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Tomographic and histometric analysis of autogenous bone block and synthetic hydroxyapatite block grafts without rigid fixation on rabbit calvaria.

Bae SY, Park JC, Shin HS, Lee YK, Choi SH, Jung UW - J Periodontal Implant Sci (2014)

Bottom Line: The animals were sacrificed at 4 or 8 weeks postoperatively, and the grafted materials were analyzed at each healing period using microcomputed tomography and histologic evaluation.Integration of the graft and the recipient bed was observed in all specimens, although minor dislocation of the graft materials from the original position was evident in some specimens (six ABB and ten HAB samples).The nonfixed HAB was successfully integrated into the recipient bed after both healing periods in the rabbit calvaria.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: The preferred material for bone augmentation beyond the envelope of skeletal bone is the bone block graft, due to its dimensional stability. We evaluated the necessity of rigid fixation for the bone block graft, and compared the bone regeneration and volume maintenance associated with grafting using a synthetic hydroxyapatite block (HAB) and an autogenous bone block (ABB) without rigid fixation on rabbit calvaria over two different periods.

Methods: Cylinder-shaped synthetic HAB and ABB were positioned without fixation on the rabbit calvarium (n=16). The animals were sacrificed at 4 or 8 weeks postoperatively, and the grafted materials were analyzed at each healing period using microcomputed tomography and histologic evaluation.

Results: Integration of the graft and the recipient bed was observed in all specimens, although minor dislocation of the graft materials from the original position was evident in some specimens (six ABB and ten HAB samples). A tendency toward progressive bone resorption was observed in the grafted ABB but not in the grafted HAB, which maintained an intact appearance. In the HAB group, the area of new bone increased between 4 and 8 weeks postoperatively, but the difference was not statistically significant.

Conclusions: The nonfixed HAB was successfully integrated into the recipient bed after both healing periods in the rabbit calvaria. In spite of limited bone formation activity in comparison to ABB, HAB may be a favorable substitute osteoconductive bone material.

No MeSH data available.


Related in: MedlinePlus

Histologic views of the hydroxyapatite block (HAB) grafted area after 4 weeks (H&E). (A) Perimeter of grafted material over the residual bone. Residual materials are observed within the perimeter of the grafted HAB. (B) A moderate amount of new bone growth into the HAB was observed over the residual bone. (C) Highly lamellated new bone growth is observed among the HAB graft. Inset boxes at lower magnification (×40) represent the corresponding area at higher magnification (×200) and corresponding figures are labelled accordingly. RB: residual bone, NB: new bone, HA: remaining hydroxyapatite.
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Figure 5: Histologic views of the hydroxyapatite block (HAB) grafted area after 4 weeks (H&E). (A) Perimeter of grafted material over the residual bone. Residual materials are observed within the perimeter of the grafted HAB. (B) A moderate amount of new bone growth into the HAB was observed over the residual bone. (C) Highly lamellated new bone growth is observed among the HAB graft. Inset boxes at lower magnification (×40) represent the corresponding area at higher magnification (×200) and corresponding figures are labelled accordingly. RB: residual bone, NB: new bone, HA: remaining hydroxyapatite.

Mentions: At 4 and 8 weeks, the grafted HAB materials were well maintained under the connective tissue layer, and there was little or no change in the perimeter at either point in time. There was no sign of severe inflammation or exposure of the material. Intimate contact between graft materials and the recipient bed at the interface surface-indicating bony union-was observed in both groups. The HAB appeared to be infiltrated by newly formed bone, without signs of necrosis or osteolysis, and direct contact was observed between the newly formed bone and the graft materials, especially at 8 weeks. Newly formed bone was observed along the interface between the recipient bone and the block, and extensive vascular tissue was observed within the remaining graft materials. Resorption of graft materials was barely noticeable over the healing period (Figs. 5 and 6).


Tomographic and histometric analysis of autogenous bone block and synthetic hydroxyapatite block grafts without rigid fixation on rabbit calvaria.

Bae SY, Park JC, Shin HS, Lee YK, Choi SH, Jung UW - J Periodontal Implant Sci (2014)

Histologic views of the hydroxyapatite block (HAB) grafted area after 4 weeks (H&E). (A) Perimeter of grafted material over the residual bone. Residual materials are observed within the perimeter of the grafted HAB. (B) A moderate amount of new bone growth into the HAB was observed over the residual bone. (C) Highly lamellated new bone growth is observed among the HAB graft. Inset boxes at lower magnification (×40) represent the corresponding area at higher magnification (×200) and corresponding figures are labelled accordingly. RB: residual bone, NB: new bone, HA: remaining hydroxyapatite.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216402&req=5

Figure 5: Histologic views of the hydroxyapatite block (HAB) grafted area after 4 weeks (H&E). (A) Perimeter of grafted material over the residual bone. Residual materials are observed within the perimeter of the grafted HAB. (B) A moderate amount of new bone growth into the HAB was observed over the residual bone. (C) Highly lamellated new bone growth is observed among the HAB graft. Inset boxes at lower magnification (×40) represent the corresponding area at higher magnification (×200) and corresponding figures are labelled accordingly. RB: residual bone, NB: new bone, HA: remaining hydroxyapatite.
Mentions: At 4 and 8 weeks, the grafted HAB materials were well maintained under the connective tissue layer, and there was little or no change in the perimeter at either point in time. There was no sign of severe inflammation or exposure of the material. Intimate contact between graft materials and the recipient bed at the interface surface-indicating bony union-was observed in both groups. The HAB appeared to be infiltrated by newly formed bone, without signs of necrosis or osteolysis, and direct contact was observed between the newly formed bone and the graft materials, especially at 8 weeks. Newly formed bone was observed along the interface between the recipient bone and the block, and extensive vascular tissue was observed within the remaining graft materials. Resorption of graft materials was barely noticeable over the healing period (Figs. 5 and 6).

Bottom Line: The animals were sacrificed at 4 or 8 weeks postoperatively, and the grafted materials were analyzed at each healing period using microcomputed tomography and histologic evaluation.Integration of the graft and the recipient bed was observed in all specimens, although minor dislocation of the graft materials from the original position was evident in some specimens (six ABB and ten HAB samples).The nonfixed HAB was successfully integrated into the recipient bed after both healing periods in the rabbit calvaria.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: The preferred material for bone augmentation beyond the envelope of skeletal bone is the bone block graft, due to its dimensional stability. We evaluated the necessity of rigid fixation for the bone block graft, and compared the bone regeneration and volume maintenance associated with grafting using a synthetic hydroxyapatite block (HAB) and an autogenous bone block (ABB) without rigid fixation on rabbit calvaria over two different periods.

Methods: Cylinder-shaped synthetic HAB and ABB were positioned without fixation on the rabbit calvarium (n=16). The animals were sacrificed at 4 or 8 weeks postoperatively, and the grafted materials were analyzed at each healing period using microcomputed tomography and histologic evaluation.

Results: Integration of the graft and the recipient bed was observed in all specimens, although minor dislocation of the graft materials from the original position was evident in some specimens (six ABB and ten HAB samples). A tendency toward progressive bone resorption was observed in the grafted ABB but not in the grafted HAB, which maintained an intact appearance. In the HAB group, the area of new bone increased between 4 and 8 weeks postoperatively, but the difference was not statistically significant.

Conclusions: The nonfixed HAB was successfully integrated into the recipient bed after both healing periods in the rabbit calvaria. In spite of limited bone formation activity in comparison to ABB, HAB may be a favorable substitute osteoconductive bone material.

No MeSH data available.


Related in: MedlinePlus