Valcheva R, Gänzle MG, Dieleman LA - PLoS ONE (2014)

Bottom Line: Bifidobacteria and Enterobacteriaceae were stimulated by FOS, whereas copy numbers of Clostridium cluster IV were decreased.In addition, higher concentrations of total short-chain fatty acids (SCFA) were observed in cecal contents of rats on rat chow compared to the chemically defined diet.Intestinal inflammation was positively correlated to protein fermentation and negatively correlated with carbohydrate fermentation in the large intestine.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada; Centre of Excellence for Gastrointestinal Inflammation and Immunity Research, University of Alberta, Edmonton, Canada.

ABSTRACT
Non-digestible oligosaccharides (NDO) were shown to reduce inflammation in experimental colitis, but it remains unclear whether microbiota changes mediate their colitis-modulating effects. This study assessed intestinal microbiota and intestinal inflammation after feeding chemically defined AIN-76A or rat chow diets, with or without supplementation with 8 g/kg body weight of fructo-oligosaccharides (FOS) or isomalto-oligosaccharides (IMO). The study used HLA-B27 transgenic rats, a validated model of inflammatory bowel disease (IBD), in a factorial design with 6 treatment groups. Intestinal inflammation and intestinal microbiota were analysed after 12 weeks of treatment. FOS and IMO reduced colitis in animals fed rat chow, but exhibited no anti-inflammatory effect when added to AIN-76A diets. Both NDO induced specific but divergent microbiota changes. Bifidobacteria and Enterobacteriaceae were stimulated by FOS, whereas copy numbers of Clostridium cluster IV were decreased. In addition, higher concentrations of total short-chain fatty acids (SCFA) were observed in cecal contents of rats on rat chow compared to the chemically defined diet. AIN-76A increased the relative proportions of propionate, iso-butyrate, valerate and iso-valerate irrespective of the oligosaccharide treatment. The SCFA composition, particularly the relative concentration of iso-butyrate, valerate and iso-valerate, was associated (P ≤ 0.004 and r ≥ 0.4) with increased colitis and IL-1 β concentration of the cecal mucosa. This study demonstrated that the protective effects of fibres on colitis development depend on the diet. Although diets modified specific cecal microbiota, our study indicates that these changes were not associated with colitis reduction. Intestinal inflammation was positively correlated to protein fermentation and negatively correlated with carbohydrate fermentation in the large intestine.

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Effects of diets and fibre additives on SCFA concentration in cecal contents.Samples were collected at the end point of the fibre treatment of HLA-B27 transgenic rats. Vertical bars show concentration of the respective SCFA related to the concentration of total SCFA and values are expressed as means ± SEM, n = 6 and n = 8 for rat chow and AIN-76A diet, respectively. Values that do not share a common superscript differ significantly (P<0.05, Bonferroni adjustment). A – acetate; B – propionate; C – butyrate; D – isobutyrate; E – valerate; F – isovalerate. Total SCFA concentrations were 220±23, 239±11 and 183±17 µmol/g for control, IMO, and FOS groups, respectively in digesta from treatment groups on rat chow and 118±11, 195±17 and 132±10 µmol/g for control, IMO, and FOS groups, respectively, in digesta from treatment groups on AIN-76.
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pone-0111717-g004: Effects of diets and fibre additives on SCFA concentration in cecal contents.Samples were collected at the end point of the fibre treatment of HLA-B27 transgenic rats. Vertical bars show concentration of the respective SCFA related to the concentration of total SCFA and values are expressed as means ± SEM, n = 6 and n = 8 for rat chow and AIN-76A diet, respectively. Values that do not share a common superscript differ significantly (P<0.05, Bonferroni adjustment). A – acetate; B – propionate; C – butyrate; D – isobutyrate; E – valerate; F – isovalerate. Total SCFA concentrations were 220±23, 239±11 and 183±17 µmol/g for control, IMO, and FOS groups, respectively in digesta from treatment groups on rat chow and 118±11, 195±17 and 132±10 µmol/g for control, IMO, and FOS groups, respectively, in digesta from treatment groups on AIN-76.

Mentions: To assess the effect of diet and fibre supplementation on production of microbial metabolites, SCFA were quantified in the cecum. Total SCFA concentrations (220±23, 239±11 and 183±17 µmol/g, for control, IMO, and FOS groups, respectively) in digesta from treatment groups on rat chow did not differ significantly between each other. However, supplementation of AIN-76A with IMO resulted in significantly higher SCFA concentrations (195±17 µmol/g) in comparison with the control and FOS groups (118±11 and 132±10 µmol/g; P = 0.001 and P = 0.0001, respectively). The relative concentration of acetate was significantly increased in IMO- and FOS-treated rats versus control group on rat chow (Figure 4A). FOS supplementation also increased relative acetate concentrations in rats fed AIN-76A (Figure 4A). Proportions of propionate and iso-butyrate showed little variation between diets (Fig. 4B and 4D). Valerate and isovalerate were reduced in comparison with control animals when FOS was included in the AIN-76A diet (Figure 4E and 4F).


Chemically defined diet alters the protective properties of fructo-oligosaccharides and isomalto-oligosaccharides in HLA-B27 transgenic rats.

