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60-Day chronic exposure to low concentrations of HgCl2 impairs sperm quality: hormonal imbalance and oxidative stress as potential routes for reproductive dysfunction in rats.

Martinez CS, Torres JG, Peçanha FM, Anselmo-Franci JA, Vassallo DV, Salaices M, Alonso MJ, Wiggers GA - PLoS ONE (2014)

Bottom Line: Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities.Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs.The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Biochemistry, Universidade Federal do Pampa, Uruguaiana, Rio Grande do Sul, Brazil.

ABSTRACT
Mercury is a toxic and bio-accumulative heavy metal of global concern. While good deals of research have been conducted on the toxic effects of mercury, little is known about the mechanisms involved in the pathogenesis of male reproductive dysfunction induced by mercury. Therefore, the purpose of this study was to assess the effects and underlying mechanisms of chronic mercury exposure at low levels on male reproductive system of rats. Three-month-old male Wistar rats were divided into two groups and treated for 60 days with saline (i.m., Control) and HgCl2 (i.m. 1st dose: 4.6 µg/kg, subsequent doses 0.07 µg/kg/day). We analyzed sperm parameters, hormonal levels and biomarkers of oxidative stress in testis, epididymis, prostate and vas deferens. Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities. Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs. Our data demonstrate that 60-day chronic exposure to low concentrations of HgCl2 impairs sperm quality and promotes hormonal imbalance. The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

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Effect of treatment for 60 days at low concentrations of HgCl2 on serum hormonal levels.(A) Luteinizing hormone (LH) and testosterone levels (B). Data are expressed as mean ± SEM (n = 10). *p<0.05 (Student's t-test).
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pone-0111202-g006: Effect of treatment for 60 days at low concentrations of HgCl2 on serum hormonal levels.(A) Luteinizing hormone (LH) and testosterone levels (B). Data are expressed as mean ± SEM (n = 10). *p<0.05 (Student's t-test).

Mentions: There was a significant reduction in serum levels of LH in the mercury treated rats when compared with the control group (Figure 6A), with no differences in testosterone levels after mercury exposition (Figure 6B).


60-Day chronic exposure to low concentrations of HgCl2 impairs sperm quality: hormonal imbalance and oxidative stress as potential routes for reproductive dysfunction in rats.

Martinez CS, Torres JG, Peçanha FM, Anselmo-Franci JA, Vassallo DV, Salaices M, Alonso MJ, Wiggers GA - PLoS ONE (2014)

Effect of treatment for 60 days at low concentrations of HgCl2 on serum hormonal levels.(A) Luteinizing hormone (LH) and testosterone levels (B). Data are expressed as mean ± SEM (n = 10). *p<0.05 (Student's t-test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219708&req=5

pone-0111202-g006: Effect of treatment for 60 days at low concentrations of HgCl2 on serum hormonal levels.(A) Luteinizing hormone (LH) and testosterone levels (B). Data are expressed as mean ± SEM (n = 10). *p<0.05 (Student's t-test).
Mentions: There was a significant reduction in serum levels of LH in the mercury treated rats when compared with the control group (Figure 6A), with no differences in testosterone levels after mercury exposition (Figure 6B).

Bottom Line: Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities.Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs.The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Biochemistry, Universidade Federal do Pampa, Uruguaiana, Rio Grande do Sul, Brazil.

ABSTRACT
Mercury is a toxic and bio-accumulative heavy metal of global concern. While good deals of research have been conducted on the toxic effects of mercury, little is known about the mechanisms involved in the pathogenesis of male reproductive dysfunction induced by mercury. Therefore, the purpose of this study was to assess the effects and underlying mechanisms of chronic mercury exposure at low levels on male reproductive system of rats. Three-month-old male Wistar rats were divided into two groups and treated for 60 days with saline (i.m., Control) and HgCl2 (i.m. 1st dose: 4.6 µg/kg, subsequent doses 0.07 µg/kg/day). We analyzed sperm parameters, hormonal levels and biomarkers of oxidative stress in testis, epididymis, prostate and vas deferens. Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities. Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs. Our data demonstrate that 60-day chronic exposure to low concentrations of HgCl2 impairs sperm quality and promotes hormonal imbalance. The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

Show MeSH
Related in: MedlinePlus