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Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS(+) System.

Lee KJ, Song NY, Oh YC, Cho WK, Ma JY - J Anal Methods Chem (2014)

Bottom Line: Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5).The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum.The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

View Article: PubMed Central - PubMed

Affiliation: Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.

ABSTRACT
The Acer tegmentosum (3 kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (n-BuOH), and water. From the EA extract fraction (1.24 g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5). Moreover, a rapid on-line screening HPLC-ABTS(+) system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed. The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum. The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

No MeSH data available.


HPLC profile and LC-MS spectra of isolation five kind compounds from Acer tegmentosum.
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Related In: Results  -  Collection


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fig7: HPLC profile and LC-MS spectra of isolation five kind compounds from Acer tegmentosum.

Mentions: Figure 7 shows the HPLC profile and LC-MS spectra of five isolated phenolic compounds from A. tegmentosum. LC-MS analysis indicated that the compounds from A. tegmentosum were isolated in highly pure form. Each compound was dissolved in MeOH at a concentration of 200 ppm, and the LC-MS analysis conditions are listed in Table 1. LC-MS analysis is a powerful tool in metabolic profiling and metabolomics research and it can accurately determine the content of specific metabolites even at low levels in plant samples. LC-MS analysis was used previously to identify certain constituents of A. tegmentosum. The comparison of UV spectra, mass spectra, and retention times of the five types of compounds with the data of standard compounds led to their unambiguous assignments. Simultaneous analysis based on Rt of feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5) of 32.6, 34.0, 12.1, 17.7, and 21.3 min, respectively, was correlated with the molecular mass data and relative response. ESI-MS analysis of compounds feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5) showed intense [M+H]+ signals at 435.1, 435.1, 291.0, 291.0, and 453.1 m/z values, respectively.


Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS(+) System.

Lee KJ, Song NY, Oh YC, Cho WK, Ma JY - J Anal Methods Chem (2014)

HPLC profile and LC-MS spectra of isolation five kind compounds from Acer tegmentosum.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4216704&req=5

fig7: HPLC profile and LC-MS spectra of isolation five kind compounds from Acer tegmentosum.
Mentions: Figure 7 shows the HPLC profile and LC-MS spectra of five isolated phenolic compounds from A. tegmentosum. LC-MS analysis indicated that the compounds from A. tegmentosum were isolated in highly pure form. Each compound was dissolved in MeOH at a concentration of 200 ppm, and the LC-MS analysis conditions are listed in Table 1. LC-MS analysis is a powerful tool in metabolic profiling and metabolomics research and it can accurately determine the content of specific metabolites even at low levels in plant samples. LC-MS analysis was used previously to identify certain constituents of A. tegmentosum. The comparison of UV spectra, mass spectra, and retention times of the five types of compounds with the data of standard compounds led to their unambiguous assignments. Simultaneous analysis based on Rt of feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5) of 32.6, 34.0, 12.1, 17.7, and 21.3 min, respectively, was correlated with the molecular mass data and relative response. ESI-MS analysis of compounds feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5) showed intense [M+H]+ signals at 435.1, 435.1, 291.0, 291.0, and 453.1 m/z values, respectively.

Bottom Line: Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5).The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum.The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

View Article: PubMed Central - PubMed

Affiliation: Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.

ABSTRACT
The Acer tegmentosum (3 kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (n-BuOH), and water. From the EA extract fraction (1.24 g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5). Moreover, a rapid on-line screening HPLC-ABTS(+) system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed. The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum. The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

No MeSH data available.