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Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS(+) System.

Lee KJ, Song NY, Oh YC, Cho WK, Ma JY - J Anal Methods Chem (2014)

Bottom Line: Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5).The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum.The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

View Article: PubMed Central - PubMed

Affiliation: Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.

ABSTRACT
The Acer tegmentosum (3 kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (n-BuOH), and water. From the EA extract fraction (1.24 g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5). Moreover, a rapid on-line screening HPLC-ABTS(+) system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed. The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum. The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

No MeSH data available.


Process of isolation five kind compounds of ethyl acetate extract from Acer tegmentosum.
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Related In: Results  -  Collection


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fig2: Process of isolation five kind compounds of ethyl acetate extract from Acer tegmentosum.

Mentions: Dry samples (3 Kg) from the powders of the A. tegmentosum were loaded (10-times volume) in hot water extraction system. The extraction was performed by heating for 3 h at 100°C (Gyeongseo Extractor Cosmos-600, Inchon, Korea). Then, the solution was filtered using standard testing sieves (150 μm, Retsch, Haan, Germany), freeze-dried, and maintained in desiccators at 4°C prior to use. For large amount of extractions, 20 g freeze-dry samples were loaded (1 : 1, extracted thrice) and extracted successively using DCM, EA, and n-BuOH. The contents of feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5) in A. tegmentosum were remarkably higher in the EA extract (1.24 g). Then, the samples were filtered through a 0.2 μm membrane filter prior to on-line screening HPLC-ABTS+ analysis. The extraction and purification processes from A. tegmentosum are shown in Figure 2.


Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS(+) System.

Lee KJ, Song NY, Oh YC, Cho WK, Ma JY - J Anal Methods Chem (2014)

Process of isolation five kind compounds of ethyl acetate extract from Acer tegmentosum.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4216704&req=5

fig2: Process of isolation five kind compounds of ethyl acetate extract from Acer tegmentosum.
Mentions: Dry samples (3 Kg) from the powders of the A. tegmentosum were loaded (10-times volume) in hot water extraction system. The extraction was performed by heating for 3 h at 100°C (Gyeongseo Extractor Cosmos-600, Inchon, Korea). Then, the solution was filtered using standard testing sieves (150 μm, Retsch, Haan, Germany), freeze-dried, and maintained in desiccators at 4°C prior to use. For large amount of extractions, 20 g freeze-dry samples were loaded (1 : 1, extracted thrice) and extracted successively using DCM, EA, and n-BuOH. The contents of feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5) in A. tegmentosum were remarkably higher in the EA extract (1.24 g). Then, the samples were filtered through a 0.2 μm membrane filter prior to on-line screening HPLC-ABTS+ analysis. The extraction and purification processes from A. tegmentosum are shown in Figure 2.

Bottom Line: Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5).The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum.The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

View Article: PubMed Central - PubMed

Affiliation: Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.

ABSTRACT
The Acer tegmentosum (3 kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (n-BuOH), and water. From the EA extract fraction (1.24 g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. Based on spectroscopic methods such as (1)H-NMR, (13)C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), and 6'-O-galloyl salidroside (5). Moreover, a rapid on-line screening HPLC-ABTS(+) system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed. The results indicated that compounds 1 and 2 were first isolated from the A. tegmentosum. The anti-inflammatory activities and on-line screening HPLC-ABTS(+) assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A. tegmentosum.

No MeSH data available.