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A novel fungal hyperparasite of Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust.

Zhan G, Tian Y, Wang F, Chen X, Guo J, Jiao M, Huang L, Kang Z - PLoS ONE (2014)

Bottom Line: Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis.Here, we report a novel hyperparasite on Pst.The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

ABSTRACT
Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis. Here, we report a novel hyperparasite on Pst. This hyperparasitic fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries based on morphological characteristics observed by light and scanning electron microscopy together with molecular data. The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

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A neighbor-joining tree constructed using the sequences of ITS,EF and ACT to determine the phylogenetic relationship of the hyperparasitic fungal strain CGMCC 7.175 (bold and marked with *) isolated from uredinia of Pst with other accessions of Cladosporium spp. in GenBank.The values at braches are percentages from 1,000 bootstrap replications.
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pone-0111484-g006: A neighbor-joining tree constructed using the sequences of ITS,EF and ACT to determine the phylogenetic relationship of the hyperparasitic fungal strain CGMCC 7.175 (bold and marked with *) isolated from uredinia of Pst with other accessions of Cladosporium spp. in GenBank.The values at braches are percentages from 1,000 bootstrap replications.

Mentions: The genomic sequences of internal transcribed spacer (ITS) region, actin (ACT) and translation elongation factor 1-α (EF) supported the identification of the fungal strain as C. cladosporioides. Phylogenetic analysis with these sequences (GenBank accession numbers: KJ598781 for ITS, KM281944 for ACT and KM281945 for EF) clustered the fungus and eleven other known Cladosporium spp. into four groups, and CGMCC 7.175 clustered with six other isolates of C. cladosporioides in accordance with combined ITS, ACT and EF sequences (Figure 6). The C. cladosporioides group was divided into two subclades. CBS143.35, CBS144.35, CBS145.35 and CBS101367 formed one subclade, while CPC14018, CPC14244 and KJ598781 formed the other. Our novel strain (CGMCC 7.175) was found to be most closely related to CPC14244.


A novel fungal hyperparasite of Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust.

Zhan G, Tian Y, Wang F, Chen X, Guo J, Jiao M, Huang L, Kang Z - PLoS ONE (2014)

A neighbor-joining tree constructed using the sequences of ITS,EF and ACT to determine the phylogenetic relationship of the hyperparasitic fungal strain CGMCC 7.175 (bold and marked with *) isolated from uredinia of Pst with other accessions of Cladosporium spp. in GenBank.The values at braches are percentages from 1,000 bootstrap replications.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4219752&req=5

pone-0111484-g006: A neighbor-joining tree constructed using the sequences of ITS,EF and ACT to determine the phylogenetic relationship of the hyperparasitic fungal strain CGMCC 7.175 (bold and marked with *) isolated from uredinia of Pst with other accessions of Cladosporium spp. in GenBank.The values at braches are percentages from 1,000 bootstrap replications.
Mentions: The genomic sequences of internal transcribed spacer (ITS) region, actin (ACT) and translation elongation factor 1-α (EF) supported the identification of the fungal strain as C. cladosporioides. Phylogenetic analysis with these sequences (GenBank accession numbers: KJ598781 for ITS, KM281944 for ACT and KM281945 for EF) clustered the fungus and eleven other known Cladosporium spp. into four groups, and CGMCC 7.175 clustered with six other isolates of C. cladosporioides in accordance with combined ITS, ACT and EF sequences (Figure 6). The C. cladosporioides group was divided into two subclades. CBS143.35, CBS144.35, CBS145.35 and CBS101367 formed one subclade, while CPC14018, CPC14244 and KJ598781 formed the other. Our novel strain (CGMCC 7.175) was found to be most closely related to CPC14244.

Bottom Line: Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis.Here, we report a novel hyperparasite on Pst.The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

ABSTRACT
Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis. Here, we report a novel hyperparasite on Pst. This hyperparasitic fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries based on morphological characteristics observed by light and scanning electron microscopy together with molecular data. The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

Show MeSH
Related in: MedlinePlus