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The vitamin D system is deregulated in pancreatic diseases.

Hummel D, Aggarwal A, Borka K, Bajna E, Kállay E, Horváth HC - J. Steroid Biochem. Mol. Biol. (2014)

Bottom Line: Both the VDR and the CaSR protein were highly expressed in the endocrine compared with the exocrine pancreas.In the PDAC patients CYP24A1 expression in the islets was significantly lower than in CP patients.In the transformed ducts CYP24A1 expression increases, impairing the antiproliferative effect of vitamin D in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology and Allergy Research, Medical University of Vienna, Währinger Gürtel 18-20, Leitstelle 3Q, 1090 Vienna, Austria.

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mRNA expression of the VDR and CYP24A1 is significantly increased and CaSR expression decreases in PDAC compared with adjacent non-tumorous (NT) parts of the pancreas. (A) mRNA expression (n = 11) was assessed by qRT-PCR and set relative to calibrator. (B) Data originate from a microarray analysis of mRNA from patients suffering from PDAC [28]. Median, interquartile range and whiskers according to Tukey are shown. Statistics were calculated using paired t-test on log2-transformed data. Among the values for CYP24A1 expression we identified one outlier in each dataset. (**p < 0.01, ***p < 0.001).
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fig0005: mRNA expression of the VDR and CYP24A1 is significantly increased and CaSR expression decreases in PDAC compared with adjacent non-tumorous (NT) parts of the pancreas. (A) mRNA expression (n = 11) was assessed by qRT-PCR and set relative to calibrator. (B) Data originate from a microarray analysis of mRNA from patients suffering from PDAC [28]. Median, interquartile range and whiskers according to Tukey are shown. Statistics were calculated using paired t-test on log2-transformed data. Among the values for CYP24A1 expression we identified one outlier in each dataset. (**p < 0.01, ***p < 0.001).

Mentions: In our patient cohort (Table 1), we observed a significant upregulation of VDR (mean 3.7-fold) as well as of CYP24A1 (mean 30.78-fold) mRNA expression in PDAC compared with adjacent parts of the pancreas. mRNA expression of the CaSR decreased in the tumors of most patients by 50%, however did not reach statistical significance (Fig. 1A). CYP27B1 levels did not change (data not shown). Our data are consistent with the microarray gene expression data published in the GEO database by Zhang et al. [28] (Fig. 1B), suggesting that our patient cohort was representative, despite the low sample size.


The vitamin D system is deregulated in pancreatic diseases.

Hummel D, Aggarwal A, Borka K, Bajna E, Kállay E, Horváth HC - J. Steroid Biochem. Mol. Biol. (2014)

mRNA expression of the VDR and CYP24A1 is significantly increased and CaSR expression decreases in PDAC compared with adjacent non-tumorous (NT) parts of the pancreas. (A) mRNA expression (n = 11) was assessed by qRT-PCR and set relative to calibrator. (B) Data originate from a microarray analysis of mRNA from patients suffering from PDAC [28]. Median, interquartile range and whiskers according to Tukey are shown. Statistics were calculated using paired t-test on log2-transformed data. Among the values for CYP24A1 expression we identified one outlier in each dataset. (**p < 0.01, ***p < 0.001).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4217145&req=5

fig0005: mRNA expression of the VDR and CYP24A1 is significantly increased and CaSR expression decreases in PDAC compared with adjacent non-tumorous (NT) parts of the pancreas. (A) mRNA expression (n = 11) was assessed by qRT-PCR and set relative to calibrator. (B) Data originate from a microarray analysis of mRNA from patients suffering from PDAC [28]. Median, interquartile range and whiskers according to Tukey are shown. Statistics were calculated using paired t-test on log2-transformed data. Among the values for CYP24A1 expression we identified one outlier in each dataset. (**p < 0.01, ***p < 0.001).
Mentions: In our patient cohort (Table 1), we observed a significant upregulation of VDR (mean 3.7-fold) as well as of CYP24A1 (mean 30.78-fold) mRNA expression in PDAC compared with adjacent parts of the pancreas. mRNA expression of the CaSR decreased in the tumors of most patients by 50%, however did not reach statistical significance (Fig. 1A). CYP27B1 levels did not change (data not shown). Our data are consistent with the microarray gene expression data published in the GEO database by Zhang et al. [28] (Fig. 1B), suggesting that our patient cohort was representative, despite the low sample size.

Bottom Line: Both the VDR and the CaSR protein were highly expressed in the endocrine compared with the exocrine pancreas.In the PDAC patients CYP24A1 expression in the islets was significantly lower than in CP patients.In the transformed ducts CYP24A1 expression increases, impairing the antiproliferative effect of vitamin D in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology and Allergy Research, Medical University of Vienna, Währinger Gürtel 18-20, Leitstelle 3Q, 1090 Vienna, Austria.

Show MeSH
Related in: MedlinePlus