Limits...
Meningiomas: a comparative study of 68Ga-DOTATOC, 68Ga-DOTANOC and 68Ga-DOTATATE for molecular imaging in mice.

Soto-Montenegro ML, Peña-Zalbidea S, Mateos-Pérez JM, Oteo M, Romero E, Morcillo MÁ, Desco M - PLoS ONE (2014)

Bottom Line: No significant differences between tracers were found for SUVmax in tumor or muscle.No differences were found in the T/L SUV ratio between (68)Ga-DOTATATE and (68)Ga-DOTATOC, both of which had a higher fraction than (68)Ga-DOTANOC.The T/M SUV ratio was significantly higher with (68)Ga-DOTATATE than with (68)Ga-DOTATOC and (68)Ga-DOTANOC.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Medicina y Cirugía Experimental, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Madrid, Spain.

ABSTRACT

Purpose: The goal of this study was to compare the tumor uptake kinetics and diagnostic value of three (68)Ga-DOTA-labeled somatostatin analogues ((68)Ga-DOTATOC, (68)Ga-DOTANOC, and (68)Ga-DOTATATE) using PET/CT in a murine model with subcutaneous meningioma xenografts.

Methods: The experiment was performed with 16 male NUDE NU/NU mice bearing xenografts of a human meningioma cell line (CH-157MN). (68)Ga-DOTATOC, (68)Ga-DOTANOC, and (68)Ga-DOTATATE were produced in a FASTLab automated platform. Imaging was performed on an Argus small-animal PET/CT scanner. The SUVmax of the liver and muscle, and the tumor-to-liver (T/L) and tumor-to-muscle (T/M) SUV ratios were computed. Kinetic analysis was performed using Logan graphical analysis for a two-tissue reversible compartmental model, and the volume of distribution (Vt) was determined.

Results: Hepatic SUVmax and Vt were significantly higher with (68)Ga-DOTANOC than with (68)Ga-DOTATOC and (68)Ga-DOTATATE. No significant differences between tracers were found for SUVmax in tumor or muscle. No differences were found in the T/L SUV ratio between (68)Ga-DOTATATE and (68)Ga-DOTATOC, both of which had a higher fraction than (68)Ga-DOTANOC. The T/M SUV ratio was significantly higher with (68)Ga-DOTATATE than with (68)Ga-DOTATOC and (68)Ga-DOTANOC. The Vt for tumor was higher with (68)Ga-DOTATATE than with (68)Ga-DOTANOC and relatively similar to that of (68)Ga-DOTATOC.

Conclusions: This study demonstrates, for the first time, the ability of the three radiolabeled somatostatin analogues tested to image a human meningioma cell line. Although Vt was relatively similar with (68)Ga-DOTATATE and (68)Ga-DOTATOC, uptake was higher with (68)Ga-DOTATATE in the tumor than with (68)Ga-DOTANOC and (68)Ga-DOTATOC, suggesting a higher diagnostic value of (68)Ga-DOTATATE for detecting meningiomas.

Show MeSH

Related in: MedlinePlus

Experimental protocol.Animals were imaged with 68Ga-DOTATOC (white line), 68Ga-DOTANOC (gray line) and/or 68Ga-DOTATATE (dotted line). Half of the animals imaged with 68Ga-DOTATOC were also imaged with the other two radiotracers in alternate days with respect to 68Ga-DOTATOC (n represents the number of animals).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4219730&req=5

pone-0111624-g001: Experimental protocol.Animals were imaged with 68Ga-DOTATOC (white line), 68Ga-DOTANOC (gray line) and/or 68Ga-DOTATATE (dotted line). Half of the animals imaged with 68Ga-DOTATOC were also imaged with the other two radiotracers in alternate days with respect to 68Ga-DOTATOC (n represents the number of animals).

Mentions: Animals were scanned on a small-animal PET scanner (ARGUS PET-CT, SEDECAL, Madrid, Spain) under anesthesia (isoflurane, 3% induction and 1.5% maintenance in 100% O2). Imaging was performed 7–28 days after tumor cell inoculation. Animals were imaged over four consecutive weeks (at days 7, 14, 21 & 28 after cell inoculation) with one radiotracer (n = 8 with 68Ga-DOTATOC; n = 4 with 68Ga-DOTANOC and n = 4 with 68Ga-DOTATATE). Half of the animals imaged with 68Ga-DOTATOC were also imaged with the other two radiotracers in alternate days with respect to 68Ga-DOTATOC (Figure 1). Overall, n values for the PET study were: 27 for 68Ga-DOTATOC, 20 for 68Ga-DOTANOC and 21 for 68Ga-DOTATATE.


