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Effect of rabeprazole on the transport and distribution of levofloxacin in rat stomachs.

Bao J, Hu Y, Mei Q, Zhen H, Xu J - Exp Ther Med (2014)

Bottom Line: However, the levofloxacin concentration in the gastric body and in the antrum did not significantly differ between the two groups (P>0.05).The levofloxacin transport fractions in the stomach in the 50 mg/kg levofloxacin, 100 mg/kg levofloxacin, 50 mg/kg levofloxacin + rabeprazole and 100 mg/kg levofloxacin + rabeprazole groups were 2.36, 2.52, 2.42 and 2.55, respectively, and no significant difference was identified.Combining a proton pump inhibitor with levofloxacin has little effect on the concentration and distribution of levofloxacin in the stomach within 2 h.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, First Affiliated Hospital of Anhui Medical University, The Key Laboratory of Digestive Disease in Anhui Province, Hefei, Anhui 230022, P.R. China.

ABSTRACT
The aim of this study was to determine the transport and distribution characteristics of levofloxacin in the rat stomach and investigate the effects of combination treatment with rabeprazole. A total of 160 Wistar rats were randomly divided into four treatment groups: 50 mg/kg levofloxacin, 100 mg/kg levofloxacin, 50 mg/kg levofloxacin + rabeprazole and 100 mg/kg levofloxacin + rabeprazole. For two hours after intravenous administration, serum, gastric juice and stomach mucosa samples were collected at 15-min intervals, and the levofloxacin concentrations in all the samples were measured to determine the transport and distribution characteristics of levofloxacin in the rat stomach. In the 50 mg/kg levofloxacin and the 100 mg/kg levofloxacin groups, the drug concentration in the gastric juice gradually exceeded the serum concentration within 45-60 min of administration (P<0.05) and the drug concentrations in the gastric body and antrum were higher than those in the serum and the forestomach (P<0.05). At 15-30 min after administration, the drug concentrations in the gastric juice in the 50 mg/kg levofloxacin + rabeprazole and the 100 mg/kg levofloxacin + rabeprazole groups gradually exceeded the serum concentration (P<0.05). However, the levofloxacin concentration in the gastric body and in the antrum did not significantly differ between the two groups (P>0.05). The levofloxacin concentrations in each stomach region in the groups also treated with rabeprazole were higher than those treated with levofloxacin alone, but the differences were not significant. The levofloxacin transport fractions in the stomach in the 50 mg/kg levofloxacin, 100 mg/kg levofloxacin, 50 mg/kg levofloxacin + rabeprazole and 100 mg/kg levofloxacin + rabeprazole groups were 2.36, 2.52, 2.42 and 2.55, respectively, and no significant difference was identified. Levofloxacin may be actively transported in the rat stomach. The levofloxacin concentration in the gastric antrum exceeded that in the forestomach, and the local concentration increased with increasing dosage. Combining a proton pump inhibitor with levofloxacin has little effect on the concentration and distribution of levofloxacin in the stomach within 2 h.

No MeSH data available.


Mean concentration-time profiles of levofloxacin in the serum, gastric juice and all regions of the stomach at a 100 mg/kg dose with rabeprazole in rats.
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f3-etm-08-06-1884: Mean concentration-time profiles of levofloxacin in the serum, gastric juice and all regions of the stomach at a 100 mg/kg dose with rabeprazole in rats.

Mentions: As shown in Figs. 3 and 4, the gastric juice levofloxacin concentration was significantly higher than the serum concentration from 30 min (100 mg/kg levofloxacin + rabeprazole) and 15 min (50 mg/kg levofloxacin + rabeprazole). The levofloxacin concentration in the gastric body and gastric antrum was significantly higher than that in the serum and forestomach from 30 min (100 mg/kg levofloxacin + rabeprazole, with the exception of the 105- and 120-min time-points) and 15 min (50 mg/kg levofloxacin + rabeprazole). The levofloxacin concentration in the forestomach exhibited no significant difference from that in the serum, and no significant difference was observed in the levofloxacin concentration between the gastric body and antrum from the 15-min time-point. The stomach clearance rate of 100 mg/kg levofloxacin + rabeprazole was 1.30 l/h/kg, and the transport fraction was 2.55. The stomach clearance rate of 50 mg/kg levofloxacin + rabeprazole was 0.99 l/h/kg, and the transport fraction was 2.42.


