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Overexpression of two PsnAP1 genes from Populus simonii × P. nigra causes early flowering in transgenic tobacco and Arabidopsis.

Zheng T, Li S, Zang L, Dai L, Yang C, Qu GZ - PLoS ONE (2014)

Bottom Line: Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering.These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11.Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China.

ABSTRACT
In Arabidopsis, AP1 is a floral meristem identity gene and plays an important role in floral organ development. In this study, PsnAP1-1 and PsnAP1-2 were isolated from the male reproductive buds of poplar (Populus simonii × P. nigra), which are the orthologs of AP1 in Arabidopsis, by sequence analysis. Northern blot and qRT-PCR analysis showed that PsnAP1-1 and PsnAP1-2 exhibited high expression level in early inflorescence development of poplar. Subcellular localization showed the PsnAP1-1 and PsnAP1-2 proteins are localized in the nucleus. Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering. These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11. Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent. This study indicates that PsnAP1-1 and PsnAP1-2 play a role in floral transition of poplar.

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Relative expression levels of flowering-related genes in tobacco.The 35S::PsnAP1-1 and 35S::PsnAP1-2 plants showed significant differences in relative expression level of flowering-related genes compared with wild-type using student's t-test, (P≤0.01) by SPSS 11.5 analysis. Values are expressed as mean (n = 3); error bars denote SD. MADS3 NsMADS3 (No. AF068722), MADS4 NtMADS4 (No. AF068723), MADS5 NtMADS5 (No. AF068724), MADS11 NtMADS11 (No. AF385746), SOC1 NtSOC1 (No. X76188), FUL NtFUL (No. DQ534202) were induced in transgenic plant.
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pone-0111725-g009: Relative expression levels of flowering-related genes in tobacco.The 35S::PsnAP1-1 and 35S::PsnAP1-2 plants showed significant differences in relative expression level of flowering-related genes compared with wild-type using student's t-test, (P≤0.01) by SPSS 11.5 analysis. Values are expressed as mean (n = 3); error bars denote SD. MADS3 NsMADS3 (No. AF068722), MADS4 NtMADS4 (No. AF068723), MADS5 NtMADS5 (No. AF068724), MADS11 NtMADS11 (No. AF385746), SOC1 NtSOC1 (No. X76188), FUL NtFUL (No. DQ534202) were induced in transgenic plant.

Mentions: qRT-PCR was performed to estimate the transcription levels of flowering-related genes using the total RNA from aerial parts of 25-day-old transgenic and wild-type tobacco plants, because overexpression of some MADS-box genes was found to induce early flowering and enhance expression of endogenous flowering-related genes in tobacco [22], [23]. Several genes reported to be related to flowering, such as NsMADS3 (GenBank Accession No. AF068722), NtMADS4 (No. AF068723), NtMADS5 (No. AF068724), NtMADS11 (No. AF385746), NtSOC1 (No. X76188), and NtFUL (No. DQ534202) were selected for analysis. As shown in Fig. 9, there was significantly higher expression of all these genes in transgenic tobacco than in wild type. Specifically, the expression of NsMADS3, NtMADS4, NtMADS5, and NtMADS11 was more than 20-fold higher in transgenic tobacco than in wild-type plants. The expression of NtSOC1 and NtFUL was more than 10-fold higher in 35S::PsnAP1-2 tobacco than in wild-type plants.


Overexpression of two PsnAP1 genes from Populus simonii × P. nigra causes early flowering in transgenic tobacco and Arabidopsis.

Zheng T, Li S, Zang L, Dai L, Yang C, Qu GZ - PLoS ONE (2014)

Relative expression levels of flowering-related genes in tobacco.The 35S::PsnAP1-1 and 35S::PsnAP1-2 plants showed significant differences in relative expression level of flowering-related genes compared with wild-type using student's t-test, (P≤0.01) by SPSS 11.5 analysis. Values are expressed as mean (n = 3); error bars denote SD. MADS3 NsMADS3 (No. AF068722), MADS4 NtMADS4 (No. AF068723), MADS5 NtMADS5 (No. AF068724), MADS11 NtMADS11 (No. AF385746), SOC1 NtSOC1 (No. X76188), FUL NtFUL (No. DQ534202) were induced in transgenic plant.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4216142&req=5

pone-0111725-g009: Relative expression levels of flowering-related genes in tobacco.The 35S::PsnAP1-1 and 35S::PsnAP1-2 plants showed significant differences in relative expression level of flowering-related genes compared with wild-type using student's t-test, (P≤0.01) by SPSS 11.5 analysis. Values are expressed as mean (n = 3); error bars denote SD. MADS3 NsMADS3 (No. AF068722), MADS4 NtMADS4 (No. AF068723), MADS5 NtMADS5 (No. AF068724), MADS11 NtMADS11 (No. AF385746), SOC1 NtSOC1 (No. X76188), FUL NtFUL (No. DQ534202) were induced in transgenic plant.
Mentions: qRT-PCR was performed to estimate the transcription levels of flowering-related genes using the total RNA from aerial parts of 25-day-old transgenic and wild-type tobacco plants, because overexpression of some MADS-box genes was found to induce early flowering and enhance expression of endogenous flowering-related genes in tobacco [22], [23]. Several genes reported to be related to flowering, such as NsMADS3 (GenBank Accession No. AF068722), NtMADS4 (No. AF068723), NtMADS5 (No. AF068724), NtMADS11 (No. AF385746), NtSOC1 (No. X76188), and NtFUL (No. DQ534202) were selected for analysis. As shown in Fig. 9, there was significantly higher expression of all these genes in transgenic tobacco than in wild type. Specifically, the expression of NsMADS3, NtMADS4, NtMADS5, and NtMADS11 was more than 20-fold higher in transgenic tobacco than in wild-type plants. The expression of NtSOC1 and NtFUL was more than 10-fold higher in 35S::PsnAP1-2 tobacco than in wild-type plants.

Bottom Line: Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering.These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11.Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China.

ABSTRACT
In Arabidopsis, AP1 is a floral meristem identity gene and plays an important role in floral organ development. In this study, PsnAP1-1 and PsnAP1-2 were isolated from the male reproductive buds of poplar (Populus simonii × P. nigra), which are the orthologs of AP1 in Arabidopsis, by sequence analysis. Northern blot and qRT-PCR analysis showed that PsnAP1-1 and PsnAP1-2 exhibited high expression level in early inflorescence development of poplar. Subcellular localization showed the PsnAP1-1 and PsnAP1-2 proteins are localized in the nucleus. Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering. These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11. Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent. This study indicates that PsnAP1-1 and PsnAP1-2 play a role in floral transition of poplar.

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