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MBP-positive and CD11c-positive cells are associated with different phenotypes of Korean patients with non-asthmatic chronic rhinosinusitis.

Kim DK, Park MH, Chang DY, Eun KM, Shin HW, Mo JH, Shin EC, Jin HR, Shin S, Roh EY, Han DH, Kim DW - PLoS ONE (2014)

Bottom Line: Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP(+), CD163(+), CD11c(+), 2D7(+) and HNE(+) cells, whereas fewer CD68(+) cells were found in NP.Moreover, in UP from CRSwNP, the number of MBP(+) and CD11c(+) cells positively correlated with CT scores.The infiltration of MBP(+) and CD11c(+) innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, Korea.

ABSTRACT

Background: Asthmatic nasal polyps primarily exhibit eosinophilic infiltration. However, the identities of the immune cells that infiltrate non-asthmatic nasal polyps remain unclear. Thus, we thought to investigate the distribution of innate immune cells and its clinical relevance in non-asthmatic chronic rhinosinusitis (CRS) in Korea.

Methods: Tissues from uncinate process (UP) were obtained from controls (n = 18) and CRS without nasal polyps (CRSsNP, n = 45). Nasal polyps (NP) and UP were obtained from CRS with nasal polyps (CRSwNP, n = 56). The innate immune cells was evaluated by immunohistochemistry such as, eosinophil major basic protein (MBP), tryptase, CD68, CD163, CD11c, 2D7, human neutrophil elastase (HNE) and its distribution was analyzed according to clinical parameters.

Results: In comparisons between UP from each group, CRSwNP had a higher number of MPB(+), CD68(+), and CD11c(+) cells relative to CRSsNP. Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP(+), CD163(+), CD11c(+), 2D7(+) and HNE(+) cells, whereas fewer CD68(+) cells were found in NP. In addition, MBP(+) and CD11c(+) cells were increased from UP of CRSsNP, to UP of CRSwNP, and to NP of CRSwNP. Moreover, in UP from CRSwNP, the number of MBP(+) and CD11c(+) cells positively correlated with CT scores. In the analysis of CRSwNP phenotype, allergic eosinophilic polyps had a higher number of MBP(+), tryptase(+), CD11c(+), 2D7(+) cells than others, whereas allergic non-eosinophilic polyps showed mainly infiltration of HNE(+) and 2D7(+) cells.

Conclusions: The infiltration of MBP(+) and CD11c(+) innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.

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Comparison of MBP+ cell, tryptase+ cell, CD68+ cell, CD163+ cell, CD11c+ cell, 2D7+ cell, and HNE+ cell were performed in nasal polyp tissues among four groups: non-eosinophilic non-allergic (NE-NA-NP), non-eosinophilic allergic (NE-A-NP), eosinophilic allergic (E-NA-NP), eosinophilic allergic NP (E-A-NP) (*P<.05, **P<.01, ***P<.001).
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pone-0111352-g004: Comparison of MBP+ cell, tryptase+ cell, CD68+ cell, CD163+ cell, CD11c+ cell, 2D7+ cell, and HNE+ cell were performed in nasal polyp tissues among four groups: non-eosinophilic non-allergic (NE-NA-NP), non-eosinophilic allergic (NE-A-NP), eosinophilic allergic (E-NA-NP), eosinophilic allergic NP (E-A-NP) (*P<.05, **P<.01, ***P<.001).

Mentions: We evaluated the distribution patterns of innate immune cells in different types of CRSwNP. As illustrated in Figure 3, there was a significant higher count of MBP+, tryptase+, CD163+, and CD11c+ cells in eosinophilic NP compared with non-eosinophilic NP. Compared with non-allergic NP, the numbers of MBP+, tryptase+, CD163+, and CD11c+ cells were also significantly increased in allergic NP. Meanwhile, in non-eosinophilic NP, overall innate immune cells were decreased compared with eosinophilic or allergic NP. Furthermore, in comparison between UP from CRSwNP and NP from non-eosinophilic NP, the number of HNE+ cells and CD11c+ cells were significantly higher in NP tissues and UP tissues. However, we found that less number of CD68+ cells was present in NP tissues. Based on the above results, we classified NP samples into four groups: non-eosinophilic non-allergic, non-eosinophilic allergic, eosinophilic non-allergic or eosinophilic allergic (Fig. 4). The frequency of MBP+ cell infiltration was higher in eosinophilic allergic NP than in non-eosinophilic non-allergic NP (Fig. 4A). Moreover, eosinophilic allergic NP had more tryptase+ cells, CD11c+ cells, and 2D7+ cells than observed in other types of CRSwNP (Fig. 4B, 4E, and 4F). In addition, compared with non-eosinophilic non-allergic NP and eosinophilic non-allergic NP, significantly increased numbers of 2D7+ cells and HNE+ cells were found in non-eosinophilic allergic NP (Fig. 4F and 4G). These data imply that allergic status influence cellular distribution in eosinophilic or non-eosinophilic NP.


