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Effects of sera taken from women with recurrent spontaneous abortion on sperm motility and apoptosis.

Talaei-Khozani T, Borzoei Z, Bahmanpour S, Zolghadr J, Dehbashi S, Zareh HR - Iran J Reprod Med (2011)

Bottom Line: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation.However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Background: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities.

Objective: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.

Materials and methods: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test.

Results: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant.

Conclusion: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.

No MeSH data available.


Related in: MedlinePlus

Comparison of the TUNEL positive sperms after exposure to the sera. Negative control (A), Dot plot (B), one sample that was exposed to sera from woman with RSA with 33.18% of TUNEL positive cells (C), one sample that was exposed to the sera from normal woman with 4.74% of TUNEL positive cells (D).
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Figure 2: Comparison of the TUNEL positive sperms after exposure to the sera. Negative control (A), Dot plot (B), one sample that was exposed to sera from woman with RSA with 33.18% of TUNEL positive cells (C), one sample that was exposed to the sera from normal woman with 4.74% of TUNEL positive cells (D).

Mentions: The TUNEL positive sperm head with green fluorescence is shown in figure 1. Flow cytometery assay showed the median value of the percentage of the TUNEL positive sperms was reduced after exposure to the sera from women with RSA; however, it was not statistically significant (p=0.722). In the semen samples exposed to sera taken from RSA patients, the median value of the percentage of TUNEL positive spermatozoa was 26.6%. The median value of the percentage of TUNEL-positive spermatozoa was 21.2% in control samples. Figure 2 demonstrates the histogram of the TUNEL assay analyzed by flow cytometry. The experimental semen contained less TUNEL positive cells that indicated sperms with DNA fragmentation nucleus.


Effects of sera taken from women with recurrent spontaneous abortion on sperm motility and apoptosis.

Talaei-Khozani T, Borzoei Z, Bahmanpour S, Zolghadr J, Dehbashi S, Zareh HR - Iran J Reprod Med (2011)

Comparison of the TUNEL positive sperms after exposure to the sera. Negative control (A), Dot plot (B), one sample that was exposed to sera from woman with RSA with 33.18% of TUNEL positive cells (C), one sample that was exposed to the sera from normal woman with 4.74% of TUNEL positive cells (D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216447&req=5

Figure 2: Comparison of the TUNEL positive sperms after exposure to the sera. Negative control (A), Dot plot (B), one sample that was exposed to sera from woman with RSA with 33.18% of TUNEL positive cells (C), one sample that was exposed to the sera from normal woman with 4.74% of TUNEL positive cells (D).
Mentions: The TUNEL positive sperm head with green fluorescence is shown in figure 1. Flow cytometery assay showed the median value of the percentage of the TUNEL positive sperms was reduced after exposure to the sera from women with RSA; however, it was not statistically significant (p=0.722). In the semen samples exposed to sera taken from RSA patients, the median value of the percentage of TUNEL positive spermatozoa was 26.6%. The median value of the percentage of TUNEL-positive spermatozoa was 21.2% in control samples. Figure 2 demonstrates the histogram of the TUNEL assay analyzed by flow cytometry. The experimental semen contained less TUNEL positive cells that indicated sperms with DNA fragmentation nucleus.

Bottom Line: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation.However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Background: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities.

Objective: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.

Materials and methods: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test.

Results: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant.

Conclusion: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.

No MeSH data available.


Related in: MedlinePlus