Limits...
Effects of epigallocatechin gallate on the proliferation and apoptosis of the nasopharyngeal carcinoma cell line CNE2.

Zhang W, Yang P, Gao F, Yang J, Yao K - Exp Ther Med (2014)

Bottom Line: In addition, EGCG downregulated the mRNA and protein expression of hTERT and downregulated the expression of c-Myc protein.Downregulation of the expression of hTERT and c-Myc was more evident in the high-dose group (200 μg/mL).In conclusion, EGCG has proliferation-inhibiting, cell cycle-blocking and apoptosis-promoting effects on CNE2 cells.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China ; Department of Radiotherapy, Cancer Institute and Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China.

ABSTRACT
The present study explored the effects of epigallocatechin gallate (EGCG) on the cell cycle, proliferation and apoptosis of the nasopharyngeal carcinoma cell line CNE2 in vitro. The proliferation of CNE2 cells was detected using the cell counting kit-8 method. Cell cycle distribution and apoptosis were detected using flow cytometry. The human telomerase reverse transcriptase (hTERT) mRNA expression was determined using reverse transcription polymerase chain reactions. The protein expression of hTERT and Myc proto-oncogene protein (c-Myc) was observed using western blot analysis. EGCG inhibited the proliferation of CNE2 cells in a concentration-dependent manner (P<0.05) and blocked the cell cycle progression of the cells. In the low concentration (100 μg/ml) group, the cell cycle arrest showed a time-dependent manner. However, as the concentration increased and action time was prolonged, this time dependency became less marked. EGCG promoted the apoptosis of CNE2 cells in a time-dependent manner. In addition, EGCG downregulated the mRNA and protein expression of hTERT and downregulated the expression of c-Myc protein. Downregulation of the expression of hTERT and c-Myc was more evident in the high-dose group (200 μg/mL). In conclusion, EGCG has proliferation-inhibiting, cell cycle-blocking and apoptosis-promoting effects on CNE2 cells. EGCG may be developed into an auxiliary therapeutic agent for the treatment of nasopharyngeal carcinoma.

No MeSH data available.


Related in: MedlinePlus

Effects of epigallocatechin gallate at the same concentration (100 μg/ml) on CNE2 cell cycle at different time-points, as determined using cell counting kit-8 tests. (A) Control group (0 h); (B–E) Experimental group at (B) 12 h, (C) 24 h, (D) 48 h and (E) 72 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4217784&req=5

f2-etm-08-06-1783: Effects of epigallocatechin gallate at the same concentration (100 μg/ml) on CNE2 cell cycle at different time-points, as determined using cell counting kit-8 tests. (A) Control group (0 h); (B–E) Experimental group at (B) 12 h, (C) 24 h, (D) 48 h and (E) 72 h.

Mentions: EGCG exerted a cell cycle-arresting effect on the CNE2 cells. In the low concentration (100 μg/ml) group, its blocking effect showed a time-dependent manner (Fig. 2). As the concentration increased and action time was prolonged, the effect became less evident, but the rate of apoptosis increased (Fig. 3).


Effects of epigallocatechin gallate on the proliferation and apoptosis of the nasopharyngeal carcinoma cell line CNE2.

Zhang W, Yang P, Gao F, Yang J, Yao K - Exp Ther Med (2014)

Effects of epigallocatechin gallate at the same concentration (100 μg/ml) on CNE2 cell cycle at different time-points, as determined using cell counting kit-8 tests. (A) Control group (0 h); (B–E) Experimental group at (B) 12 h, (C) 24 h, (D) 48 h and (E) 72 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4217784&req=5

f2-etm-08-06-1783: Effects of epigallocatechin gallate at the same concentration (100 μg/ml) on CNE2 cell cycle at different time-points, as determined using cell counting kit-8 tests. (A) Control group (0 h); (B–E) Experimental group at (B) 12 h, (C) 24 h, (D) 48 h and (E) 72 h.
Mentions: EGCG exerted a cell cycle-arresting effect on the CNE2 cells. In the low concentration (100 μg/ml) group, its blocking effect showed a time-dependent manner (Fig. 2). As the concentration increased and action time was prolonged, the effect became less evident, but the rate of apoptosis increased (Fig. 3).

Bottom Line: In addition, EGCG downregulated the mRNA and protein expression of hTERT and downregulated the expression of c-Myc protein.Downregulation of the expression of hTERT and c-Myc was more evident in the high-dose group (200 μg/mL).In conclusion, EGCG has proliferation-inhibiting, cell cycle-blocking and apoptosis-promoting effects on CNE2 cells.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China ; Department of Radiotherapy, Cancer Institute and Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China.

ABSTRACT
The present study explored the effects of epigallocatechin gallate (EGCG) on the cell cycle, proliferation and apoptosis of the nasopharyngeal carcinoma cell line CNE2 in vitro. The proliferation of CNE2 cells was detected using the cell counting kit-8 method. Cell cycle distribution and apoptosis were detected using flow cytometry. The human telomerase reverse transcriptase (hTERT) mRNA expression was determined using reverse transcription polymerase chain reactions. The protein expression of hTERT and Myc proto-oncogene protein (c-Myc) was observed using western blot analysis. EGCG inhibited the proliferation of CNE2 cells in a concentration-dependent manner (P<0.05) and blocked the cell cycle progression of the cells. In the low concentration (100 μg/ml) group, the cell cycle arrest showed a time-dependent manner. However, as the concentration increased and action time was prolonged, this time dependency became less marked. EGCG promoted the apoptosis of CNE2 cells in a time-dependent manner. In addition, EGCG downregulated the mRNA and protein expression of hTERT and downregulated the expression of c-Myc protein. Downregulation of the expression of hTERT and c-Myc was more evident in the high-dose group (200 μg/mL). In conclusion, EGCG has proliferation-inhibiting, cell cycle-blocking and apoptosis-promoting effects on CNE2 cells. EGCG may be developed into an auxiliary therapeutic agent for the treatment of nasopharyngeal carcinoma.

No MeSH data available.


Related in: MedlinePlus