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The Effect of Aqueous Extract of Glycyrrhiza glabra on Herpes Simplex Virus 1.

Sabouri Ghannad M, Mohammadi A, Safiallahy S, Faradmal J, Azizi M, Ahmadvand Z - Jundishapur J Microbiol (2014)

Bottom Line: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1.The cells were incubated during various time courses.G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Molecular Medicine, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.

ABSTRACT

Background: Herpes Simplex Virus 1 (HSV-1) resistance to drugs and the side effects of drugs have drawn the attention of investigators to herbal plants.

Objectives: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1. One of the objectives of the current research was to determine the efficacy and the effect of the elapsed incubation time of treating the Vero cells infected with HSV-1 by G. glabra. In addition, the effect of cells pretreatment with licorice root extract, preincubation of virus with licorice root extract, and the antiviral activity were assessed.

Patients and methods: Vero cells were incubated after adding different concentrations of aqueous extracts of G. glabra. The cells were incubated during various time courses. Cytotoxicity assay, determining the 50% tissue culture infectious dose (TCID50), and incubation of HSV-1 with licorice root extract prior to viral infection were performed.

Results: Internal association among different experiment groups showed the significant difference in the efficacy of the extract with regard to incubation period between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours. Moreover, there was a significant difference with regard to efficacy among the pretreatment of cells with extract for two hours, incubation of virus with extract for one hour, incubation of virus with extract for two hours.

Conclusions: G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

No MeSH data available.


Related in: MedlinePlus

Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells Following Pretreatment of Virus Preparation With Licorice Root ExtractVero cells were pretreated with nontoxic concentration of extract for two hours in 37℃ with 5% CO2. In the next step, the cells were infected with HSV-1 for one-hour and two-hour incubation period. The Vero cells were washed with PBS and nontoxic concentration of the extract in DMEM was added to the monolayer cells. The microplates were monitored up to seven days for the presence of cytopathic effects. Then calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.
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fig11633: Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells Following Pretreatment of Virus Preparation With Licorice Root ExtractVero cells were pretreated with nontoxic concentration of extract for two hours in 37℃ with 5% CO2. In the next step, the cells were infected with HSV-1 for one-hour and two-hour incubation period. The Vero cells were washed with PBS and nontoxic concentration of the extract in DMEM was added to the monolayer cells. The microplates were monitored up to seven days for the presence of cytopathic effects. Then calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.

Mentions: To compare the effect of pretreatment of cells with licorice root extract and preincubation of virus with licorice root extract, new experiments were designed, ie, the results were compared to control virus. Pretreatment of cells with extract for two hours and incubation of virus with extract for one and as well as two hours had significant differences among the study groups (F = 247.412; df = 3.8; P < 0.001). Tukey’s HSD test showed the significant effect of any of the mentioned groups in comparison to the control virus (P < 0.001) (Table 3). Moreover, the comparison between pretreatment of cells with extract for two hours and incubation of virus with extract for one hour showed a significant difference in the antiviral effect of licorice root extract (P < 0.040); however such an effect was not significant in comparison to the incubation of virus with extract for two hours (P = 0.31). Incubation of virus with licorice root extract showed significant difference in antiviral effect of licorice root extract between one and two hours incubation (P < 0.001) (Table 3 and Figure 3).


The Effect of Aqueous Extract of Glycyrrhiza glabra on Herpes Simplex Virus 1.

Sabouri Ghannad M, Mohammadi A, Safiallahy S, Faradmal J, Azizi M, Ahmadvand Z - Jundishapur J Microbiol (2014)

Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells Following Pretreatment of Virus Preparation With Licorice Root ExtractVero cells were pretreated with nontoxic concentration of extract for two hours in 37℃ with 5% CO2. In the next step, the cells were infected with HSV-1 for one-hour and two-hour incubation period. The Vero cells were washed with PBS and nontoxic concentration of the extract in DMEM was added to the monolayer cells. The microplates were monitored up to seven days for the presence of cytopathic effects. Then calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216581&req=5

fig11633: Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells Following Pretreatment of Virus Preparation With Licorice Root ExtractVero cells were pretreated with nontoxic concentration of extract for two hours in 37℃ with 5% CO2. In the next step, the cells were infected with HSV-1 for one-hour and two-hour incubation period. The Vero cells were washed with PBS and nontoxic concentration of the extract in DMEM was added to the monolayer cells. The microplates were monitored up to seven days for the presence of cytopathic effects. Then calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.
Mentions: To compare the effect of pretreatment of cells with licorice root extract and preincubation of virus with licorice root extract, new experiments were designed, ie, the results were compared to control virus. Pretreatment of cells with extract for two hours and incubation of virus with extract for one and as well as two hours had significant differences among the study groups (F = 247.412; df = 3.8; P < 0.001). Tukey’s HSD test showed the significant effect of any of the mentioned groups in comparison to the control virus (P < 0.001) (Table 3). Moreover, the comparison between pretreatment of cells with extract for two hours and incubation of virus with extract for one hour showed a significant difference in the antiviral effect of licorice root extract (P < 0.040); however such an effect was not significant in comparison to the incubation of virus with extract for two hours (P = 0.31). Incubation of virus with licorice root extract showed significant difference in antiviral effect of licorice root extract between one and two hours incubation (P < 0.001) (Table 3 and Figure 3).

Bottom Line: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1.The cells were incubated during various time courses.G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Molecular Medicine, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.

ABSTRACT

Background: Herpes Simplex Virus 1 (HSV-1) resistance to drugs and the side effects of drugs have drawn the attention of investigators to herbal plants.

Objectives: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1. One of the objectives of the current research was to determine the efficacy and the effect of the elapsed incubation time of treating the Vero cells infected with HSV-1 by G. glabra. In addition, the effect of cells pretreatment with licorice root extract, preincubation of virus with licorice root extract, and the antiviral activity were assessed.

Patients and methods: Vero cells were incubated after adding different concentrations of aqueous extracts of G. glabra. The cells were incubated during various time courses. Cytotoxicity assay, determining the 50% tissue culture infectious dose (TCID50), and incubation of HSV-1 with licorice root extract prior to viral infection were performed.

Results: Internal association among different experiment groups showed the significant difference in the efficacy of the extract with regard to incubation period between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours. Moreover, there was a significant difference with regard to efficacy among the pretreatment of cells with extract for two hours, incubation of virus with extract for one hour, incubation of virus with extract for two hours.

Conclusions: G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

No MeSH data available.


Related in: MedlinePlus