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Overexpression of two PsnAP1 genes from Populus simonii × P. nigra causes early flowering in transgenic tobacco and Arabidopsis.

Zheng T, Li S, Zang L, Dai L, Yang C, Qu GZ - PLoS ONE (2014)

Bottom Line: Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering.These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11.Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China.

ABSTRACT
In Arabidopsis, AP1 is a floral meristem identity gene and plays an important role in floral organ development. In this study, PsnAP1-1 and PsnAP1-2 were isolated from the male reproductive buds of poplar (Populus simonii × P. nigra), which are the orthologs of AP1 in Arabidopsis, by sequence analysis. Northern blot and qRT-PCR analysis showed that PsnAP1-1 and PsnAP1-2 exhibited high expression level in early inflorescence development of poplar. Subcellular localization showed the PsnAP1-1 and PsnAP1-2 proteins are localized in the nucleus. Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering. These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11. Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent. This study indicates that PsnAP1-1 and PsnAP1-2 play a role in floral transition of poplar.

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Subcellular localization analysis of the PsnAP1-1 and PsnAP1-2.PsnAP1-1-GFP, PsnAP1-2-GFP fusion, and GFP alone were each expressed transiently under the control of a CaMV 35S promoter in onion epidermal cells and observed under a confocal microscope. The photographs were taken in a dark field (A, D, G), so that green fluorescence could be used to determine localization, in a bright field to examine cell morphology (B, E, H), and in combination (C, F, I). (A, B, C) The cell is transiently expressing the GFP control. (D, E, F) The cell is expressing the PsnAP1-1-GFP fusion protein. (G, H, I) The cell is expressing the PsnAP1-2-GFP fusion protein.
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pone-0111725-g002: Subcellular localization analysis of the PsnAP1-1 and PsnAP1-2.PsnAP1-1-GFP, PsnAP1-2-GFP fusion, and GFP alone were each expressed transiently under the control of a CaMV 35S promoter in onion epidermal cells and observed under a confocal microscope. The photographs were taken in a dark field (A, D, G), so that green fluorescence could be used to determine localization, in a bright field to examine cell morphology (B, E, H), and in combination (C, F, I). (A, B, C) The cell is transiently expressing the GFP control. (D, E, F) The cell is expressing the PsnAP1-1-GFP fusion protein. (G, H, I) The cell is expressing the PsnAP1-2-GFP fusion protein.

Mentions: The subcellular localization of the PsnAP1 proteins was examined by introduction of the PsnAP1-GFP fusion protein into onion epidermal cells by particle bombardment. While the control GFP fluorescence signals were both detected in cytoplasm and nucleus (Fig. 2A, B, and C), PsnAP1-1-GFP signals were only observed in the nucleus (Fig. 2D, E, and F). Similarly, PsnAP1-2-GFP fluorescence signals were also only detected in the nucleus (Fig. 2G, H, and I).


Overexpression of two PsnAP1 genes from Populus simonii × P. nigra causes early flowering in transgenic tobacco and Arabidopsis.

Zheng T, Li S, Zang L, Dai L, Yang C, Qu GZ - PLoS ONE (2014)

Subcellular localization analysis of the PsnAP1-1 and PsnAP1-2.PsnAP1-1-GFP, PsnAP1-2-GFP fusion, and GFP alone were each expressed transiently under the control of a CaMV 35S promoter in onion epidermal cells and observed under a confocal microscope. The photographs were taken in a dark field (A, D, G), so that green fluorescence could be used to determine localization, in a bright field to examine cell morphology (B, E, H), and in combination (C, F, I). (A, B, C) The cell is transiently expressing the GFP control. (D, E, F) The cell is expressing the PsnAP1-1-GFP fusion protein. (G, H, I) The cell is expressing the PsnAP1-2-GFP fusion protein.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216142&req=5

pone-0111725-g002: Subcellular localization analysis of the PsnAP1-1 and PsnAP1-2.PsnAP1-1-GFP, PsnAP1-2-GFP fusion, and GFP alone were each expressed transiently under the control of a CaMV 35S promoter in onion epidermal cells and observed under a confocal microscope. The photographs were taken in a dark field (A, D, G), so that green fluorescence could be used to determine localization, in a bright field to examine cell morphology (B, E, H), and in combination (C, F, I). (A, B, C) The cell is transiently expressing the GFP control. (D, E, F) The cell is expressing the PsnAP1-1-GFP fusion protein. (G, H, I) The cell is expressing the PsnAP1-2-GFP fusion protein.
Mentions: The subcellular localization of the PsnAP1 proteins was examined by introduction of the PsnAP1-GFP fusion protein into onion epidermal cells by particle bombardment. While the control GFP fluorescence signals were both detected in cytoplasm and nucleus (Fig. 2A, B, and C), PsnAP1-1-GFP signals were only observed in the nucleus (Fig. 2D, E, and F). Similarly, PsnAP1-2-GFP fluorescence signals were also only detected in the nucleus (Fig. 2G, H, and I).

Bottom Line: Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering.These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11.Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China.

ABSTRACT
In Arabidopsis, AP1 is a floral meristem identity gene and plays an important role in floral organ development. In this study, PsnAP1-1 and PsnAP1-2 were isolated from the male reproductive buds of poplar (Populus simonii × P. nigra), which are the orthologs of AP1 in Arabidopsis, by sequence analysis. Northern blot and qRT-PCR analysis showed that PsnAP1-1 and PsnAP1-2 exhibited high expression level in early inflorescence development of poplar. Subcellular localization showed the PsnAP1-1 and PsnAP1-2 proteins are localized in the nucleus. Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering. These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11. Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent. This study indicates that PsnAP1-1 and PsnAP1-2 play a role in floral transition of poplar.

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