Granulin-epithelin precursor interacts with heparan sulfate on liver cancer cells.
Bottom Line: Suppression of the HS polymerase exostosin-1 reduced the rGEP binding and rGEP-mediated signaling transduction.Suppression of a specific HS proteoglycan, glypican-3, also showed a partial reduction of rGEP binding and an inhibition on rGEP-mediated activation of AKT.Furthermore, glypican-3 was shown to correlate with the expressions of GEP in clinical samples (Spearman's ρ = 0.363, P = 0.001).
Affiliation: Department of Surgery, Centre for Cancer Research and.Show MeSH
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Mentions: GPC3 is one of the HSPGs located on the cell surface and was found upregulated in HCC tumors (27). The mRNA expression levels of GPC3 were investigated in 77 pairs of HCC and adjacent non-tumor liver tissues, as well as 10 normal liver tissues. Elevated GPC3 transcript levels were observed in tumor compared with non-tumor tissues and normal liver tissues (P < 0.001) (Figure 5A). The GEP and GPC3 levels were significantly correlated (n = 77, Spearman’s ρ correlation coefficient = 0.363, P = 0.001) (Figure 5B), corroborating their biological functions as binding partners.
Affiliation: Department of Surgery, Centre for Cancer Research and.