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Overexpression of a soybean ariadne-like ubiquitin ligase gene GmARI1 enhances aluminum tolerance in Arabidopsis.

Zhang X, Wang N, Chen P, Gao M, Liu J, Wang Y, Zhao T, Li Y, Gai J - PLoS ONE (2014)

Bottom Line: In vitro ubiquitination assay showed GmARI1 protein has E3 ligase activity.Overexpression of GmARI1 significantly enhanced the aluminum tolerance of transgenic Arabidopsis.These findings suggest that GmARI1 encodes a RBR type E3 ligase, which may play important roles in plant tolerance to aluminum stress.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.

ABSTRACT
Ariadne (ARI) subfamily of RBR (Ring Between Ring fingers) proteins have been found as a group of putative E3 ubiquitin ligases containing RING (Really Interesting New Gene) finger domains in fruitfly, mouse, human and Arabidopsis. Recent studies showed several RING-type E3 ubiquitin ligases play important roles in plant response to abiotic stresses, but the function of ARI in plants is largely unknown. In this study, an ariadne-like E3 ubiquitin ligase gene was isolated from soybean, Glycine max (L.) Merr., and designated as GmARI1. It encodes a predicted protein of 586 amino acids with a RBR supra-domain. Subcellular localization studies using Arabidopsis protoplast cells indicated GmARI protein was located in nucleus. The expression of GmARI1 in soybean roots was induced as early as 2-4 h after simulated stress treatments such as aluminum, which coincided with the fact of aluminum toxicity firstly and mainly acting on plant roots. In vitro ubiquitination assay showed GmARI1 protein has E3 ligase activity. Overexpression of GmARI1 significantly enhanced the aluminum tolerance of transgenic Arabidopsis. These findings suggest that GmARI1 encodes a RBR type E3 ligase, which may play important roles in plant tolerance to aluminum stress.

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Sub-cellular localizations of GmARI1 protein.A: 35S-GFP; B: 35S-GmARI1-GFP.
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pone-0111120-g005: Sub-cellular localizations of GmARI1 protein.A: 35S-GFP; B: 35S-GmARI1-GFP.

Mentions: GmARI1 gene was expressed in roots, stems, leaves, SAMs, flowers, and pods (Fig. 4). We determined the subcellular localization of the GmARI1 protein by transient expression of 35S-GmARI1-GFP in Arabidopsis protoplasts. GmARI1 was located in the nucleus, while the GFP control was mainly located in the cytoplasm (Fig. 5).


Overexpression of a soybean ariadne-like ubiquitin ligase gene GmARI1 enhances aluminum tolerance in Arabidopsis.

Zhang X, Wang N, Chen P, Gao M, Liu J, Wang Y, Zhao T, Li Y, Gai J - PLoS ONE (2014)

Sub-cellular localizations of GmARI1 protein.A: 35S-GFP; B: 35S-GmARI1-GFP.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4218711&req=5

pone-0111120-g005: Sub-cellular localizations of GmARI1 protein.A: 35S-GFP; B: 35S-GmARI1-GFP.
Mentions: GmARI1 gene was expressed in roots, stems, leaves, SAMs, flowers, and pods (Fig. 4). We determined the subcellular localization of the GmARI1 protein by transient expression of 35S-GmARI1-GFP in Arabidopsis protoplasts. GmARI1 was located in the nucleus, while the GFP control was mainly located in the cytoplasm (Fig. 5).

Bottom Line: In vitro ubiquitination assay showed GmARI1 protein has E3 ligase activity.Overexpression of GmARI1 significantly enhanced the aluminum tolerance of transgenic Arabidopsis.These findings suggest that GmARI1 encodes a RBR type E3 ligase, which may play important roles in plant tolerance to aluminum stress.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.

ABSTRACT
Ariadne (ARI) subfamily of RBR (Ring Between Ring fingers) proteins have been found as a group of putative E3 ubiquitin ligases containing RING (Really Interesting New Gene) finger domains in fruitfly, mouse, human and Arabidopsis. Recent studies showed several RING-type E3 ubiquitin ligases play important roles in plant response to abiotic stresses, but the function of ARI in plants is largely unknown. In this study, an ariadne-like E3 ubiquitin ligase gene was isolated from soybean, Glycine max (L.) Merr., and designated as GmARI1. It encodes a predicted protein of 586 amino acids with a RBR supra-domain. Subcellular localization studies using Arabidopsis protoplast cells indicated GmARI protein was located in nucleus. The expression of GmARI1 in soybean roots was induced as early as 2-4 h after simulated stress treatments such as aluminum, which coincided with the fact of aluminum toxicity firstly and mainly acting on plant roots. In vitro ubiquitination assay showed GmARI1 protein has E3 ligase activity. Overexpression of GmARI1 significantly enhanced the aluminum tolerance of transgenic Arabidopsis. These findings suggest that GmARI1 encodes a RBR type E3 ligase, which may play important roles in plant tolerance to aluminum stress.

Show MeSH
Related in: MedlinePlus