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Overexpression of two PsnAP1 genes from Populus simonii × P. nigra causes early flowering in transgenic tobacco and Arabidopsis.

Zheng T, Li S, Zang L, Dai L, Yang C, Qu GZ - PLoS ONE (2014)

Bottom Line: Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering.These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11.Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China.

ABSTRACT
In Arabidopsis, AP1 is a floral meristem identity gene and plays an important role in floral organ development. In this study, PsnAP1-1 and PsnAP1-2 were isolated from the male reproductive buds of poplar (Populus simonii × P. nigra), which are the orthologs of AP1 in Arabidopsis, by sequence analysis. Northern blot and qRT-PCR analysis showed that PsnAP1-1 and PsnAP1-2 exhibited high expression level in early inflorescence development of poplar. Subcellular localization showed the PsnAP1-1 and PsnAP1-2 proteins are localized in the nucleus. Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering. These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11. Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent. This study indicates that PsnAP1-1 and PsnAP1-2 play a role in floral transition of poplar.

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Chlorophyll content and photosynthesis.(A) Chlorophyll content. The leaves were collect from 30-day-old tobacco plant. Each transgenic line showed significant differences compared with wild-type by SPSS 11.5 analysis (Student's t-test, P≤0.01). Values are expressed as means (n = 3 leaves for each test), error bars denote SD. ** P≤0.01 for t test. WT wild-type, 35S::PsnAP1-1 and 35S::PsnAP1-2 the different transgenic lines. Chla chlorophyll a, Chlb chlorophyll b, Car carotenoid. B Starch-iodine staining analysis. After 3 h culture under normal light condition, the leaves were removed for starch-iodine staining analysis. WT wild-type, PsnAP1-1 35S::PsnAP1-1 tobacco, PsnAP1-2 35S::PsnAP1-2 tobacco.
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pone-0111725-g007: Chlorophyll content and photosynthesis.(A) Chlorophyll content. The leaves were collect from 30-day-old tobacco plant. Each transgenic line showed significant differences compared with wild-type by SPSS 11.5 analysis (Student's t-test, P≤0.01). Values are expressed as means (n = 3 leaves for each test), error bars denote SD. ** P≤0.01 for t test. WT wild-type, 35S::PsnAP1-1 and 35S::PsnAP1-2 the different transgenic lines. Chla chlorophyll a, Chlb chlorophyll b, Car carotenoid. B Starch-iodine staining analysis. After 3 h culture under normal light condition, the leaves were removed for starch-iodine staining analysis. WT wild-type, PsnAP1-1 35S::PsnAP1-1 tobacco, PsnAP1-2 35S::PsnAP1-2 tobacco.

Mentions: The leaves of transgenic plants were darker green than those of wild type. Because chlorophyll gives leaves their green color, the difference of chlorophyll content in wild-type and transgenic plants was compared. Results showed significant differences in the total chlorophyll content (chlorophyll a and b content) and the carotenoid content between young transgenic and young wild-type plants (Fig. 7A). The transgenic leaves also exhibited stronger starch-iodine staining than wild-type leaves (Fig. 7B), indicating higher photosynthesis rate in these transgenics than in the wild type.


Overexpression of two PsnAP1 genes from Populus simonii × P. nigra causes early flowering in transgenic tobacco and Arabidopsis.

Zheng T, Li S, Zang L, Dai L, Yang C, Qu GZ - PLoS ONE (2014)

Chlorophyll content and photosynthesis.(A) Chlorophyll content. The leaves were collect from 30-day-old tobacco plant. Each transgenic line showed significant differences compared with wild-type by SPSS 11.5 analysis (Student's t-test, P≤0.01). Values are expressed as means (n = 3 leaves for each test), error bars denote SD. ** P≤0.01 for t test. WT wild-type, 35S::PsnAP1-1 and 35S::PsnAP1-2 the different transgenic lines. Chla chlorophyll a, Chlb chlorophyll b, Car carotenoid. B Starch-iodine staining analysis. After 3 h culture under normal light condition, the leaves were removed for starch-iodine staining analysis. WT wild-type, PsnAP1-1 35S::PsnAP1-1 tobacco, PsnAP1-2 35S::PsnAP1-2 tobacco.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216142&req=5

pone-0111725-g007: Chlorophyll content and photosynthesis.(A) Chlorophyll content. The leaves were collect from 30-day-old tobacco plant. Each transgenic line showed significant differences compared with wild-type by SPSS 11.5 analysis (Student's t-test, P≤0.01). Values are expressed as means (n = 3 leaves for each test), error bars denote SD. ** P≤0.01 for t test. WT wild-type, 35S::PsnAP1-1 and 35S::PsnAP1-2 the different transgenic lines. Chla chlorophyll a, Chlb chlorophyll b, Car carotenoid. B Starch-iodine staining analysis. After 3 h culture under normal light condition, the leaves were removed for starch-iodine staining analysis. WT wild-type, PsnAP1-1 35S::PsnAP1-1 tobacco, PsnAP1-2 35S::PsnAP1-2 tobacco.
Mentions: The leaves of transgenic plants were darker green than those of wild type. Because chlorophyll gives leaves their green color, the difference of chlorophyll content in wild-type and transgenic plants was compared. Results showed significant differences in the total chlorophyll content (chlorophyll a and b content) and the carotenoid content between young transgenic and young wild-type plants (Fig. 7A). The transgenic leaves also exhibited stronger starch-iodine staining than wild-type leaves (Fig. 7B), indicating higher photosynthesis rate in these transgenics than in the wild type.

Bottom Line: Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering.These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11.Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China.

ABSTRACT
In Arabidopsis, AP1 is a floral meristem identity gene and plays an important role in floral organ development. In this study, PsnAP1-1 and PsnAP1-2 were isolated from the male reproductive buds of poplar (Populus simonii × P. nigra), which are the orthologs of AP1 in Arabidopsis, by sequence analysis. Northern blot and qRT-PCR analysis showed that PsnAP1-1 and PsnAP1-2 exhibited high expression level in early inflorescence development of poplar. Subcellular localization showed the PsnAP1-1 and PsnAP1-2 proteins are localized in the nucleus. Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering. These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11. Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent. This study indicates that PsnAP1-1 and PsnAP1-2 play a role in floral transition of poplar.

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