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Mild hypothermia in combination with minimally invasive evacuation of hematoma reduces inflammatory damage in patients via the nuclear factor-κB pathway.

Bi Y, Huan Y, Cai W, Wang X, Liang Z, Liu Z, Duan R - Exp Ther Med (2014)

Bottom Line: NIHSS scores in the MHMIHE group were significantly lower than those in the MIHE group on days three and seven.TNF-α and NF-κB levels peaked on day three, and the MHMIHE group had significantly lower levels of TNF-α and NF-κB than the MIHE group.In conclusion, the present study demonstrated that mild hypothermia and minimally invasive evacuation of hematoma can effectively reduce inflammation and improve the brain function of patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China.

ABSTRACT
The aim of this study was to investigate the effects of mild hypothermia and minimally invasive evacuation of hematoma on the brain function of patients with cerebral hemorrhage. Seventy-six patients with acute cerebral hemorrhage were divided into the minimally invasive evacuation of hematoma (MIHE) and mild hypothermia and minimally invasive evacuation of hematoma (MHMIHE) groups. National Institutes of Health Stroke Scale (NIHSS) scores on the day of admission of the patient and one, three and seven days after the procedure were recorded. Perihematoma brain tissue morphology was observed using hematoxylin and eosin staining. Nuclear factor-κB (NF-κB) expression was determined by immunohistochemistry. The tumor necrosis factor-α (TNF-α) level was detected by ELISA. NIHSS scores in the MHMIHE group were significantly lower than those in the MIHE group on days three and seven. TNF-α and NF-κB levels peaked on day three, and the MHMIHE group had significantly lower levels of TNF-α and NF-κB than the MIHE group. In conclusion, the present study demonstrated that mild hypothermia and minimally invasive evacuation of hematoma can effectively reduce inflammation and improve the brain function of patients.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical assays of nuclear factor-κB expression. (A) Group MIHE, day 1 (magnification, ×400); (B) Group MHMIHE, day 1 (magnification, ×200); (C) Group MIHE, day 3 (magnification, ×400); (D) Group MHMIHE, day 3 (magnification, ×200); (E) Group MIHE, day 7 (magnification, ×400); (F) Group MHMIHE, day 7 (magnification, ×400). MIHE, minimally invasive evacuation of hematoma; MHMIHE, mild hypothermia and minimally invasive evacuation of hematoma.
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f2-etm-08-06-1717: Immunohistochemical assays of nuclear factor-κB expression. (A) Group MIHE, day 1 (magnification, ×400); (B) Group MHMIHE, day 1 (magnification, ×200); (C) Group MIHE, day 3 (magnification, ×400); (D) Group MHMIHE, day 3 (magnification, ×200); (E) Group MIHE, day 7 (magnification, ×400); (F) Group MHMIHE, day 7 (magnification, ×400). MIHE, minimally invasive evacuation of hematoma; MHMIHE, mild hypothermia and minimally invasive evacuation of hematoma.

Mentions: To assess whether mild hypothermia and minimally invasive evacuation of hematoma effectively reduced NF-κB levels in perihematoma brain tissues, immunohistochemical assays were performed. In the immunohistochemical staining (Fig. 2), NF-κB was expressed in the 76 patients with dynamic changes. NF-κB levels reached the peak on the third day and were elevated on the first and seventh days. The NF-κB expression was reduced for the MHMIHE group. The expression was mainly localized in inflammatory tissues, microglia and nerve cells. These results suggested that mild hypothermia and minimally invasive evacuation of hematoma effectively reduced the expression of NF-κB in perihematoma brain tissues and therefore alleviated inflammatory damage.


Mild hypothermia in combination with minimally invasive evacuation of hematoma reduces inflammatory damage in patients via the nuclear factor-κB pathway.

Bi Y, Huan Y, Cai W, Wang X, Liang Z, Liu Z, Duan R - Exp Ther Med (2014)

Immunohistochemical assays of nuclear factor-κB expression. (A) Group MIHE, day 1 (magnification, ×400); (B) Group MHMIHE, day 1 (magnification, ×200); (C) Group MIHE, day 3 (magnification, ×400); (D) Group MHMIHE, day 3 (magnification, ×200); (E) Group MIHE, day 7 (magnification, ×400); (F) Group MHMIHE, day 7 (magnification, ×400). MIHE, minimally invasive evacuation of hematoma; MHMIHE, mild hypothermia and minimally invasive evacuation of hematoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4217783&req=5

f2-etm-08-06-1717: Immunohistochemical assays of nuclear factor-κB expression. (A) Group MIHE, day 1 (magnification, ×400); (B) Group MHMIHE, day 1 (magnification, ×200); (C) Group MIHE, day 3 (magnification, ×400); (D) Group MHMIHE, day 3 (magnification, ×200); (E) Group MIHE, day 7 (magnification, ×400); (F) Group MHMIHE, day 7 (magnification, ×400). MIHE, minimally invasive evacuation of hematoma; MHMIHE, mild hypothermia and minimally invasive evacuation of hematoma.
Mentions: To assess whether mild hypothermia and minimally invasive evacuation of hematoma effectively reduced NF-κB levels in perihematoma brain tissues, immunohistochemical assays were performed. In the immunohistochemical staining (Fig. 2), NF-κB was expressed in the 76 patients with dynamic changes. NF-κB levels reached the peak on the third day and were elevated on the first and seventh days. The NF-κB expression was reduced for the MHMIHE group. The expression was mainly localized in inflammatory tissues, microglia and nerve cells. These results suggested that mild hypothermia and minimally invasive evacuation of hematoma effectively reduced the expression of NF-κB in perihematoma brain tissues and therefore alleviated inflammatory damage.

Bottom Line: NIHSS scores in the MHMIHE group were significantly lower than those in the MIHE group on days three and seven.TNF-α and NF-κB levels peaked on day three, and the MHMIHE group had significantly lower levels of TNF-α and NF-κB than the MIHE group.In conclusion, the present study demonstrated that mild hypothermia and minimally invasive evacuation of hematoma can effectively reduce inflammation and improve the brain function of patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China.

ABSTRACT
The aim of this study was to investigate the effects of mild hypothermia and minimally invasive evacuation of hematoma on the brain function of patients with cerebral hemorrhage. Seventy-six patients with acute cerebral hemorrhage were divided into the minimally invasive evacuation of hematoma (MIHE) and mild hypothermia and minimally invasive evacuation of hematoma (MHMIHE) groups. National Institutes of Health Stroke Scale (NIHSS) scores on the day of admission of the patient and one, three and seven days after the procedure were recorded. Perihematoma brain tissue morphology was observed using hematoxylin and eosin staining. Nuclear factor-κB (NF-κB) expression was determined by immunohistochemistry. The tumor necrosis factor-α (TNF-α) level was detected by ELISA. NIHSS scores in the MHMIHE group were significantly lower than those in the MIHE group on days three and seven. TNF-α and NF-κB levels peaked on day three, and the MHMIHE group had significantly lower levels of TNF-α and NF-κB than the MIHE group. In conclusion, the present study demonstrated that mild hypothermia and minimally invasive evacuation of hematoma can effectively reduce inflammation and improve the brain function of patients.

No MeSH data available.


Related in: MedlinePlus