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Downregulated Ku70 and ATM associated to poor prognosis in colorectal cancer among Chinese patients.

Lu Y, Gao J, Lu Y - Onco Targets Ther (2014)

Bottom Line: Downexpression patterns for both Ku70 and ATM were found in the CRC samples and were significantly associated with advanced tumor node metastasis stage and decreased 5-year overall survival rate.Downregulated Ku70 and ATM were associated with poor disease-free survival.Loss of Ku70 and ATM expression might act as a biomarker to predict poor prognosis in patients with CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, School of Public Health, Guilin Medical University, Guangxi, People's Republic of China ; Department of Clinical Research Center, Affiliated 2nd Hospital of Nanjing Medical University, Nanjing, People's Republic of China.

ABSTRACT

Background: Double-strand DNA breaks (DSBs) are a key factor in carcinogenesis. The necessary repair of DSBs is pivotal in maintaining normal cell division. To address the relationship between altered expression of DSB repair of proteins Ku70 and ataxia-telangiectasia mutated (ATM) in colorectal cancer (CRC), we examined the expression levels and patterns of Ku70 and ATM in CRC samples.

Methods: Expression and coexpression of Ku70 and ATM were investigated by using real-time quantitative polymerase chain reaction assays and confirmed further with fluorescent immunohistochemistry in CRC and pericancerous samples from 112 Chinese patients.

Results: Downexpression patterns for both Ku70 and ATM were found in the CRC samples and were significantly associated with advanced tumor node metastasis stage and decreased 5-year overall survival rate.

Conclusion: Downregulated Ku70 and ATM were associated with poor disease-free survival. Loss of Ku70 and ATM expression might act as a biomarker to predict poor prognosis in patients with CRC.

No MeSH data available.


Related in: MedlinePlus

Ku70 and ATM expression in the colorectal cancer and peri-cancer tissues.Notes: (A) Western blot analysis of Ku70 and ATM expression was studied in different group of cancer (C) and peri-cancer normal tissue (N). **P<0.01. (B) Quantitative real-time PCR analysis of Ku70 and ATM in 112 colorectal cancer and normal tissues. Quantified data were normalized to the housekeeping control of β-actin. (C) Correlation between Ku70 and ATM expression in the real-time QPCR assay (**P<0.01).Abbreviations: ATM, ataxia-telangiectasia mutated; PCR, polymerase chain reaction; QPCR, quantitative polymerase chain reaction; mRNA, messenger RNA.
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f1-ott-7-1955: Ku70 and ATM expression in the colorectal cancer and peri-cancer tissues.Notes: (A) Western blot analysis of Ku70 and ATM expression was studied in different group of cancer (C) and peri-cancer normal tissue (N). **P<0.01. (B) Quantitative real-time PCR analysis of Ku70 and ATM in 112 colorectal cancer and normal tissues. Quantified data were normalized to the housekeeping control of β-actin. (C) Correlation between Ku70 and ATM expression in the real-time QPCR assay (**P<0.01).Abbreviations: ATM, ataxia-telangiectasia mutated; PCR, polymerase chain reaction; QPCR, quantitative polymerase chain reaction; mRNA, messenger RNA.

Mentions: Real-time QPCR was used to analyze Ku70 and ATM expression in CRC and pericancerous normal tissue. The relative gene expression quantifications were calculated according to the comparative Ct method using β-actin as an endogenous control. Both Ku70 and ATM mRNA expressions were downregulated in CRC compared to pericancerous normal tissues. Correlations of Ku70 to ATM expression levels (R2=0.9364; P<0.001) were calculated with relative expression level of Ku70 and ATM in cancer group separately, and the results are shown in Figure 1. The coexpression pattern of downregulation of Ku70 and ATM was confirmed further in the CRC samples with fluorescent IHC staining. Confocal microscopy showed a pattern of coexpressed Ku70 and ATM. Downregulation of Ku70 and ATM was observed in cancer tissues compared to pericancerous tissues (data not shown).