Effect of rabeprazole on the transport and distribution of levofloxacin in rat stomachs.

Bao J, Hu Y, Mei Q, Zhen H, Xu J - Exp Ther Med (2014)

Mean concentration-time profiles of levofloxacin in the serum, gastric juice and all regions of the stomach at a 100 mg/kg dose with rabeprazole in rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4217780&req=5

f3-etm-08-06-1884: Mean concentration-time profiles of levofloxacin in the serum, gastric juice and all regions of the stomach at a 100 mg/kg dose with rabeprazole in rats.
Mentions: As shown in Figs. 3 and 4, the gastric juice levofloxacin concentration was significantly higher than the serum concentration from 30 min (100 mg/kg levofloxacin + rabeprazole) and 15 min (50 mg/kg levofloxacin + rabeprazole). The levofloxacin concentration in the gastric body and gastric antrum was significantly higher than that in the serum and forestomach from 30 min (100 mg/kg levofloxacin + rabeprazole, with the exception of the 105- and 120-min time-points) and 15 min (50 mg/kg levofloxacin + rabeprazole). The levofloxacin concentration in the forestomach exhibited no significant difference from that in the serum, and no significant difference was observed in the levofloxacin concentration between the gastric body and antrum from the 15-min time-point. The stomach clearance rate of 100 mg/kg levofloxacin + rabeprazole was 1.30 l/h/kg, and the transport fraction was 2.55. The stomach clearance rate of 50 mg/kg levofloxacin + rabeprazole was 0.99 l/h/kg, and the transport fraction was 2.42.

Bottom Line: However, the levofloxacin concentration in the gastric body and in the antrum did not significantly differ between the two groups (P>0.05).The levofloxacin transport fractions in the stomach in the 50 mg/kg levofloxacin, 100 mg/kg levofloxacin, 50 mg/kg levofloxacin + rabeprazole and 100 mg/kg levofloxacin + rabeprazole groups were 2.36, 2.52, 2.42 and 2.55, respectively, and no significant difference was identified.Combining a proton pump inhibitor with levofloxacin has little effect on the concentration and distribution of levofloxacin in the stomach within 2 h.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, First Affiliated Hospital of Anhui Medical University, The Key Laboratory of Digestive Disease in Anhui Province, Hefei, Anhui 230022, P.R. China.

ABSTRACT
The aim of this study was to determine the transport and distribution characteristics of levofloxacin in the rat stomach and investigate the effects of combination treatment with rabeprazole. A total of 160 Wistar rats were randomly divided into four treatment groups: 50 mg/kg levofloxacin, 100 mg/kg levofloxacin, 50 mg/kg levofloxacin + rabeprazole and 100 mg/kg levofloxacin + rabeprazole. For two hours after intravenous administration, serum, gastric juice and stomach mucosa samples were collected at 15-min intervals, and the levofloxacin concentrations in all the samples were measured to determine the transport and distribution characteristics of levofloxacin in the rat stomach. In the 50 mg/kg levofloxacin and the 100 mg/kg levofloxacin groups, the drug concentration in the gastric juice gradually exceeded the serum concentration within 45-60 min of administration (P<0.05) and the drug concentrations in the gastric body and antrum were higher than those in the serum and the forestomach (P<0.05). At 15-30 min after administration, the drug concentrations in the gastric juice in the 50 mg/kg levofloxacin + rabeprazole and the 100 mg/kg levofloxacin + rabeprazole groups gradually exceeded the serum concentration (P<0.05). However, the levofloxacin concentration in the gastric body and in the antrum did not significantly differ between the two groups (P>0.05). The levofloxacin concentrations in each stomach region in the groups also treated with rabeprazole were higher than those treated with levofloxacin alone, but the differences were not significant. The levofloxacin transport fractions in the stomach in the 50 mg/kg levofloxacin, 100 mg/kg levofloxacin, 50 mg/kg levofloxacin + rabeprazole and 100 mg/kg levofloxacin + rabeprazole groups were 2.36, 2.52, 2.42 and 2.55, respectively, and no significant difference was identified. Levofloxacin may be actively transported in the rat stomach. The levofloxacin concentration in the gastric antrum exceeded that in the forestomach, and the local concentration increased with increasing dosage. Combining a proton pump inhibitor with levofloxacin has little effect on the concentration and distribution of levofloxacin in the stomach within 2 h.

No MeSH data available.