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Early bone healing onto implant surface treated by fibronectin/oxysterol for cell adhesion/osteogenic differentiation: in vivo experimental study in dogs.

Lee JS, Yang JH, Hong JY, Jung UW, Yang HC, Lee IS, Choi SH - J Periodontal Implant Sci (2014)

Bottom Line: A randomly selected unilateral ridge was observed for 2 weeks, and the contralateral ridge for a 4-week period.Histometric results revealed a significant reduction in the BIC in MI, AMI, and OAMI compared to SLAI, but FAMI demonstrated a comparable BIC with SLAI.Although both the BIC and bone density increased from a 2- to 4-week healing period, bone density showed no significant difference among any of the experimental and control groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: This study aimed to evaluate the effects of fibronectin and oxysterol immobilized on machined-surface dental implants for the enhancement of cell attachment and osteogenic differentiation, on peri-implant bone healing in the early healing phase using an experimental model in dogs.

Methods: Five types of dental implants were installed at a healed alveolar ridge in five dogs: a machined-surface implant (MI), apatite-coated MI (AMI), fibronectin-loaded AMI (FAMI), oxysterol-loaded AMI (OAMI), and sand-blasted, large-grit, acid-etched surface implant (SLAI). A randomly selected unilateral ridge was observed for 2 weeks, and the contralateral ridge for a 4-week period. Histologic and histometric analyses were performed for the bone-to-implant contact proportion (BIC) and bone density around the dental implant surface.

Results: Different bone healing patterns were observed according to the type of implant surface 2 weeks after installation; newly formed bone continuously lined the entire surfaces in specimens of the FAMI and SLAI groups, whereas bony trabecula from adjacent bone tissue appeared with minimal new bone lining onto the surface in the MI, AMI, and OAMI groups. Histometric results revealed a significant reduction in the BIC in MI, AMI, and OAMI compared to SLAI, but FAMI demonstrated a comparable BIC with SLAI. Although both the BIC and bone density increased from a 2- to 4-week healing period, bone density showed no significant difference among any of the experimental and control groups.

Conclusions: A fibronectin-coated implant surface designed for cell adhesion could increase contact osteogenesis in the early bone healing phase, but an oxysterol-coated implant surface designed for osteoinductivity could not modify early bone healing around implants in normal bone physiology.

No MeSH data available.


Related in: MedlinePlus

Representative photomicrographs of all experimental and control groups at 4 weeks after implant installation. The woven bone area had decreased in all of the specimens at the 4-week observational period. Most of the spaces between threads were filled with lamellated bone rather than woven bone, and increased, direct contact of bone to dental implants could be found along the whole length of the dental implants. There were still no significant differences in bone healing visible at the bone-to-implant interface in low magnification views. (A) Machined-surface implant (MI), (B) apatite-coated MI (AMI), (C) fibronectin-loaded and AMI, (D) oxysterol-loaded and AMI, and (E) sand-blasted, large-grit, and acid-etched surface implant. The scale bars in all panels represented 1 mm.
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Figure 3: Representative photomicrographs of all experimental and control groups at 4 weeks after implant installation. The woven bone area had decreased in all of the specimens at the 4-week observational period. Most of the spaces between threads were filled with lamellated bone rather than woven bone, and increased, direct contact of bone to dental implants could be found along the whole length of the dental implants. There were still no significant differences in bone healing visible at the bone-to-implant interface in low magnification views. (A) Machined-surface implant (MI), (B) apatite-coated MI (AMI), (C) fibronectin-loaded and AMI, (D) oxysterol-loaded and AMI, and (E) sand-blasted, large-grit, and acid-etched surface implant. The scale bars in all panels represented 1 mm.

Mentions: In the specimens from all groups at 4 weeks, newly formed bone tissue increased around the surface and in the space between the implant threads (Figs. 3 and 4). The proportion of newly formed woven bone around the implant was reduced at 4 weeks compared to 2 weeks.


