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Retinal thickness measurement obtained with spectral domain optical coherence tomography assisted optical biopsy accurately correlates with ex vivo histology.

Ferguson LR, Grover S, Dominguez JM, Balaiya S, Chalam KV - PLoS ONE (2014)

Bottom Line: The retinal thickness for all sublayers measured by SD-OCT vs. histology were also similar, the 'correlation constant' values ranged from 0.70 to 1.17.All SD-OCT and histological measurements demonstrated highly significant (p<0.01) strong positive correlations.This study establishes conversion factors for the translation of ex vivo data into in vivo information; thus enhancing the applicability of SD-OCT in translational research.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Florida College of Medicine, Jacksonville, Florida, United States of America.

ABSTRACT

Background: This study determines 'correlation constants' between the gold standard histological measurement of retinal thickness and the newer spectral-domain optical coherence tomography (SD-OCT) technology in adult C57BL/6 mice.

Methods: Forty-eight eyes from adult mice underwent SD-OCT imaging and then were histologically prepared for frozen sectioning with H&E staining. Retinal thickness was measured via 10x light microscopy. SD-OCT images and histological sections were standardized to three anatomical sites relative to the optic nerve head (ONH) location. The ratios between SD-OCT to histological thickness for total retinal thickness (TRT) and six sublayers were defined as 'correlation constants'.

Results: Mean (± SE) TRT for SD-OCT and histological sections was 210.95 µm (± 1.09) and 219.58 µm (± 2.67), respectively. The mean 'correlation constant' for TRT between the SD-OCT and histological sections was 0.96. The retinal thickness for all sublayers measured by SD-OCT vs. histology were also similar, the 'correlation constant' values ranged from 0.70 to 1.17. All SD-OCT and histological measurements demonstrated highly significant (p<0.01) strong positive correlations.

Conclusion: This study establishes conversion factors for the translation of ex vivo data into in vivo information; thus enhancing the applicability of SD-OCT in translational research.

Show MeSH
Association between mean SD-OCT and histological thickness with correlation constants >1.Scatter plot points refer to all observations for outer nuclear layer and retinal nerve fiber layer.
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pone-0111203-g003: Association between mean SD-OCT and histological thickness with correlation constants >1.Scatter plot points refer to all observations for outer nuclear layer and retinal nerve fiber layer.

Mentions: Sublayer analyses showed that the ‘correlation constant’ of SD-OCT to histology thickness was greater than one for the ONL (1.14) and RNFL (1.17) and was less than one for the OS/IS/ELM (0.91), OPL (0.87), INL (0.7), and IPL/GC (0.8) layers (Tables 2&3). In general, thickness measurement comparisons between SD-OCT and histology demonstrated significant correlations. The ONL and RNFL, with ‘correlation constants’ greater than one, showed a highly significant strong correlation (r2 = 0.98, p<0.01) [Figure 3]. Similarly, for the layers with ‘correlation constants’ less than one, the relationship exhibited between SD-OCT and histological measurements was also significantly correlated (r2 = 0.99, p<0.01) [Figure 4].


Retinal thickness measurement obtained with spectral domain optical coherence tomography assisted optical biopsy accurately correlates with ex vivo histology.

Ferguson LR, Grover S, Dominguez JM, Balaiya S, Chalam KV - PLoS ONE (2014)

Association between mean SD-OCT and histological thickness with correlation constants >1.Scatter plot points refer to all observations for outer nuclear layer and retinal nerve fiber layer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4216007&req=5

pone-0111203-g003: Association between mean SD-OCT and histological thickness with correlation constants >1.Scatter plot points refer to all observations for outer nuclear layer and retinal nerve fiber layer.
Mentions: Sublayer analyses showed that the ‘correlation constant’ of SD-OCT to histology thickness was greater than one for the ONL (1.14) and RNFL (1.17) and was less than one for the OS/IS/ELM (0.91), OPL (0.87), INL (0.7), and IPL/GC (0.8) layers (Tables 2&3). In general, thickness measurement comparisons between SD-OCT and histology demonstrated significant correlations. The ONL and RNFL, with ‘correlation constants’ greater than one, showed a highly significant strong correlation (r2 = 0.98, p<0.01) [Figure 3]. Similarly, for the layers with ‘correlation constants’ less than one, the relationship exhibited between SD-OCT and histological measurements was also significantly correlated (r2 = 0.99, p<0.01) [Figure 4].

Bottom Line: The retinal thickness for all sublayers measured by SD-OCT vs. histology were also similar, the 'correlation constant' values ranged from 0.70 to 1.17.All SD-OCT and histological measurements demonstrated highly significant (p<0.01) strong positive correlations.This study establishes conversion factors for the translation of ex vivo data into in vivo information; thus enhancing the applicability of SD-OCT in translational research.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Florida College of Medicine, Jacksonville, Florida, United States of America.

ABSTRACT

Background: This study determines 'correlation constants' between the gold standard histological measurement of retinal thickness and the newer spectral-domain optical coherence tomography (SD-OCT) technology in adult C57BL/6 mice.

Methods: Forty-eight eyes from adult mice underwent SD-OCT imaging and then were histologically prepared for frozen sectioning with H&E staining. Retinal thickness was measured via 10x light microscopy. SD-OCT images and histological sections were standardized to three anatomical sites relative to the optic nerve head (ONH) location. The ratios between SD-OCT to histological thickness for total retinal thickness (TRT) and six sublayers were defined as 'correlation constants'.

Results: Mean (± SE) TRT for SD-OCT and histological sections was 210.95 µm (± 1.09) and 219.58 µm (± 2.67), respectively. The mean 'correlation constant' for TRT between the SD-OCT and histological sections was 0.96. The retinal thickness for all sublayers measured by SD-OCT vs. histology were also similar, the 'correlation constant' values ranged from 0.70 to 1.17. All SD-OCT and histological measurements demonstrated highly significant (p<0.01) strong positive correlations.

Conclusion: This study establishes conversion factors for the translation of ex vivo data into in vivo information; thus enhancing the applicability of SD-OCT in translational research.

Show MeSH