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Stability of 35 biochemical and immunological routine tests after 10 hours storage and transport of human whole blood at 21°C.

Henriksen LO, Faber NR, Moller MF, Nexo E, Hansen AB - Scand. J. Clin. Lab. Invest. (2014)

Bottom Line: We observed no statistically significant bias and results within the goal for imprecision between baseline samples and 10-h samples for albumin, alkaline phosphatase, antitrypsin, bilirubin, creatinine, free triiodothyronine, γ-glutamyl transferase, haptoglobin, immunoglobulin G, lactate dehydrogenase, prostate specific antigen, total carbon dioxide, and urea.Alanine aminotransferase, amylase, C-reactive protein, calcium, cholesterol, creatine kinase, ferritin, free thyroxine, immunoglobulin A, immunoglobulin M, orosomucoid, sodium, transferrin, and triglycerides met goals for imprecision, though they showed a minor, but statistically significant bias in results after storage.We conclude that whole blood in lithium-heparin or serum tubes stored for 10 h at 21 ± 1°C, may be used for routine analysis without restrictions for all investigated analytes but folate and phosphate.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Biochemistry, Regional Hospital West Jutland , Herning and Holstebro , Denmark.

ABSTRACT

Background: Suitable procedures for transport of blood samples from general practitioners to hospital laboratories are requested. Here we explore routine testing on samples stored and transported as whole blood in lithium-heparin or serum tubes.

Methods: Blood samples were collected from 106 hospitalized patients, and analyzed on Architect c8000 or Advia Centaur XP for 35 analytes at base line, and after storage and transport of whole blood in lithium-heparin or serum tubes at 21 ± 1°C for 10 h. Bias and imprecision (representing variation from analysis and storage) were calculated from values at baseline and after storage, and differences tested by paired t-tests. Results were compared to goals set by the laboratory.

Results: We observed no statistically significant bias and results within the goal for imprecision between baseline samples and 10-h samples for albumin, alkaline phosphatase, antitrypsin, bilirubin, creatinine, free triiodothyronine, γ-glutamyl transferase, haptoglobin, immunoglobulin G, lactate dehydrogenase, prostate specific antigen, total carbon dioxide, and urea. Alanine aminotransferase, amylase, C-reactive protein, calcium, cholesterol, creatine kinase, ferritin, free thyroxine, immunoglobulin A, immunoglobulin M, orosomucoid, sodium, transferrin, and triglycerides met goals for imprecision, though they showed a minor, but statistically significant bias in results after storage. Cobalamin, folate, HDL-cholesterol, iron, phosphate, potassium, thyroid stimulating hormone and urate warranted concern, but only folate and phosphate showed deviations of clinical importance.

Conclusions: We conclude that whole blood in lithium-heparin or serum tubes stored for 10 h at 21 ± 1°C, may be used for routine analysis without restrictions for all investigated analytes but folate and phosphate.

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Related in: MedlinePlus

Bland-Altman plots of results obtained after storage of whole blood (in lithium-heparin or serum tubes) for 10 h prior to analysis as compared to results at baseline. Fraction: Ratio between baseline results and results at 10 h. Indicated on each figure is the line of identity, i.e. baseline/10 h = 1.0 (bold dashed line), the analytical CV (thin dashed line), goal-CV (full drawn thin line) and the reference interval (shaded area). Data for analytical- and goal-CVs are indicated for each analyte in Table II.
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Figure 2: Bland-Altman plots of results obtained after storage of whole blood (in lithium-heparin or serum tubes) for 10 h prior to analysis as compared to results at baseline. Fraction: Ratio between baseline results and results at 10 h. Indicated on each figure is the line of identity, i.e. baseline/10 h = 1.0 (bold dashed line), the analytical CV (thin dashed line), goal-CV (full drawn thin line) and the reference interval (shaded area). Data for analytical- and goal-CVs are indicated for each analyte in Table II.

Mentions: We also analyzed the results using fractional Bland-Altman plots, and present plots for B12, Fe, FOL, HDL-C, K, P, TSH, and URATE (Figure 2).


