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The Effect of Aqueous Extract of Glycyrrhiza glabra on Herpes Simplex Virus 1.

Sabouri Ghannad M, Mohammadi A, Safiallahy S, Faradmal J, Azizi M, Ahmadvand Z - Jundishapur J Microbiol (2014)

Bottom Line: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1.The cells were incubated during various time courses.G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Molecular Medicine, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.

ABSTRACT

Background: Herpes Simplex Virus 1 (HSV-1) resistance to drugs and the side effects of drugs have drawn the attention of investigators to herbal plants.

Objectives: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1. One of the objectives of the current research was to determine the efficacy and the effect of the elapsed incubation time of treating the Vero cells infected with HSV-1 by G. glabra. In addition, the effect of cells pretreatment with licorice root extract, preincubation of virus with licorice root extract, and the antiviral activity were assessed.

Patients and methods: Vero cells were incubated after adding different concentrations of aqueous extracts of G. glabra. The cells were incubated during various time courses. Cytotoxicity assay, determining the 50% tissue culture infectious dose (TCID50), and incubation of HSV-1 with licorice root extract prior to viral infection were performed.

Results: Internal association among different experiment groups showed the significant difference in the efficacy of the extract with regard to incubation period between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours. Moreover, there was a significant difference with regard to efficacy among the pretreatment of cells with extract for two hours, incubation of virus with extract for one hour, incubation of virus with extract for two hours.

Conclusions: G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

No MeSH data available.


Related in: MedlinePlus

Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells.After infecting the monolayer cells with HSV-1 and one-hour incubation period, nontoxic concentration of the extract in DMEM was added to the monolayer cells after one, four, eight, and twelve hours of viral infection. The 24-well plates were incubated in CO2 incubator at 37℃ and monitored daily up to seven days. Viral titer was determined by the endpoint dilution method and calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.
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fig11632: Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells.After infecting the monolayer cells with HSV-1 and one-hour incubation period, nontoxic concentration of the extract in DMEM was added to the monolayer cells after one, four, eight, and twelve hours of viral infection. The 24-well plates were incubated in CO2 incubator at 37℃ and monitored daily up to seven days. Viral titer was determined by the endpoint dilution method and calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.

Mentions: In order to assess the antiherpetic activity of the licorice root extract after virus adsorption, the licorice extract was incubated with Vero cells infected with HSV for one, four, eight, and twelve hours postinfection. The results showed that the effect of the extract was changed with regard to the elapsed time of incubation (F = 309.146; df = 4.10; P < 0.001). Tukey’s HSD test showed similar effect of the elapsed time after four and eight hours incubation of the extract in comparison to the control virus, which were not significant (P = 0.836 and P = 0.805, respectively); however, the effect of the extract was significant after one and 12 hours incubation of extract in comparison to the control virus, which was more than the logarithm variation (P < 0.001) (Table 2). Comparing the internal association among different groups of experiments showed that the different efficacy of the extract with respect to the elapsed incubation time was significant between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours (change > 1 log) (Table 2 and Figure 2).


The Effect of Aqueous Extract of Glycyrrhiza glabra on Herpes Simplex Virus 1.

Sabouri Ghannad M, Mohammadi A, Safiallahy S, Faradmal J, Azizi M, Ahmadvand Z - Jundishapur J Microbiol (2014)

Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells.After infecting the monolayer cells with HSV-1 and one-hour incubation period, nontoxic concentration of the extract in DMEM was added to the monolayer cells after one, four, eight, and twelve hours of viral infection. The 24-well plates were incubated in CO2 incubator at 37℃ and monitored daily up to seven days. Viral titer was determined by the endpoint dilution method and calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216581&req=5

fig11632: Postinfection Effect of Licorice Root Extract on HSV-1 Yield in Vero Cells.After infecting the monolayer cells with HSV-1 and one-hour incubation period, nontoxic concentration of the extract in DMEM was added to the monolayer cells after one, four, eight, and twelve hours of viral infection. The 24-well plates were incubated in CO2 incubator at 37℃ and monitored daily up to seven days. Viral titer was determined by the endpoint dilution method and calculated 50% tissue cellular infectious dose (TCID50) was compared to the control virus sample preparation.
Mentions: In order to assess the antiherpetic activity of the licorice root extract after virus adsorption, the licorice extract was incubated with Vero cells infected with HSV for one, four, eight, and twelve hours postinfection. The results showed that the effect of the extract was changed with regard to the elapsed time of incubation (F = 309.146; df = 4.10; P < 0.001). Tukey’s HSD test showed similar effect of the elapsed time after four and eight hours incubation of the extract in comparison to the control virus, which were not significant (P = 0.836 and P = 0.805, respectively); however, the effect of the extract was significant after one and 12 hours incubation of extract in comparison to the control virus, which was more than the logarithm variation (P < 0.001) (Table 2). Comparing the internal association among different groups of experiments showed that the different efficacy of the extract with respect to the elapsed incubation time was significant between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours (change > 1 log) (Table 2 and Figure 2).

Bottom Line: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1.The cells were incubated during various time courses.G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Molecular Medicine, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.

ABSTRACT

Background: Herpes Simplex Virus 1 (HSV-1) resistance to drugs and the side effects of drugs have drawn the attention of investigators to herbal plants.

Objectives: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1. One of the objectives of the current research was to determine the efficacy and the effect of the elapsed incubation time of treating the Vero cells infected with HSV-1 by G. glabra. In addition, the effect of cells pretreatment with licorice root extract, preincubation of virus with licorice root extract, and the antiviral activity were assessed.

Patients and methods: Vero cells were incubated after adding different concentrations of aqueous extracts of G. glabra. The cells were incubated during various time courses. Cytotoxicity assay, determining the 50% tissue culture infectious dose (TCID50), and incubation of HSV-1 with licorice root extract prior to viral infection were performed.

Results: Internal association among different experiment groups showed the significant difference in the efficacy of the extract with regard to incubation period between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours. Moreover, there was a significant difference with regard to efficacy among the pretreatment of cells with extract for two hours, incubation of virus with extract for one hour, incubation of virus with extract for two hours.

Conclusions: G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

No MeSH data available.


Related in: MedlinePlus