MBP-positive and CD11c-positive cells are associated with different phenotypes of Korean patients with non-asthmatic chronic rhinosinusitis.

Kim DK, Park MH, Chang DY, Eun KM, Shin HW, Mo JH, Shin EC, Jin HR, Shin S, Roh EY, Han DH, Kim DW - PLoS ONE (2014)

Comparison of MBP+ cell, tryptase+ cell, CD68+ cell, CD163+ cell, CD11c+ cell, 2D7+ cell, and HNE+ cell were performed in nasal polyp tissues among four groups: non-eosinophilic non-allergic (NE-NA-NP), non-eosinophilic allergic (NE-A-NP), eosinophilic allergic (E-NA-NP), eosinophilic allergic NP (E-A-NP) (*P<.05, **P<.01, ***P<.001).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216068&req=5

pone-0111352-g004: Comparison of MBP+ cell, tryptase+ cell, CD68+ cell, CD163+ cell, CD11c+ cell, 2D7+ cell, and HNE+ cell were performed in nasal polyp tissues among four groups: non-eosinophilic non-allergic (NE-NA-NP), non-eosinophilic allergic (NE-A-NP), eosinophilic allergic (E-NA-NP), eosinophilic allergic NP (E-A-NP) (*P<.05, **P<.01, ***P<.001).
Mentions: We evaluated the distribution patterns of innate immune cells in different types of CRSwNP. As illustrated in Figure 3, there was a significant higher count of MBP+, tryptase+, CD163+, and CD11c+ cells in eosinophilic NP compared with non-eosinophilic NP. Compared with non-allergic NP, the numbers of MBP+, tryptase+, CD163+, and CD11c+ cells were also significantly increased in allergic NP. Meanwhile, in non-eosinophilic NP, overall innate immune cells were decreased compared with eosinophilic or allergic NP. Furthermore, in comparison between UP from CRSwNP and NP from non-eosinophilic NP, the number of HNE+ cells and CD11c+ cells were significantly higher in NP tissues and UP tissues. However, we found that less number of CD68+ cells was present in NP tissues. Based on the above results, we classified NP samples into four groups: non-eosinophilic non-allergic, non-eosinophilic allergic, eosinophilic non-allergic or eosinophilic allergic (Fig. 4). The frequency of MBP+ cell infiltration was higher in eosinophilic allergic NP than in non-eosinophilic non-allergic NP (Fig. 4A). Moreover, eosinophilic allergic NP had more tryptase+ cells, CD11c+ cells, and 2D7+ cells than observed in other types of CRSwNP (Fig. 4B, 4E, and 4F). In addition, compared with non-eosinophilic non-allergic NP and eosinophilic non-allergic NP, significantly increased numbers of 2D7+ cells and HNE+ cells were found in non-eosinophilic allergic NP (Fig. 4F and 4G). These data imply that allergic status influence cellular distribution in eosinophilic or non-eosinophilic NP.

Bottom Line: Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP(+), CD163(+), CD11c(+), 2D7(+) and HNE(+) cells, whereas fewer CD68(+) cells were found in NP.Moreover, in UP from CRSwNP, the number of MBP(+) and CD11c(+) cells positively correlated with CT scores.The infiltration of MBP(+) and CD11c(+) innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, Korea.

ABSTRACT

Background: Asthmatic nasal polyps primarily exhibit eosinophilic infiltration. However, the identities of the immune cells that infiltrate non-asthmatic nasal polyps remain unclear. Thus, we thought to investigate the distribution of innate immune cells and its clinical relevance in non-asthmatic chronic rhinosinusitis (CRS) in Korea.

Methods: Tissues from uncinate process (UP) were obtained from controls (n = 18) and CRS without nasal polyps (CRSsNP, n = 45). Nasal polyps (NP) and UP were obtained from CRS with nasal polyps (CRSwNP, n = 56). The innate immune cells was evaluated by immunohistochemistry such as, eosinophil major basic protein (MBP), tryptase, CD68, CD163, CD11c, 2D7, human neutrophil elastase (HNE) and its distribution was analyzed according to clinical parameters.

Results: In comparisons between UP from each group, CRSwNP had a higher number of MPB(+), CD68(+), and CD11c(+) cells relative to CRSsNP. Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP(+), CD163(+), CD11c(+), 2D7(+) and HNE(+) cells, whereas fewer CD68(+) cells were found in NP. In addition, MBP(+) and CD11c(+) cells were increased from UP of CRSsNP, to UP of CRSwNP, and to NP of CRSwNP. Moreover, in UP from CRSwNP, the number of MBP(+) and CD11c(+) cells positively correlated with CT scores. In the analysis of CRSwNP phenotype, allergic eosinophilic polyps had a higher number of MBP(+), tryptase(+), CD11c(+), 2D7(+) cells than others, whereas allergic non-eosinophilic polyps showed mainly infiltration of HNE(+) and 2D7(+) cells.

Conclusions: The infiltration of MBP(+) and CD11c(+) innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.

Show MeSH
Related in: MedlinePlus