Meningiomas: a comparative study of 68Ga-DOTATOC, 68Ga-DOTANOC and 68Ga-DOTATATE for molecular imaging in mice.

Soto-Montenegro ML, Peña-Zalbidea S, Mateos-Pérez JM, Oteo M, Romero E, Morcillo MÁ, Desco M - PLoS ONE (2014)

Experimental protocol.Animals were imaged with 68Ga-DOTATOC (white line), 68Ga-DOTANOC (gray line) and/or 68Ga-DOTATATE (dotted line). Half of the animals imaged with 68Ga-DOTATOC were also imaged with the other two radiotracers in alternate days with respect to 68Ga-DOTATOC (n represents the number of animals).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219730&req=5

pone-0111624-g001: Experimental protocol.Animals were imaged with 68Ga-DOTATOC (white line), 68Ga-DOTANOC (gray line) and/or 68Ga-DOTATATE (dotted line). Half of the animals imaged with 68Ga-DOTATOC were also imaged with the other two radiotracers in alternate days with respect to 68Ga-DOTATOC (n represents the number of animals).
Mentions: Animals were scanned on a small-animal PET scanner (ARGUS PET-CT, SEDECAL, Madrid, Spain) under anesthesia (isoflurane, 3% induction and 1.5% maintenance in 100% O2). Imaging was performed 7–28 days after tumor cell inoculation. Animals were imaged over four consecutive weeks (at days 7, 14, 21 & 28 after cell inoculation) with one radiotracer (n = 8 with 68Ga-DOTATOC; n = 4 with 68Ga-DOTANOC and n = 4 with 68Ga-DOTATATE). Half of the animals imaged with 68Ga-DOTATOC were also imaged with the other two radiotracers in alternate days with respect to 68Ga-DOTATOC (Figure 1). Overall, n values for the PET study were: 27 for 68Ga-DOTATOC, 20 for 68Ga-DOTANOC and 21 for 68Ga-DOTATATE.

Bottom Line: No significant differences between tracers were found for SUVmax in tumor or muscle.No differences were found in the T/L SUV ratio between (68)Ga-DOTATATE and (68)Ga-DOTATOC, both of which had a higher fraction than (68)Ga-DOTANOC.The T/M SUV ratio was significantly higher with (68)Ga-DOTATATE than with (68)Ga-DOTATOC and (68)Ga-DOTANOC.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Medicina y Cirugía Experimental, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Madrid, Spain.

ABSTRACT

Purpose: The goal of this study was to compare the tumor uptake kinetics and diagnostic value of three (68)Ga-DOTA-labeled somatostatin analogues ((68)Ga-DOTATOC, (68)Ga-DOTANOC, and (68)Ga-DOTATATE) using PET/CT in a murine model with subcutaneous meningioma xenografts.

Methods: The experiment was performed with 16 male NUDE NU/NU mice bearing xenografts of a human meningioma cell line (CH-157MN). (68)Ga-DOTATOC, (68)Ga-DOTANOC, and (68)Ga-DOTATATE were produced in a FASTLab automated platform. Imaging was performed on an Argus small-animal PET/CT scanner. The SUVmax of the liver and muscle, and the tumor-to-liver (T/L) and tumor-to-muscle (T/M) SUV ratios were computed. Kinetic analysis was performed using Logan graphical analysis for a two-tissue reversible compartmental model, and the volume of distribution (Vt) was determined.

Results: Hepatic SUVmax and Vt were significantly higher with (68)Ga-DOTANOC than with (68)Ga-DOTATOC and (68)Ga-DOTATATE. No significant differences between tracers were found for SUVmax in tumor or muscle. No differences were found in the T/L SUV ratio between (68)Ga-DOTATATE and (68)Ga-DOTATOC, both of which had a higher fraction than (68)Ga-DOTANOC. The T/M SUV ratio was significantly higher with (68)Ga-DOTATATE than with (68)Ga-DOTATOC and (68)Ga-DOTANOC. The Vt for tumor was higher with (68)Ga-DOTATATE than with (68)Ga-DOTANOC and relatively similar to that of (68)Ga-DOTATOC.

Conclusions: This study demonstrates, for the first time, the ability of the three radiolabeled somatostatin analogues tested to image a human meningioma cell line. Although Vt was relatively similar with (68)Ga-DOTATATE and (68)Ga-DOTATOC, uptake was higher with (68)Ga-DOTATATE in the tumor than with (68)Ga-DOTANOC and (68)Ga-DOTATOC, suggesting a higher diagnostic value of (68)Ga-DOTATATE for detecting meningiomas.

Show MeSH
Related in: MedlinePlus