Early bone healing onto implant surface treated by fibronectin/oxysterol for cell adhesion/osteogenic differentiation: in vivo experimental study in dogs.

Lee JS, Yang JH, Hong JY, Jung UW, Yang HC, Lee IS, Choi SH - J Periodontal Implant Sci (2014)

Representative photomicrographs of all experimental and control groups at 4 weeks after implant installation. The woven bone area had decreased in all of the specimens at the 4-week observational period. Most of the spaces between threads were filled with lamellated bone rather than woven bone, and increased, direct contact of bone to dental implants could be found along the whole length of the dental implants. There were still no significant differences in bone healing visible at the bone-to-implant interface in low magnification views. (A) Machined-surface implant (MI), (B) apatite-coated MI (AMI), (C) fibronectin-loaded and AMI, (D) oxysterol-loaded and AMI, and (E) sand-blasted, large-grit, and acid-etched surface implant. The scale bars in all panels represented 1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216401&req=5

Figure 3: Representative photomicrographs of all experimental and control groups at 4 weeks after implant installation. The woven bone area had decreased in all of the specimens at the 4-week observational period. Most of the spaces between threads were filled with lamellated bone rather than woven bone, and increased, direct contact of bone to dental implants could be found along the whole length of the dental implants. There were still no significant differences in bone healing visible at the bone-to-implant interface in low magnification views. (A) Machined-surface implant (MI), (B) apatite-coated MI (AMI), (C) fibronectin-loaded and AMI, (D) oxysterol-loaded and AMI, and (E) sand-blasted, large-grit, and acid-etched surface implant. The scale bars in all panels represented 1 mm.
Mentions: In the specimens from all groups at 4 weeks, newly formed bone tissue increased around the surface and in the space between the implant threads (Figs. 3 and 4). The proportion of newly formed woven bone around the implant was reduced at 4 weeks compared to 2 weeks.

Bottom Line: A randomly selected unilateral ridge was observed for 2 weeks, and the contralateral ridge for a 4-week period.Histometric results revealed a significant reduction in the BIC in MI, AMI, and OAMI compared to SLAI, but FAMI demonstrated a comparable BIC with SLAI.Although both the BIC and bone density increased from a 2- to 4-week healing period, bone density showed no significant difference among any of the experimental and control groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: This study aimed to evaluate the effects of fibronectin and oxysterol immobilized on machined-surface dental implants for the enhancement of cell attachment and osteogenic differentiation, on peri-implant bone healing in the early healing phase using an experimental model in dogs.

Methods: Five types of dental implants were installed at a healed alveolar ridge in five dogs: a machined-surface implant (MI), apatite-coated MI (AMI), fibronectin-loaded AMI (FAMI), oxysterol-loaded AMI (OAMI), and sand-blasted, large-grit, acid-etched surface implant (SLAI). A randomly selected unilateral ridge was observed for 2 weeks, and the contralateral ridge for a 4-week period. Histologic and histometric analyses were performed for the bone-to-implant contact proportion (BIC) and bone density around the dental implant surface.

Results: Different bone healing patterns were observed according to the type of implant surface 2 weeks after installation; newly formed bone continuously lined the entire surfaces in specimens of the FAMI and SLAI groups, whereas bony trabecula from adjacent bone tissue appeared with minimal new bone lining onto the surface in the MI, AMI, and OAMI groups. Histometric results revealed a significant reduction in the BIC in MI, AMI, and OAMI compared to SLAI, but FAMI demonstrated a comparable BIC with SLAI. Although both the BIC and bone density increased from a 2- to 4-week healing period, bone density showed no significant difference among any of the experimental and control groups.

Conclusions: A fibronectin-coated implant surface designed for cell adhesion could increase contact osteogenesis in the early bone healing phase, but an oxysterol-coated implant surface designed for osteoinductivity could not modify early bone healing around implants in normal bone physiology.

No MeSH data available.


Related in: MedlinePlus