Koleva P, Ketabi A, Valcheva R, Gänzle MG, Dieleman LA - PLoS ONE (2014)

Effects of diets and fibre additives on SCFA concentration in cecal contents.Samples were collected at the end point of the fibre treatment of HLA-B27 transgenic rats. Vertical bars show concentration of the respective SCFA related to the concentration of total SCFA and values are expressed as means ± SEM, n = 6 and n = 8 for rat chow and AIN-76A diet, respectively. Values that do not share a common superscript differ significantly (P<0.05, Bonferroni adjustment). A – acetate; B – propionate; C – butyrate; D – isobutyrate; E – valerate; F – isovalerate. Total SCFA concentrations were 220±23, 239±11 and 183±17 µmol/g for control, IMO, and FOS groups, respectively in digesta from treatment groups on rat chow and 118±11, 195±17 and 132±10 µmol/g for control, IMO, and FOS groups, respectively, in digesta from treatment groups on AIN-76.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219767&req=5

pone-0111717-g004: Effects of diets and fibre additives on SCFA concentration in cecal contents.Samples were collected at the end point of the fibre treatment of HLA-B27 transgenic rats. Vertical bars show concentration of the respective SCFA related to the concentration of total SCFA and values are expressed as means ± SEM, n = 6 and n = 8 for rat chow and AIN-76A diet, respectively. Values that do not share a common superscript differ significantly (P<0.05, Bonferroni adjustment). A – acetate; B – propionate; C – butyrate; D – isobutyrate; E – valerate; F – isovalerate. Total SCFA concentrations were 220±23, 239±11 and 183±17 µmol/g for control, IMO, and FOS groups, respectively in digesta from treatment groups on rat chow and 118±11, 195±17 and 132±10 µmol/g for control, IMO, and FOS groups, respectively, in digesta from treatment groups on AIN-76.
Mentions: To assess the effect of diet and fibre supplementation on production of microbial metabolites, SCFA were quantified in the cecum. Total SCFA concentrations (220±23, 239±11 and 183±17 µmol/g, for control, IMO, and FOS groups, respectively) in digesta from treatment groups on rat chow did not differ significantly between each other. However, supplementation of AIN-76A with IMO resulted in significantly higher SCFA concentrations (195±17 µmol/g) in comparison with the control and FOS groups (118±11 and 132±10 µmol/g; P = 0.001 and P = 0.0001, respectively). The relative concentration of acetate was significantly increased in IMO- and FOS-treated rats versus control group on rat chow (Figure 4A). FOS supplementation also increased relative acetate concentrations in rats fed AIN-76A (Figure 4A). Proportions of propionate and iso-butyrate showed little variation between diets (Fig. 4B and 4D). Valerate and isovalerate were reduced in comparison with control animals when FOS was included in the AIN-76A diet (Figure 4E and 4F).

Bottom Line: Bifidobacteria and Enterobacteriaceae were stimulated by FOS, whereas copy numbers of Clostridium cluster IV were decreased.In addition, higher concentrations of total short-chain fatty acids (SCFA) were observed in cecal contents of rats on rat chow compared to the chemically defined diet.Intestinal inflammation was positively correlated to protein fermentation and negatively correlated with carbohydrate fermentation in the large intestine.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada; Centre of Excellence for Gastrointestinal Inflammation and Immunity Research, University of Alberta, Edmonton, Canada.

ABSTRACT
Non-digestible oligosaccharides (NDO) were shown to reduce inflammation in experimental colitis, but it remains unclear whether microbiota changes mediate their colitis-modulating effects. This study assessed intestinal microbiota and intestinal inflammation after feeding chemically defined AIN-76A or rat chow diets, with or without supplementation with 8 g/kg body weight of fructo-oligosaccharides (FOS) or isomalto-oligosaccharides (IMO). The study used HLA-B27 transgenic rats, a validated model of inflammatory bowel disease (IBD), in a factorial design with 6 treatment groups. Intestinal inflammation and intestinal microbiota were analysed after 12 weeks of treatment. FOS and IMO reduced colitis in animals fed rat chow, but exhibited no anti-inflammatory effect when added to AIN-76A diets. Both NDO induced specific but divergent microbiota changes. Bifidobacteria and Enterobacteriaceae were stimulated by FOS, whereas copy numbers of Clostridium cluster IV were decreased. In addition, higher concentrations of total short-chain fatty acids (SCFA) were observed in cecal contents of rats on rat chow compared to the chemically defined diet. AIN-76A increased the relative proportions of propionate, iso-butyrate, valerate and iso-valerate irrespective of the oligosaccharide treatment. The SCFA composition, particularly the relative concentration of iso-butyrate, valerate and iso-valerate, was associated (P ≤ 0.004 and r ≥ 0.4) with increased colitis and IL-1 β concentration of the cecal mucosa. This study demonstrated that the protective effects of fibres on colitis development depend on the diet. Although diets modified specific cecal microbiota, our study indicates that these changes were not associated with colitis reduction. Intestinal inflammation was positively correlated to protein fermentation and negatively correlated with carbohydrate fermentation in the large intestine.

Show MeSH
Related in: MedlinePlus