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A novel fungal hyperparasite of Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust.

Zhan G, Tian Y, Wang F, Chen X, Guo J, Jiao M, Huang L, Kang Z - PLoS ONE (2014)

Bottom Line: Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis.Here, we report a novel hyperparasite on Pst.The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

ABSTRACT
Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis. Here, we report a novel hyperparasite on Pst. This hyperparasitic fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries based on morphological characteristics observed by light and scanning electron microscopy together with molecular data. The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

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Related in: MedlinePlus

Morphological features of Cladosporium cladosporioides cultured on MEA medium.A: Fungal colonies on MEA medium, B: Branched ramoconidia and chains of conidia, C: Ramoconidia and dense conidia on the tip of a conidiophore, D: Oval to spherical conidia and separated ramoconidia with a dark scar at one end or both ends.
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pone-0111484-g002: Morphological features of Cladosporium cladosporioides cultured on MEA medium.A: Fungal colonies on MEA medium, B: Branched ramoconidia and chains of conidia, C: Ramoconidia and dense conidia on the tip of a conidiophore, D: Oval to spherical conidia and separated ramoconidia with a dark scar at one end or both ends.

Mentions: The hyperparasitic fungus isolated from Pst uredinia grew rapidly on malt extract agar (MEA) medium, forming olive-gray to olive-green colonies with gray aerial mycelia (Figure 2A). By light microscopy, hyphae were found to be unbranched or only occasionally branched, and 1–4 µm wide. In branched hyphae, the branch sites were not associated with swollen or constricted regions. Conidiophores were solitary, straight or slightly curved, without nodules, and occasionally geniculate, 40–300 µm×3–4 µm. They were unbranched or occasionally branched, had a tapering tip, and the base was sometimes swollen (Figure 2 B–D). Ramoconidia were usually produced in groups of three at the tips of conidiophores. They were long and cylindrical (12–55 µm×3–5 µm), straight or slightly curved with a fine toothed projection. Conidia were formed in chains, nearly spherical, oval or lemon-shaped, 3–6 µm×2–3 µm. The more detailed morphological characteristics of conidia, ramoconidia, conidiophores and mycelia obtained by observations with a SEM are illustrated in Figure 3 A–F. Based on the morphological characteristics, the filamentous fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries. The C. cladosporioides culture was deposited in China General Microbiological Culture Collection Center (CGMCC), Beijing, China (http://www.cgmcc.net) with an accession number of CGMCC 7.175.


A novel fungal hyperparasite of Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust.

Zhan G, Tian Y, Wang F, Chen X, Guo J, Jiao M, Huang L, Kang Z - PLoS ONE (2014)

Morphological features of Cladosporium cladosporioides cultured on MEA medium.A: Fungal colonies on MEA medium, B: Branched ramoconidia and chains of conidia, C: Ramoconidia and dense conidia on the tip of a conidiophore, D: Oval to spherical conidia and separated ramoconidia with a dark scar at one end or both ends.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4219752&req=5

pone-0111484-g002: Morphological features of Cladosporium cladosporioides cultured on MEA medium.A: Fungal colonies on MEA medium, B: Branched ramoconidia and chains of conidia, C: Ramoconidia and dense conidia on the tip of a conidiophore, D: Oval to spherical conidia and separated ramoconidia with a dark scar at one end or both ends.
Mentions: The hyperparasitic fungus isolated from Pst uredinia grew rapidly on malt extract agar (MEA) medium, forming olive-gray to olive-green colonies with gray aerial mycelia (Figure 2A). By light microscopy, hyphae were found to be unbranched or only occasionally branched, and 1–4 µm wide. In branched hyphae, the branch sites were not associated with swollen or constricted regions. Conidiophores were solitary, straight or slightly curved, without nodules, and occasionally geniculate, 40–300 µm×3–4 µm. They were unbranched or occasionally branched, had a tapering tip, and the base was sometimes swollen (Figure 2 B–D). Ramoconidia were usually produced in groups of three at the tips of conidiophores. They were long and cylindrical (12–55 µm×3–5 µm), straight or slightly curved with a fine toothed projection. Conidia were formed in chains, nearly spherical, oval or lemon-shaped, 3–6 µm×2–3 µm. The more detailed morphological characteristics of conidia, ramoconidia, conidiophores and mycelia obtained by observations with a SEM are illustrated in Figure 3 A–F. Based on the morphological characteristics, the filamentous fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries. The C. cladosporioides culture was deposited in China General Microbiological Culture Collection Center (CGMCC), Beijing, China (http://www.cgmcc.net) with an accession number of CGMCC 7.175.

Bottom Line: Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis.Here, we report a novel hyperparasite on Pst.The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

ABSTRACT
Puccinia striiformis f. sp. tritici (Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis. Here, we report a novel hyperparasite on Pst. This hyperparasitic fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries based on morphological characteristics observed by light and scanning electron microscopy together with molecular data. The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust.

Show MeSH
Related in: MedlinePlus