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The Effect of Aqueous Extract of Glycyrrhiza glabra on Herpes Simplex Virus 1.

Sabouri Ghannad M, Mohammadi A, Safiallahy S, Faradmal J, Azizi M, Ahmadvand Z - Jundishapur J Microbiol (2014)

Bottom Line: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1.The cells were incubated during various time courses.G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Molecular Medicine, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.

ABSTRACT

Background: Herpes Simplex Virus 1 (HSV-1) resistance to drugs and the side effects of drugs have drawn the attention of investigators to herbal plants.

Objectives: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1. One of the objectives of the current research was to determine the efficacy and the effect of the elapsed incubation time of treating the Vero cells infected with HSV-1 by G. glabra. In addition, the effect of cells pretreatment with licorice root extract, preincubation of virus with licorice root extract, and the antiviral activity were assessed.

Patients and methods: Vero cells were incubated after adding different concentrations of aqueous extracts of G. glabra. The cells were incubated during various time courses. Cytotoxicity assay, determining the 50% tissue culture infectious dose (TCID50), and incubation of HSV-1 with licorice root extract prior to viral infection were performed.

Results: Internal association among different experiment groups showed the significant difference in the efficacy of the extract with regard to incubation period between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours. Moreover, there was a significant difference with regard to efficacy among the pretreatment of cells with extract for two hours, incubation of virus with extract for one hour, incubation of virus with extract for two hours.

Conclusions: G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

No MeSH data available.


Related in: MedlinePlus

Staining the Vero Cells.A, Vero cells before staining with Neutral Red dye. B, Vero cells after treating with G. glabra and staining with Neutral Red dye.
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fig11631: Staining the Vero Cells.A, Vero cells before staining with Neutral Red dye. B, Vero cells after treating with G. glabra and staining with Neutral Red dye.

Mentions: In order to determine the appropriate concentration of aqueous extract of licorice root, which has less than 50% cytotoxicity for Vero cells, the Neutral Red assay was employed. Microtitre plates containing 96-well tissue culture plates were inoculated with 105 Vero cells to achieve the confluence of 80%. The cells were washed with pre-warmed phosphate buffered saline (PBS). Then different concentrations of licorice root extract prepared in DMEM were added to the assigned wells. The plates were incubated at 37℃ with 5% CO2 for two days. After two days, the Vero cells were washed by pre-warmed PBS and filtered Neutral Red solution was added to each well (The viable cells absorb the Neutral Red dye). The plates were incubated in CO2 incubator at 37℃ for three hours. After incubation, the cells were washed by pre-warmed PBS and Neutral Red dye stain solution was added to each well. The plates were shaken in dark on the shaker for ten minutes until the Neutral Red dye was removed. The optical density of the Neutral Red solution was measured at 540 nm by ELISA reader (Sunrise Remote, Tecan, Austria) (Table 1). The highest concentration that had less than 50% cytotoxicity for Vero cells (0.2 mg/mL) was used for further experiments (Figure 1).


The Effect of Aqueous Extract of Glycyrrhiza glabra on Herpes Simplex Virus 1.

Sabouri Ghannad M, Mohammadi A, Safiallahy S, Faradmal J, Azizi M, Ahmadvand Z - Jundishapur J Microbiol (2014)

Staining the Vero Cells.A, Vero cells before staining with Neutral Red dye. B, Vero cells after treating with G. glabra and staining with Neutral Red dye.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216581&req=5

fig11631: Staining the Vero Cells.A, Vero cells before staining with Neutral Red dye. B, Vero cells after treating with G. glabra and staining with Neutral Red dye.
Mentions: In order to determine the appropriate concentration of aqueous extract of licorice root, which has less than 50% cytotoxicity for Vero cells, the Neutral Red assay was employed. Microtitre plates containing 96-well tissue culture plates were inoculated with 105 Vero cells to achieve the confluence of 80%. The cells were washed with pre-warmed phosphate buffered saline (PBS). Then different concentrations of licorice root extract prepared in DMEM were added to the assigned wells. The plates were incubated at 37℃ with 5% CO2 for two days. After two days, the Vero cells were washed by pre-warmed PBS and filtered Neutral Red solution was added to each well (The viable cells absorb the Neutral Red dye). The plates were incubated in CO2 incubator at 37℃ for three hours. After incubation, the cells were washed by pre-warmed PBS and Neutral Red dye stain solution was added to each well. The plates were shaken in dark on the shaker for ten minutes until the Neutral Red dye was removed. The optical density of the Neutral Red solution was measured at 540 nm by ELISA reader (Sunrise Remote, Tecan, Austria) (Table 1). The highest concentration that had less than 50% cytotoxicity for Vero cells (0.2 mg/mL) was used for further experiments (Figure 1).

Bottom Line: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1.The cells were incubated during various time courses.G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Molecular Medicine, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.

ABSTRACT

Background: Herpes Simplex Virus 1 (HSV-1) resistance to drugs and the side effects of drugs have drawn the attention of investigators to herbal plants.

Objectives: The main aim of the current research was to investigate the effects of Glycyrrhiza glabra (liquorice root) on HSV-1. One of the objectives of the current research was to determine the efficacy and the effect of the elapsed incubation time of treating the Vero cells infected with HSV-1 by G. glabra. In addition, the effect of cells pretreatment with licorice root extract, preincubation of virus with licorice root extract, and the antiviral activity were assessed.

Patients and methods: Vero cells were incubated after adding different concentrations of aqueous extracts of G. glabra. The cells were incubated during various time courses. Cytotoxicity assay, determining the 50% tissue culture infectious dose (TCID50), and incubation of HSV-1 with licorice root extract prior to viral infection were performed.

Results: Internal association among different experiment groups showed the significant difference in the efficacy of the extract with regard to incubation period between one and four hours, one and eight hours, four and 12 hours, and eight and 12 hours. Moreover, there was a significant difference with regard to efficacy among the pretreatment of cells with extract for two hours, incubation of virus with extract for one hour, incubation of virus with extract for two hours.

Conclusions: G. glabra showed the characteristics of a novel antiviral medication; however, more in vitro experiments are needed to determine the antiherpetic activities of the G. glabra.

No MeSH data available.


Related in: MedlinePlus