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60-Day chronic exposure to low concentrations of HgCl2 impairs sperm quality: hormonal imbalance and oxidative stress as potential routes for reproductive dysfunction in rats.

Martinez CS, Torres JG, Peçanha FM, Anselmo-Franci JA, Vassallo DV, Salaices M, Alonso MJ, Wiggers GA - PLoS ONE (2014)

Bottom Line: Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities.Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs.The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Biochemistry, Universidade Federal do Pampa, Uruguaiana, Rio Grande do Sul, Brazil.

ABSTRACT
Mercury is a toxic and bio-accumulative heavy metal of global concern. While good deals of research have been conducted on the toxic effects of mercury, little is known about the mechanisms involved in the pathogenesis of male reproductive dysfunction induced by mercury. Therefore, the purpose of this study was to assess the effects and underlying mechanisms of chronic mercury exposure at low levels on male reproductive system of rats. Three-month-old male Wistar rats were divided into two groups and treated for 60 days with saline (i.m., Control) and HgCl2 (i.m. 1st dose: 4.6 µg/kg, subsequent doses 0.07 µg/kg/day). We analyzed sperm parameters, hormonal levels and biomarkers of oxidative stress in testis, epididymis, prostate and vas deferens. Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities. Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs. Our data demonstrate that 60-day chronic exposure to low concentrations of HgCl2 impairs sperm quality and promotes hormonal imbalance. The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

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Effect of treatment for 60 days at low concentrations of HgCl2 on catalase activity.Values of catalase activity on testis (A), epididymis (B), prostate (C) and vas deferens (D). Data are expressed as mean ± SEM (n = 6). ** p<0.01, *p<0.05 (Student's t-test).
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pone-0111202-g005: Effect of treatment for 60 days at low concentrations of HgCl2 on catalase activity.Values of catalase activity on testis (A), epididymis (B), prostate (C) and vas deferens (D). Data are expressed as mean ± SEM (n = 6). ** p<0.01, *p<0.05 (Student's t-test).

Mentions: The mercury exposure for 60 days decreased the enzymatic activity of SOD in testis, epididymis and prostate (Figure 4A, B, C), without change in rats vas deferens (Figure 4D). Moreover, the catalase activity after mercury treatment slightly decreased in epididymis and in a great extent in vas deferens of rats (Figure 5B, D); however, in testis and prostate there were not differences in the activity of this enzyme (Figure 5A, C).


60-Day chronic exposure to low concentrations of HgCl2 impairs sperm quality: hormonal imbalance and oxidative stress as potential routes for reproductive dysfunction in rats.

Martinez CS, Torres JG, Peçanha FM, Anselmo-Franci JA, Vassallo DV, Salaices M, Alonso MJ, Wiggers GA - PLoS ONE (2014)

Effect of treatment for 60 days at low concentrations of HgCl2 on catalase activity.Values of catalase activity on testis (A), epididymis (B), prostate (C) and vas deferens (D). Data are expressed as mean ± SEM (n = 6). ** p<0.01, *p<0.05 (Student's t-test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219708&req=5

pone-0111202-g005: Effect of treatment for 60 days at low concentrations of HgCl2 on catalase activity.Values of catalase activity on testis (A), epididymis (B), prostate (C) and vas deferens (D). Data are expressed as mean ± SEM (n = 6). ** p<0.01, *p<0.05 (Student's t-test).
Mentions: The mercury exposure for 60 days decreased the enzymatic activity of SOD in testis, epididymis and prostate (Figure 4A, B, C), without change in rats vas deferens (Figure 4D). Moreover, the catalase activity after mercury treatment slightly decreased in epididymis and in a great extent in vas deferens of rats (Figure 5B, D); however, in testis and prostate there were not differences in the activity of this enzyme (Figure 5A, C).

Bottom Line: Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities.Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs.The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Biochemistry, Universidade Federal do Pampa, Uruguaiana, Rio Grande do Sul, Brazil.

ABSTRACT
Mercury is a toxic and bio-accumulative heavy metal of global concern. While good deals of research have been conducted on the toxic effects of mercury, little is known about the mechanisms involved in the pathogenesis of male reproductive dysfunction induced by mercury. Therefore, the purpose of this study was to assess the effects and underlying mechanisms of chronic mercury exposure at low levels on male reproductive system of rats. Three-month-old male Wistar rats were divided into two groups and treated for 60 days with saline (i.m., Control) and HgCl2 (i.m. 1st dose: 4.6 µg/kg, subsequent doses 0.07 µg/kg/day). We analyzed sperm parameters, hormonal levels and biomarkers of oxidative stress in testis, epididymis, prostate and vas deferens. Mercury treatment decreased daily sperm production, count and motility and increased head and tail morphologic abnormalities. Moreover, mercury treatment decreased luteinizing hormone levels, increased lipid peroxidation on testis and decreased antioxidant enzymes activities (superoxide dismutase and catalase) on reproductive organs. Our data demonstrate that 60-day chronic exposure to low concentrations of HgCl2 impairs sperm quality and promotes hormonal imbalance. The raised oxidative stress seems to be a potential mechanism involved on male reproductive toxicity by mercury.

Show MeSH
Related in: MedlinePlus