Stability of 35 biochemical and immunological routine tests after 10 hours storage and transport of human whole blood at 21°C.

Henriksen LO, Faber NR, Moller MF, Nexo E, Hansen AB - Scand. J. Clin. Lab. Invest. (2014)

Bland-Altman plots of results obtained after storage of whole blood (in lithium-heparin or serum tubes) for 10 h prior to analysis as compared to results at baseline. Fraction: Ratio between baseline results and results at 10 h. Indicated on each figure is the line of identity, i.e. baseline/10 h = 1.0 (bold dashed line), the analytical CV (thin dashed line), goal-CV (full drawn thin line) and the reference interval (shaded area). Data for analytical- and goal-CVs are indicated for each analyte in Table II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4219582&req=5

Figure 2: Bland-Altman plots of results obtained after storage of whole blood (in lithium-heparin or serum tubes) for 10 h prior to analysis as compared to results at baseline. Fraction: Ratio between baseline results and results at 10 h. Indicated on each figure is the line of identity, i.e. baseline/10 h = 1.0 (bold dashed line), the analytical CV (thin dashed line), goal-CV (full drawn thin line) and the reference interval (shaded area). Data for analytical- and goal-CVs are indicated for each analyte in Table II.
Mentions: We also analyzed the results using fractional Bland-Altman plots, and present plots for B12, Fe, FOL, HDL-C, K, P, TSH, and URATE (Figure 2).

Bottom Line: We observed no statistically significant bias and results within the goal for imprecision between baseline samples and 10-h samples for albumin, alkaline phosphatase, antitrypsin, bilirubin, creatinine, free triiodothyronine, γ-glutamyl transferase, haptoglobin, immunoglobulin G, lactate dehydrogenase, prostate specific antigen, total carbon dioxide, and urea.Alanine aminotransferase, amylase, C-reactive protein, calcium, cholesterol, creatine kinase, ferritin, free thyroxine, immunoglobulin A, immunoglobulin M, orosomucoid, sodium, transferrin, and triglycerides met goals for imprecision, though they showed a minor, but statistically significant bias in results after storage.We conclude that whole blood in lithium-heparin or serum tubes stored for 10 h at 21 ± 1°C, may be used for routine analysis without restrictions for all investigated analytes but folate and phosphate.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Biochemistry, Regional Hospital West Jutland , Herning and Holstebro , Denmark.

ABSTRACT

Background: Suitable procedures for transport of blood samples from general practitioners to hospital laboratories are requested. Here we explore routine testing on samples stored and transported as whole blood in lithium-heparin or serum tubes.

Methods: Blood samples were collected from 106 hospitalized patients, and analyzed on Architect c8000 or Advia Centaur XP for 35 analytes at base line, and after storage and transport of whole blood in lithium-heparin or serum tubes at 21 ± 1°C for 10 h. Bias and imprecision (representing variation from analysis and storage) were calculated from values at baseline and after storage, and differences tested by paired t-tests. Results were compared to goals set by the laboratory.

Results: We observed no statistically significant bias and results within the goal for imprecision between baseline samples and 10-h samples for albumin, alkaline phosphatase, antitrypsin, bilirubin, creatinine, free triiodothyronine, γ-glutamyl transferase, haptoglobin, immunoglobulin G, lactate dehydrogenase, prostate specific antigen, total carbon dioxide, and urea. Alanine aminotransferase, amylase, C-reactive protein, calcium, cholesterol, creatine kinase, ferritin, free thyroxine, immunoglobulin A, immunoglobulin M, orosomucoid, sodium, transferrin, and triglycerides met goals for imprecision, though they showed a minor, but statistically significant bias in results after storage. Cobalamin, folate, HDL-cholesterol, iron, phosphate, potassium, thyroid stimulating hormone and urate warranted concern, but only folate and phosphate showed deviations of clinical importance.

Conclusions: We conclude that whole blood in lithium-heparin or serum tubes stored for 10 h at 21 ± 1°C, may be used for routine analysis without restrictions for all investigated analytes but folate and phosphate.

Show MeSH
Related in: MedlinePlus