Downregulated Ku70 and ATM associated to poor prognosis in colorectal cancer among Chinese patients.

Lu Y, Gao J, Lu Y - Onco Targets Ther (2014)

Ku70 and ATM expression in the colorectal cancer and peri-cancer tissues.Notes: (A) Western blot analysis of Ku70 and ATM expression was studied in different group of cancer (C) and peri-cancer normal tissue (N). **P<0.01. (B) Quantitative real-time PCR analysis of Ku70 and ATM in 112 colorectal cancer and normal tissues. Quantified data were normalized to the housekeeping control of β-actin. (C) Correlation between Ku70 and ATM expression in the real-time QPCR assay (**P<0.01).Abbreviations: ATM, ataxia-telangiectasia mutated; PCR, polymerase chain reaction; QPCR, quantitative polymerase chain reaction; mRNA, messenger RNA.
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Related In: Results  -  Collection

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f1-ott-7-1955: Ku70 and ATM expression in the colorectal cancer and peri-cancer tissues.Notes: (A) Western blot analysis of Ku70 and ATM expression was studied in different group of cancer (C) and peri-cancer normal tissue (N). **P<0.01. (B) Quantitative real-time PCR analysis of Ku70 and ATM in 112 colorectal cancer and normal tissues. Quantified data were normalized to the housekeeping control of β-actin. (C) Correlation between Ku70 and ATM expression in the real-time QPCR assay (**P<0.01).Abbreviations: ATM, ataxia-telangiectasia mutated; PCR, polymerase chain reaction; QPCR, quantitative polymerase chain reaction; mRNA, messenger RNA.
Mentions: Real-time QPCR was used to analyze Ku70 and ATM expression in CRC and pericancerous normal tissue. The relative gene expression quantifications were calculated according to the comparative Ct method using β-actin as an endogenous control. Both Ku70 and ATM mRNA expressions were downregulated in CRC compared to pericancerous normal tissues. Correlations of Ku70 to ATM expression levels (R2=0.9364; P<0.001) were calculated with relative expression level of Ku70 and ATM in cancer group separately, and the results are shown in Figure 1. The coexpression pattern of downregulation of Ku70 and ATM was confirmed further in the CRC samples with fluorescent IHC staining. Confocal microscopy showed a pattern of coexpressed Ku70 and ATM. Downregulation of Ku70 and ATM was observed in cancer tissues compared to pericancerous tissues (data not shown).

Bottom Line: Downexpression patterns for both Ku70 and ATM were found in the CRC samples and were significantly associated with advanced tumor node metastasis stage and decreased 5-year overall survival rate.Downregulated Ku70 and ATM were associated with poor disease-free survival.Loss of Ku70 and ATM expression might act as a biomarker to predict poor prognosis in patients with CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, School of Public Health, Guilin Medical University, Guangxi, People's Republic of China ; Department of Clinical Research Center, Affiliated 2nd Hospital of Nanjing Medical University, Nanjing, People's Republic of China.

ABSTRACT

Background: Double-strand DNA breaks (DSBs) are a key factor in carcinogenesis. The necessary repair of DSBs is pivotal in maintaining normal cell division. To address the relationship between altered expression of DSB repair of proteins Ku70 and ataxia-telangiectasia mutated (ATM) in colorectal cancer (CRC), we examined the expression levels and patterns of Ku70 and ATM in CRC samples.

Methods: Expression and coexpression of Ku70 and ATM were investigated by using real-time quantitative polymerase chain reaction assays and confirmed further with fluorescent immunohistochemistry in CRC and pericancerous samples from 112 Chinese patients.

Results: Downexpression patterns for both Ku70 and ATM were found in the CRC samples and were significantly associated with advanced tumor node metastasis stage and decreased 5-year overall survival rate.

Conclusion: Downregulated Ku70 and ATM were associated with poor disease-free survival. Loss of Ku70 and ATM expression might act as a biomarker to predict poor prognosis in patients with CRC.

No MeSH data available.


Related in: MedlinePlus